Cloned (Comment) | Organism |
---|---|
cloned and overexpressed in Escherichia coli | Paenibacillus macerans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.58 | - |
starch | wild-type | Paenibacillus macerans | |
1.05 | - |
starch | mutant E344D | Paenibacillus macerans | |
2.1 | - |
starch | authentic CGTase | Paenibacillus macerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus macerans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and recombinant enzymes | Paenibacillus macerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-1,4-glucan + glycosyl acceptor | - |
Paenibacillus macerans | cyclodextrins | - |
r | |
soluble starch + glycosyl acceptor | - |
Paenibacillus macerans | cyclodextrins | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.442 | - |
starch | wild-type | Paenibacillus macerans | |
1.51 | - |
starch | mutant E344D | Paenibacillus macerans | |
1.66 | - |
starch | authentic CGTase | Paenibacillus macerans |