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Literature summary for 2.4.1.19 extracted from

  • Li, Z.F.; Li, B.; Liu, Z.G.; Wang, M.; Gu, Z.B.; Du, G.C.; Wu, J.; Chen, J.
    Calcium leads to further increase in glycine-enhanced extracellular secretion of recombinant alpha-cyclodextrin glycosyltransferase in Escherichia coli (2009), J. Agric. Food Chem., 57, 6231-6237.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information synergistic promoting effects of glycine and Ca2+ on the extracellular secretion of the recombinant protein in Escherichia coli, optimal at 150 mM and 20 mM, respectively, overview Paenibacillus macerans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3), glycine, as a medium supplement, can enhance the extracellular secretion of recombinant alpha-CGTase by 11fold. Glycine supplementation exerts impaired cell growth inhibiting an increased enzyme production, but Ca2+ can remedy cell growth inhibition induced by glycine, thereby leading to further increase in the glycine-enhanced extracellular secretion of recombinant alpha-CGTase, effects on the bacterial cell membrane permeability, overview Paenibacillus macerans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ synergistic promoting effects of glycine and Ca2+ on the extracellular secretion of the recombinant protein in Escherichia coli, optimal at 150 mM and 20 mM, respectively, overview Paenibacillus macerans

Organism

Organism UniProt Comment Textmining
Paenibacillus macerans
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-
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Paenibacillus macerans JFB05-01
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows alpha-cyclodextrin forming activity with soluble starch Paenibacillus macerans ?
-
?
additional information the enzyme shows alpha-cyclodextrin forming activity with soluble starch Paenibacillus macerans JFB05-01 ?
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?

Synonyms

Synonyms Comment Organism
alpha-CGTase
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Paenibacillus macerans
alpha-cyclodextrin glycosyltransferase
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Paenibacillus macerans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
assay at Paenibacillus macerans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Paenibacillus macerans