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Literature summary for 2.4.1.19 extracted from

  • Bautista, V.; Esclapez, J.; Perez-Pomares, F.; Martinez-Espinosa, R.M.; Camacho, M.; Bonete, M.J.
    Cyclodextrin glycosyltransferase: a key enzyme in the assimilation of starch by the halophilic archaeon Haloferax mediterranei (2012), Extremophiles, 16, 147-159.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NaCl enzyme shows its maximum hydrolysis activity in a buffer with 1.5 M NaCl and retains up to 65% of its activity at 0.5 M NaCl Haloferax mediterranei

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Haloferax mediterranei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km value is 17.2 mg/ml for beta-cyclization activity using soluble starch, pH 7.0, 55°C Haloferax mediterranei

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion by the Tat pathway Haloferax mediterranei
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
x * 82000, SDS-PAGE Haloferax mediterranei

Organism

Organism UniProt Comment Textmining
Haloferax mediterranei Q53I75
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme Haloferax mediterranei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
48
-
pH 7.5, 55°C Haloferax mediterranei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information cyclization is the predominant activity, followed by hydrolysis and to a lesser extent coupling and disproportionation activities Haloferax mediterranei ?
-
?
soluble potato starch
-
Haloferax mediterranei alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin ratio of products is alpha-cyclodextrin to beta-cyclodextrin to gamma-cyclodextrin 1:0.6:0.3 ?

Subunits

Subunits Comment Organism
? x * 82000, SDS-PAGE Haloferax mediterranei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
presence of 1.5 M NaCl Haloferax mediterranei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35
-
50% of maximum activity Haloferax mediterranei
60
-
50% of maximum activity Haloferax mediterranei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
presence of 1.5 M NaCl Haloferax mediterranei

pH Range

pH Minimum pH Maximum Comment Organism
5 8 loss of more than 60% of activity above or below Haloferax mediterranei