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Literature summary for 2.4.1.19 extracted from

  • Atanasova, N.; Kitayska, T.; Yankov, D.; Safarikova, M.; Tonkova, A.
    Cyclodextrin glucanotransferase production by cell biocatalysts of alkaliphilic bacilli (2009), Biochem. Eng. J., 46, 278-285.
No PubMed abstract available

Application

Application Comment Organism
synthesis the enzyme as membrane biocatalyst is applied for a direct cyclodextrin production Alkalihalobacillus pseudalcaliphilus

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the enzyme by three different techniques: on two types of polysulfone membranes; entrapped in agar-gel beads containing magnetite, and by nano-particles of silanized magnetite covalently bound on the cell surface. The enzyme immobilized on membrane shows highest enzyme activity and high stability, overview Alkalihalobacillus pseudalcaliphilus

General Stability

General Stability Organism
cell membrane-immobilized purified enzyme retains, after 240 h repeated batch cultivation, 1.3-2.3fold increase of the CGTase yield compared to free cells at the end of the process, overview Alkalihalobacillus pseudalcaliphilus

Organism

Organism UniProt Comment Textmining
Alkalihalobacillus pseudalcaliphilus
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strains 20RF and 8SB, isolated from Bulgarian habitats
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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activities of free and immobilized enzymes, overview Alkalihalobacillus pseudalcaliphilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the immobilized enzyme, of both strains, as membrane biocatalysts forms mainly beta- and gamma-cyclodextrins after 6 h enzyme reaction at pH 9.0 of the reaction mixture, 35-37% of the reaction products formed are gamma-cyclodextrins Alkalihalobacillus pseudalcaliphilus ?
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Synonyms

Synonyms Comment Organism
cyclodextrin glucanotransferase
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Alkalihalobacillus pseudalcaliphilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
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assay at Alkalihalobacillus pseudalcaliphilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
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assay at Alkalihalobacillus pseudalcaliphilus