Literature summary for 2.4.1.19 extracted from
Atanasova, N.; Kitayska, T.; Yankov, D.; Safarikova, M.; Tonkova, A.
Cyclodextrin glucanotransferase production by cell biocatalysts of alkaliphilic bacilli (2009), Biochem. Eng. J., 46, 278-285.
No PubMed abstract available
Application
Application |
Comment |
Organism |
synthesis |
the enzyme as membrane biocatalyst is applied for a direct cyclodextrin production |
Alkalihalobacillus pseudalcaliphilus |
Protein Variants
Protein Variants |
Comment |
Organism |
additional information |
immobilization of the enzyme by three different techniques: on two types of polysulfone membranes; entrapped in agar-gel beads containing magnetite, and by nano-particles of silanized magnetite covalently bound on the cell surface. The enzyme immobilized on membrane shows highest enzyme activity and high stability, overview |
Alkalihalobacillus pseudalcaliphilus |
General Stability
General Stability |
Organism |
cell membrane-immobilized purified enzyme retains, after 240 h repeated batch cultivation, 1.3-2.3fold increase of the CGTase yield compared to free cells at the end of the process, overview |
Alkalihalobacillus pseudalcaliphilus |
Organism
Organism |
UniProt |
Comment |
Textmining |
Alkalihalobacillus pseudalcaliphilus |
- |
strains 20RF and 8SB, isolated from Bulgarian habitats |
- |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
additional information |
- |
activities of free and immobilized enzymes, overview |
Alkalihalobacillus pseudalcaliphilus |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
additional information |
the immobilized enzyme, of both strains, as membrane biocatalysts forms mainly beta- and gamma-cyclodextrins after 6 h enzyme reaction at pH 9.0 of the reaction mixture, 35-37% of the reaction products formed are gamma-cyclodextrins |
Alkalihalobacillus pseudalcaliphilus |
? |
- |
? |
|
Synonyms
Synonyms |
Comment |
Organism |
cyclodextrin glucanotransferase |
- |
Alkalihalobacillus pseudalcaliphilus |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
55 |
- |
assay at |
Alkalihalobacillus pseudalcaliphilus |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
9 |
- |
assay at |
Alkalihalobacillus pseudalcaliphilus |