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Literature summary for 2.4.1.19 extracted from

  • Chen, X.; Huang, L.; Sun, J.; Liu, J.; Wang, W.; Sun, M.; Hao, J.
    Production and characterization of a new alpha-cyclodextrin glycosyltransferase from a marine strain of Bacillus sp. Y112 (2017), J. Biobased Mat. Bioenergy, 11, 236-241 .
No PubMed abstract available
Search for more literature for 2.4.1.19

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 5 mM Bacillus sp. Y112
Al3+ 98% residual activity at 5 mM Bacillus sp. Y112
Ca2+ 93.9% residual activity at 5 mM Bacillus sp. Y112
Co2+ 87.8% residual activity at 5 mM Bacillus sp. Y112
CTAB 98% residual activity at 5 mM Bacillus sp. Y112
Cu2+ 98% residual activity at 5 mM Bacillus sp. Y112
DMSO 98% residual activity at 5 mM Bacillus sp. Y112
EDTA 87.8% residual activity at 5 mM Bacillus sp. Y112
Fe2+ 91.8% residual activity at 5 mM Bacillus sp. Y112
Fe3+ 91.8% residual activity at 5 mM Bacillus sp. Y112
Hg2+ 95.9% residual activity at 5 mM Bacillus sp. Y112
Li+ 95.9% residual activity at 5 mM Bacillus sp. Y112
SDS 81.6% residual activity at 5 mM Bacillus sp. Y112
Tween 20 98% residual activity at 5 mM Bacillus sp. Y112
Zn2+ 85.7% residual activity at 5 mM Bacillus sp. Y112

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzymatic activity increases to 104.1% in the presence of 5 mM MgCl2 Bacillus sp. Y112
Mn2+ the enzymatic activity increases to 104.1% in the presence of 5 mM MnCl2 Bacillus sp. Y112
additional information not influenced by 5 mM Ba2+ Bacillus sp. Y112

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 gel filtration Bacillus sp. Y112

Organism

Organism UniProt Comment Textmining
Bacillus sp. Y112
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ethanol precipitation, Superdex 200 gel filtration and DEAE-Sepharose column chromatography Bacillus sp. Y112

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + glycosyl acceptor
-
 Bacillus sp. Y112 alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 90000, SDS-PAGE Bacillus sp. Y112

Synonyms

Synonyms Comment Organism
alpha-CGTase
-
 Bacillus sp. Y112
alpha-cyclodextrin glycosyltransferase
-
 Bacillus sp. Y112

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Bacillus sp. Y112

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 60 the relative activity at 50°C and 60°C is 93% and 80%, respectively Bacillus sp. Y112

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 45 the purified enzyme is stable up to 45°C after pre-incubation in buffer for 1 h at pH 8.5 Bacillus sp. Y112

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Bacillus sp. Y112

pH Range

pH Minimum pH Maximum Comment Organism
5 10 more than 60% activity between pH 6.0 and 10.0 Bacillus sp. Y112

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 the enzyme retains 100% of its initial activity in a range of pH 7.0 to 9.0 after pre-incubation in buffer for 1 h at 25°C. The enzyme is highly stable at pH 5.0 and 11.0 as indicated by the maintenance of 77% and 72% residual activity, respectively Bacillus sp. Y112