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Literature summary for 2.4.1.19 extracted from

  • Nakamura, N.; Horikoshi, K.
    Purification and properties of neutral-cyclodextrin glycosyl-transferase of an alkalophilic Bacillus sp. (1976), Agric. Biol. Chem., 40, 1785-1791.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
beta-cyclodextrin ATCC 21783, acid CGTase Niallia circulans
0.83
-
beta-cyclodextrin ATCC 21783 Niallia circulans
5.9
-
alpha-cyclodextrin ATCC 21783, acid CGTase Niallia circulans
10
-
alpha-cyclodextrin ATCC 21783 Niallia circulans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Geobacillus stearothermophilus
Ca2+
-
Bacillus sp. (in: Bacteria)
Ca2+
-
Paenibacillus macerans
Ca2+ addition increases thermal stability Niallia circulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000 88000 ATCC 21783, gel filtration, SDS-PAGE Niallia circulans

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Geobacillus stearothermophilus
-
-
-
Niallia circulans
-
B. sp. var. alkalophilus No. 38-2, ATCC 21783
-
Paenibacillus macerans
-
-
-
Priestia megaterium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ATCC 21783 Niallia circulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
281
-
ATCC 21783 Niallia circulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-cyclodextrin + sucrose ATCC 21783 Niallia circulans ?
-
r
amylopectin + glycosyl acceptor
-
Niallia circulans cycloheptaamylose + cyclohexaamylose + exo-branched cyclohexaamylose
-
r
amylopectin beta-limit dextrin + glycosyl acceptor
-
Niallia circulans ?
-
r
amylose + glycosyl acceptor
-
Geobacillus stearothermophilus ?
-
r
amylose + glycosyl acceptor
-
Niallia circulans ?
-
r
beta-cyclodextrin + maltose
-
Paenibacillus macerans alpha-cyclodextrin + maltooligosaccharide
-
r
beta-cyclodextrin + maltose ATCC 21783 Niallia circulans alpha-cyclodextrin + maltooligosaccharide
-
r
beta-cyclodextrin + maltotriose ATCC 21783 Niallia circulans alpha-cyclodextrin + maltooligosaccharide
-
r
beta-cyclodextrin + sucrose ATCC 21783 Niallia circulans ?
-
r
glycogen + acceptor
-
Geobacillus stearothermophilus beta-cyclodextrin
-
?
glycogen + acceptor
-
Paenibacillus macerans beta-cyclodextrin
-
r
glycogen + acceptor ATCC 21783 Niallia circulans beta-cyclodextrin
-
r
maltose + glycosyl acceptor
-
Geobacillus stearothermophilus beta-cyclodextrin
-
?
maltose + glycosyl acceptor ATCC 21783 Niallia circulans beta-cyclodextrin
-
?
maltotriose + glycosyl acceptor
-
Geobacillus stearothermophilus beta-cyclodextrin
-
?
maltotriose + glycosyl acceptor ATCC 21783 Niallia circulans beta-cyclodextrin
-
?
soluble starch + glycosyl acceptor
-
Bacillus sp. (in: Bacteria) Schardinger beta-dextrin
-
?
soluble starch + glycosyl acceptor ATCC 21783 Niallia circulans Schardinger beta-dextrin
-
?
soluble starch + glycosyl acceptor
-
Geobacillus stearothermophilus alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Priestia megaterium alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Bacillus sp. (in: Bacteria) alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Paenibacillus macerans alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Niallia circulans alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin 21783, neutral CGTase, 0.4, 14 and 2.5% r
soluble starch + glycosyl acceptor
-
Bacillus sp. (in: Bacteria) beta-cyclodextrin + gamma-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Niallia circulans beta-cyclodextrin + gamma-cyclodextrin
-
r

Subunits

Subunits Comment Organism
monomer
-
Bacillus sp. (in: Bacteria)
monomer
-
Paenibacillus macerans
monomer 1 * 85000-88000, ATCC 2783, SDS-PAGE Niallia circulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus sp. (in: Bacteria)
50
-
ATCC 21783 Niallia circulans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 70 ATCC 21783 Niallia circulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
-
Geobacillus stearothermophilus
60
-
-
Paenibacillus macerans
60
-
ATCC 21783, stable up to, Ca2+ addition increases thermal stability Niallia circulans
60
-
ATCC 21783, stable up to Niallia circulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5 ATCC 21783, crude enzyme, second pH optimum at 8.0-9.0 Niallia circulans
7
-
-
Priestia megaterium
7
-
-
Paenibacillus macerans
7
-
ATCC 21783, purified enzyme, crude enzyme has 3 pH optima, 4.5-5.0, 7.0 and 8.0-9.0 Niallia circulans
8 9 ATCC 21783, crude enzyme, second pH optimum at 4.5-5.0 Niallia circulans

pH Range

pH Minimum pH Maximum Comment Organism
4 10 ATCC 21783 Niallia circulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 ATCC 21783 Niallia circulans