Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 | Paenibacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of hydrolyzing activity with different substrates, e.g. cyclodextrins, overview | Paenibacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
x * 77000, native and recombinant enzyme, SDS-PAGE | Paenibacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. | - |
thermotolerant strain T16 isolated from hot spring soil in northern Thailand | - |
Purification (Comment) | Organism |
---|---|
native enzyme 99fold and recombinant enzyme 7fold from Escherichia coli both by starch adsorption, and hydrophobic interaction or beta-cyclodextrin affinity chromatography | Paenibacillus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimal growth conditions for wild-type strain T16 and transfected strain pT16, overview | Paenibacillus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.7 | - |
purified native enzyme | Paenibacillus sp. |
89.7 | - |
purified recombinant enzyme | Paenibacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | soluble potato starch, cellobiose, and cyclodextrins as substrates, cyclodextrin product spectrum of native and recombinant enzymes, overview | Paenibacillus sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 77000, native and recombinant enzyme, SDS-PAGE | Paenibacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
CGTase | - |
Paenibacillus sp. |
cyclodextrin glycosyltransferase | - |
Paenibacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
pT16 shows broader optimum temperatures for both dextrinizing and cyclization activities | Paenibacillus sp. |
40 | 60 | assay at, dependent on assay method | Paenibacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the recombinant enzyme is more stable at higher temperature and lower pH | Paenibacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | 6.5 | the optimum pH for dextrinizing and cyclization activities of purified CGTases from both sources are similar around pH 6.5 and pH 5.9 respectively | Paenibacillus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the recombinant enzyme is more stable at higher temperature and lower pH | Paenibacillus sp. |