Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus sp. (in: Bacteria) |
Protein Variants | Comment | Organism |
---|---|---|
N132R | introduction of an ionic interaction at the first Ca2+ site, disruption of catalytic activity | Bacillus sp. (in: Bacteria) |
N28R | introduction of an additional ionic interaction at the second Ca2+ site, mutant displays increased cyclization activity | Bacillus sp. (in: Bacteria) |
S182E | mutation adjacent to the first Ca2+ site and the active site cleft. Mutant shows enhanced thermostability, and decreased catalytic activity | Bacillus sp. (in: Bacteria) |
S182G | mutation adjacent to the first Ca2+ site and the active site cleft. Increase in half-life at 60°C to 94 min | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
x * 75000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | Q5U9V9 | - |
- |
Bacillus sp. (in: Bacteria) G1 | Q5U9V9 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.08 | - |
synthesis of alpha-cyclodextrin, 60°C, pH 6.0 | Bacillus sp. (in: Bacteria) |
216 | - |
synthesis of beta-cyclodextrin, 60°C, pH 6.0 | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch | - |
Bacillus sp. (in: Bacteria) | beta-cyclodextrin | main product | ? | |
soluble starch | - |
Bacillus sp. (in: Bacteria) G1 | beta-cyclodextrin | main product | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life 22 min for wild-type, 94 min for mutant S182G, 31 min for mutant S182E | Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
soluble starch | mutant NS182G, pH 6.0, 60°C, calculated as production of beta-cyclodextrin | Bacillus sp. (in: Bacteria) | |
2.6 | - |
soluble starch | mutant S182E, pH 6.0, 60°C, calculated as production of beta-cyclodextrin | Bacillus sp. (in: Bacteria) | |
3.13 | - |
soluble starch | wild-type, pH 6.0, 60°C, calculated as production of beta-cyclodextrin | Bacillus sp. (in: Bacteria) | |
3.5 | - |
soluble starch | mutant N28R, pH 6.0, 60°C, calculated as production of beta-cyclodextrin | Bacillus sp. (in: Bacteria) |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kcat/Km value for wild-type is 1.82 mg/ml, pH 6.0, 60°C | Bacillus sp. (in: Bacteria) |