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Literature summary for 2.4.1.19 extracted from
- A novel cyclodextrin glycosyltransferase from alkaliphilic Amphibacillus sp. NPST-10: purification and properties (2012), Int. J. Mol. Sci., 13, 10505-10522.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, 87% of initial activity | Salipaludibacillus agaradhaerens |  |
| beta-cyclodextrin | 1 mM, 76% of initial activity | Salipaludibacillus agaradhaerens | |
| Co2+ | 1 mM, 21% of initial activity | Salipaludibacillus agaradhaerens | |
| Cu2+ | 1 mM, 86% of initial activity | Salipaludibacillus agaradhaerens | |
| dithiothreitol | 1 mM, 68% of initial activity | Salipaludibacillus agaradhaerens | |
| EDTA | 1 mM, 67% of initial activity | Salipaludibacillus agaradhaerens | |
| Fe2+ | 1 mM, no residual activity | Salipaludibacillus agaradhaerens | |
| Zn2+ | 1 mM, 29% of initial activity | Salipaludibacillus agaradhaerens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
1 * 85000, SDS-PAGE | Salipaludibacillus agaradhaerens |
| 89000 | - |
gel filtration | Salipaludibacillus agaradhaerens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salipaludibacillus agaradhaerens | - |
- |
- |
| Salipaludibacillus agaradhaerens WN-I | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Salipaludibacillus agaradhaerens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 66.3 | - |
pH 9.0, 55°C | Salipaludibacillus agaradhaerens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Paselli starch | - |
Salipaludibacillus agaradhaerens | beta-cyclodextrin | 78% conversion, 1.9fold higher activity with Paselli starch than with soluble starch | ? | |
| Paselli starch | - |
Salipaludibacillus agaradhaerens WN-I | beta-cyclodextrin | 78% conversion, 1.9fold higher activity with Paselli starch than with soluble starch | ? | |
| soluble starch | 51.5% conversion | Salipaludibacillus agaradhaerens | beta-cyclodextrin | 71% of total cyclodextrin formed | ? | |
| soluble starch | 51.5% conversion | Salipaludibacillus agaradhaerens WN-I | beta-cyclodextrin | 71% of total cyclodextrin formed | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 85000, SDS-PAGE | Salipaludibacillus agaradhaerens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Salipaludibacillus agaradhaerens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
76% of maximum activity | Salipaludibacillus agaradhaerens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal stability is improved in the presence of maltodextrin, starch or CaCl2 | Salipaludibacillus agaradhaerens |
| 40 | - |
stable for at least 1 h | Salipaludibacillus agaradhaerens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Salipaludibacillus agaradhaerens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | stable within | Salipaludibacillus agaradhaerens |
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Release 2023.1 (January 2023)
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