Cloned (Comment) | Organism |
---|---|
- |
Thermococcus sp. |
cgtA gene cloned and expressed in Escherichia coli JM109 | Thermococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Niallia circulans | - |
- |
- |
Niallia circulans | - |
no. 8 | - |
Niallia circulans 251 | - |
- |
- |
Niallia circulans 8 | - |
no. 8 | - |
Thermoanaerobacterium thermosulfurigenes | - |
EM1 | - |
Thermoanaerobacterium thermosulfurigenes EM1 | - |
EM1 | - |
Thermococcus sp. | - |
B1001 | - |
Thermococcus sp. | Q9UWN2 | B1001, DNA sequence of CGTase gene, EMBL/GenBank/DDBJ database accession No. | - |
Thermococcus sp. B1001 | - |
B1001 | - |
Purification (Comment) | Organism |
---|---|
natural and recombinant enzyme | Thermococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch + glycosyl acceptor | - |
Thermococcus sp. | alpha-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | - |
Thermococcus sp. B1001 | alpha-cyclodextrin | - |
r | |
starch | - |
Thermococcus sp. | beta-cyclodextrin | - |
? | |
starch | - |
Thermococcus sp. B1001 | beta-cyclodextrin | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 107 | - |
Thermococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 110 | above 80°C activity increases by heat treatment, enzyme is irreversibly unfolded above 110°C | Thermococcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
starch | unheated CGTase | Thermococcus sp. | |
1.33 | - |
starch | heated CGTase | Thermococcus sp. |