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Literature summary for 2.4.1.19 extracted from
- Molecular cloning and characterization of a novel gamma-CGTase from alkalophilic Bacillus sp. (2005), Appl. Microbiol. Biotechnol., 70, 193-201.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Bacillus subtilis KN2 | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
G-825-6 | - |
| Bacillus sp. (in: Bacteria) G-825-6 | - |
G-825-6 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) | gamma-cyclodextrin + beta-cyclodextrin | alpha-cyclodextrin production is not observed at any pH examined. The enzyme produces gamma-cyclodextrin principally at any pH and the ratio of gamma-cyclodextrin to beta-cyclodextrin is always more than 1.7 and 4.7 with 1% and 10% substrate | ? |  |
| soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) G-825-6 | gamma-cyclodextrin + beta-cyclodextrin | alpha-cyclodextrin production is not observed at any pH examined. The enzyme produces gamma-cyclodextrin principally at any pH and the ratio of gamma-cyclodextrin to beta-cyclodextrin is always more than 1.7 and 4.7 with 1% and 10% substrate | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | - |
Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 h, enzyme retains more than 90% of the original activity up to 50°C | Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
beta-cyclodextrin production shows increment up to pH 8.0 but decreases at pH values higher than 9 | Bacillus sp. (in: Bacteria) |
| 8 | 10 | gamma-cyclodextrin production, reaction with 10% starch | Bacillus sp. (in: Bacteria) |
| 9 | - |
gamma-cyclodextrin production, reaction with 1% starch | Bacillus sp. (in: Bacteria) |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 12 | 37°C, 30 min, stable | Bacillus sp. (in: Bacteria) |
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Release 2023.1 (January 2023)
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