Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.19 extracted from

  • Kim, W.; Chae, H.; Park, C.; Lee, K.
    Stability and activity of crosslinking enzyme crystals of cyclodextrin glucanotransferase isolated from Bacillus macerans (2003), J. Mol. Catal. B, 26, 287-292.
No PubMed abstract available
Search for more literature for 2.4.1.19

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion technique. Cross-linked enzyme crystals can be useful biocatalysts because they are stable at elevated temperature, in organic solvents, and in the presence of enzyme inactivation surfactant. They also maintain their activity against protein-digesting enzyme Paenibacillus macerans

General Stability

General Stability Organism
cross-linked enzyme crystals are slightly more resistant to 2-butanol and acetonitrile than DMSO. In 15% solution of 2-butanol, over 100% of the activity is retained in CGTase-cross-linking enzyme crystals while 28% of the activity remains in the case of soluble CGTase Paenibacillus macerans
SDS, 1%, only 7% of the activity of soluble enzyme activity remains, cross-linked enzyme crystals exhibit strong activity Paenibacillus macerans

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 5 mM, 68% inhibition of cross-linked enzyme crystals, 40% of soluble enzyme Paenibacillus macerans
MgCl2 5 mM, 15% inhibition of cross-linked enzyme crystals, 22% of soluble enzyme Paenibacillus macerans
ZnCl2 5 mM, 30% inhibition of cross-linked enzyme crystals, 80% of soluble enzyme Paenibacillus macerans

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 5 mM, 20% activation of cross-linked enzyme crystals, 2% activation of soluble enzyme Paenibacillus macerans
FeCl2 5 mM, 110% activation of cross-linked enzyme crystals, inhibition of soluble enzyme Paenibacillus macerans

Organic Solvent Stability

Organic Solvent Comment Organism
2-butanol in 15% solution of 2-butanol, over 100% of the activity is retained in CGTase-cross-linking enzyme crystals while 28% of the activity remains in the case of soluble CGTase Paenibacillus macerans
acetonitrile in 15% solution of 2-butanol, over 100% of the activity is retained in CGTase-cross-linking enzyme crystals while 60% of the activity remains in the case of soluble CGTase Paenibacillus macerans

Organism

Organism UniProt Comment Textmining
Paenibacillus macerans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + glycosyl acceptor
-
 Paenibacillus macerans cyclodextrin
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 soluble enzyme Paenibacillus macerans
60 70 cross-linked enzyme crystals Paenibacillus macerans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 40°C: about 60% of maximal activity, 80°C: about 45% of maximal activity,cross-linked enzyme crystals Paenibacillus macerans
40 75 40°C: about 40% of maximal activity, 75°C: about 15% of maximal activity, soluble enzyme Paenibacillus macerans