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Literature summary for 2.4.1.19 extracted from

  • Charoensakdi, R.; Murakami, S.; Aoki, K.; Rimphanitchayakit, V.; Limpaseni, T.
    Cloning and expression of cyclodextrin glycosyltransferase gene from Paenibacillus sp. T16 isolated from hot spring soil in northern Thailand (2007), J. Biochem. Mol. Biol., 40, 333-340.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of hydrolyzing activity with different substrates, e.g. cyclodextrins, overview Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
x * 77000, native and recombinant enzyme, SDS-PAGE Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp.
-
thermotolerant strain T16 isolated from hot spring soil in northern Thailand
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 99fold and recombinant enzyme 7fold from Escherichia coli both by starch adsorption, and hydrophobic interaction or beta-cyclodextrin affinity chromatography Paenibacillus sp.

Source Tissue

Source Tissue Comment Organism Textmining
additional information optimal growth conditions for wild-type strain T16 and transfected strain pT16, overview Paenibacillus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.7
-
purified native enzyme Paenibacillus sp.
89.7
-
purified recombinant enzyme Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information soluble potato starch, cellobiose, and cyclodextrins as substrates, cyclodextrin product spectrum of native and recombinant enzymes, overview Paenibacillus sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 77000, native and recombinant enzyme, SDS-PAGE Paenibacillus sp.

Synonyms

Synonyms Comment Organism
CGTase
-
Paenibacillus sp.
cyclodextrin glycosyltransferase
-
Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
pT16 shows broader optimum temperatures for both dextrinizing and cyclization activities Paenibacillus sp.
40 60 assay at, dependent on assay method Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the recombinant enzyme is more stable at higher temperature and lower pH Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.9 6.5 the optimum pH for dextrinizing and cyclization activities of purified CGTases from both sources are similar around pH 6.5 and pH 5.9 respectively Paenibacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
the recombinant enzyme is more stable at higher temperature and lower pH Paenibacillus sp.