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Literature summary for 2.4.1.19 extracted from

  • Jeang, C.L.; Wung, C.H.; Chang, B.Y.; Yeh, S.S.; Lour, D.W.
    Characterization of the Bacillus macerans cyclodextrin glucanotransferase overexpressed in Escherichia coli (1999), Proc. Natl. Sci. Counc. Repub. China B, 23, 62-68.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned and overexpressed in Escherichia coli Paenibacillus macerans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.58
-
starch wild-type Paenibacillus macerans
1.05
-
starch mutant E344D Paenibacillus macerans
2.1
-
starch authentic CGTase Paenibacillus macerans

Organism

Organism UniProt Comment Textmining
Paenibacillus macerans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and recombinant enzymes Paenibacillus macerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-1,4-glucan + glycosyl acceptor
-
Paenibacillus macerans cyclodextrins
-
r
soluble starch + glycosyl acceptor
-
Paenibacillus macerans cyclodextrins
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.442
-
starch wild-type Paenibacillus macerans
1.51
-
starch mutant E344D Paenibacillus macerans
1.66
-
starch authentic CGTase Paenibacillus macerans