Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | synergistic promoting effects of glycine and Ca2+ on the extracellular secretion of the recombinant protein in Escherichia coli, optimal at 150 mM and 20 mM, respectively, overview | Paenibacillus macerans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3), glycine, as a medium supplement, can enhance the extracellular secretion of recombinant alpha-CGTase by 11fold. Glycine supplementation exerts impaired cell growth inhibiting an increased enzyme production, but Ca2+ can remedy cell growth inhibition induced by glycine, thereby leading to further increase in the glycine-enhanced extracellular secretion of recombinant alpha-CGTase, effects on the bacterial cell membrane permeability, overview | Paenibacillus macerans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | synergistic promoting effects of glycine and Ca2+ on the extracellular secretion of the recombinant protein in Escherichia coli, optimal at 150 mM and 20 mM, respectively, overview | Paenibacillus macerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus macerans | - |
- |
- |
Paenibacillus macerans JFB05-01 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows alpha-cyclodextrin forming activity with soluble starch | Paenibacillus macerans | ? | - |
? | |
additional information | the enzyme shows alpha-cyclodextrin forming activity with soluble starch | Paenibacillus macerans JFB05-01 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-CGTase | - |
Paenibacillus macerans |
alpha-cyclodextrin glycosyltransferase | - |
Paenibacillus macerans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Paenibacillus macerans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Paenibacillus macerans |