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Literature summary for 2.4.1.2 extracted from

  • Naessens, M.; Cerdobbel, A.; Soetaert, W.; Vandamme, E.J.
    Dextran dextrinase and dextran of Gluconobacter oxydans (2005), J. Ind. Microbiol. Biotechnol., 32, 323-334.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information high enzyme yield when the bacterium is grown on peptone as nitrogen source, and on mannitol or glycerol as carbon source, overview Gluconobacter oxydans

Application

Application Comment Organism
synthesis enzyme is utilized for bioconversions of glycosides in diverse purposes, overview, and for production of dextran and oligodextran, no product inhibition, enzyme is robust in high level production Gluconobacter oxydans

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ partial inhibition at 1 mM Gluconobacter oxydans
Cu2+ partial inhibition at 1 mM Gluconobacter oxydans
Hg2+ complete inactivation at 1 mM Gluconobacter oxydans
KMnO4 complete inactivation at 1 mM Gluconobacter oxydans
Pb2+ complete inactivation at 1 mM Gluconobacter oxydans
Zn2+ partial inhibition at 1 mM Gluconobacter oxydans

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
Gluconobacter oxydans 5618
-
cytoplasm
-
Gluconobacter oxydans 5737
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extracellular cell wall bound Gluconobacter oxydans
-
-
intracellular cytoplasmic Gluconobacter oxydans 5622
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Gluconobacter oxydans substrate specificity, no activity with glucose or maltose, conversion of maltodextrins into (oligo)dextran forming an alpha1,6-linkage, consecutive glucosyl transfer results in formation of high molecular weight dextran, dextran synthesis pathway overview ?
-
?

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
strain ATCC 11894, extra- and intracellular enzyme variants
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Purification (Commentary)

Purification (Comment) Organism
extra- and intracellular enzymes Gluconobacter oxydans

Reaction

Reaction Comment Organism Reaction ID
[(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m = [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1 reaction mode and mechanism Gluconobacter oxydans

Source Tissue

Source Tissue Comment Organism Textmining
additional information high enzyme yield when the bacterium is grown on peptone as nitrogen source, and on mannitol or glycerol as carbon source, overview Gluconobacter oxydans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + starch starch is the donor substrate, cellobiose the acceptor substrate Gluconobacter oxydans ?
-
?
D-glucosyl-alpha-(1,4)-D-xylose + starch starch is the donor substrate, D-glucosyl-alpha(1,4)-D-xylose the acceptor substrate Gluconobacter oxydans ?
-
?
dextran + salicin dextran is the donor substrate, salicin the acceptor substrate Gluconobacter oxydans ?
-
?
gentiobiose + starch starch is the donor substrate, gentiobiose the acceptor substrate Gluconobacter oxydans ?
-
?
isomaltose + salicin isomaltose is the donor substrate, salicin the acceptor substrate Gluconobacter oxydans ?
-
?
isomaltose + starch starch is the donor substrate, isomaltose the acceptor substrate Gluconobacter oxydans ?
-
?
isoprimeverose + starch starch is the donor substrate, isoprimeverose the acceptor substrate Gluconobacter oxydans ?
-
?
kojibiose + starch starch is the donor substrate, kojibiose the acceptor substrate Gluconobacter oxydans ?
-
?
laminaribiose + starch starch is the donor substrate, laminaribiose the acceptor substrate Gluconobacter oxydans ?
-
?
maltose + salicin maltose is the donor substrate, salicin the acceptor substrate Gluconobacter oxydans ?
-
?
maltose + starch starch is the donor substrate, maltose the acceptor substrate Gluconobacter oxydans ?
-
?
methyl-alpha-D-glucoside + starch starch is the donor substrate, methyl-alpha-D-glucoside the acceptor substrate Gluconobacter oxydans methyl-alpha-isomaltoside + ?
-
?
methyl-beta-D-glucoside + starch starch is the donor substrate, methyl-beta-D-glucoside the acceptor substrate Gluconobacter oxydans methyl-beta-isomaltoside + ?
-
?
additional information substrate specificity, no activity with glucose or maltose, conversion of maltodextrins into (oligo)dextran forming an alpha1,6-linkage, consecutive glucosyl transfer results in formation of high molecular weight dextran, dextran synthesis pathway overview Gluconobacter oxydans ?
-
?
additional information donor and acceptor substrate specificity, no activity with trehalose, lactose, or melibiose as acceptor substrates, when starch is the donor substrate, overview, conversion of maltodextrins into (oligo)dextran forming an alpha1,6-linkage or an alpha1,4-linkage Gluconobacter oxydans ?
-
?
nigerose + starch starch is the donor substrate, nigerose the acceptor substrate Gluconobacter oxydans ?
-
?
salicin + starch starch is the donor substrate, salicin, i.e. 2-(hydroxymethyl)phenyl-beta-D-glucopyranoside, the acceptor substrate Gluconobacter oxydans ?
-
?
sophorose + starch starch is the donor substrate, sophorose the acceptor substrate Gluconobacter oxydans ?
-
?
starch + salicin starch is the donor substrate, salicin the acceptor substrate Gluconobacter oxydans ?
-
?
sucrose + starch starch is the donor substrate, sucrose the acceptor substrate Gluconobacter oxydans ?
-
?
xylosucrose + starch starch is the donor substrate, xylosucrose the acceptor substrate Gluconobacter oxydans ?
-
?

Synonyms

Synonyms Comment Organism
DDase
-
Gluconobacter oxydans
dextran dextrinase
-
Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
Gluconobacter oxydans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
purified extracellular enzyme, stable up to Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
Gluconobacter oxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.1 5.4 purified extracellular enzyme, stable for 24 h at 4°C Gluconobacter oxydans