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Literature summary for 2.4.1.20 extracted from
- Characterization of a cellobiose phosphorylase from a hyperthermophilic eubacterium, Thermotoga maritima MSB8 (2002), Biosci. Biotechnol. Biochem., 66, 2578-2586.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Thermotoga maritima |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | substrate inhibition, competitive with glucose-1-phosphate | Thermotoga maritima |  |
| D-glucose 1-phosphate | - |
Thermotoga maritima | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
phosphate | - |
Thermotoga maritima | |
| 0.29 | - |
cellobiose | - |
Thermotoga maritima | |
| 0.69 | - |
D-glucose | - |
Thermotoga maritima | |
| 4.1 | - |
6-deoxy-D-glucose | - |
Thermotoga maritima | |
| 5.7 | - |
D-glucosamine | - |
Thermotoga maritima | |
| 14 | - |
D-xylose | - |
Thermotoga maritima | |
| 47 | - |
2-deoxy-D-glucose | - |
Thermotoga maritima | |
| 67 | - |
D-mannose | - |
Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
SDS-PAGE | Thermotoga maritima |
| 94320 | - |
calculated from DNA-sequence | Thermotoga maritima |
| 150000 | - |
gel filtration | Thermotoga maritima |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + phosphate | Thermotoga maritima | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
| cellobiose + phosphate | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
| Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga maritima |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.66 | - |
purified enzyme | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose | - |
Thermotoga maritima | ? | - |
? | |
| alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
| alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose | - |
Thermotoga maritima | ? | - |
? | |
| alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
| alpha-D-glucose 1-phosphate + D-glucosamine | - |
Thermotoga maritima | ? | - |
? | |
| alpha-D-glucose 1-phosphate + D-mannose | - |
Thermotoga maritima | ? | - |
? | |
| alpha-D-glucose 1-phosphate + D-xylose | no activity with L-isomer | Thermotoga maritima | ? | - |
? | |
| alpha-D-glucose 1-phosphate + D-xylose | higher specific activity than with D-glucose as acceptor | Thermotoga maritima | ? | - |
? | |
| cellobiose + phosphate | - |
Thermotoga maritima | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
| cellobiose + phosphate | random ordered bi-bi mechanism | Thermotoga maritima | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
| cellobiose + phosphate | sequential bi-bi mechanism | Thermotoga maritima | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
| cellobiose + phosphate | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
| cellobiose + phosphate | random ordered bi-bi mechanism | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
| cellobiose + phosphate | sequential bi-bi mechanism | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 85000, gel filtration, SDS-PAGE | Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Thermotoga maritima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 95 | - |
Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
less than 10% loss of activity after heating for 30 min at pH 6-8 | Thermotoga maritima |
| 90 | - |
25% loss of activity after heating for 30 min at pH 6-8 | Thermotoga maritima |
| 100 | - |
complete loss of activity after heating for 30 min at pH 6-8 | Thermotoga maritima |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.4 | - |
D-mannose | - |
Thermotoga maritima | |
| 5.2 | - |
D-glucosamine | - |
Thermotoga maritima | |
| 5.4 | - |
cellobiose | - |
Thermotoga maritima | |
| 8 | - |
D-glucose | - |
Thermotoga maritima | |
| 16 | - |
2-deoxy-D-glucose | - |
Thermotoga maritima | |
| 17 | - |
6-deoxy-D-glucose | - |
Thermotoga maritima | |
| 40 | - |
D-xylose | - |
Thermotoga maritima | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
- |
Thermotoga maritima |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | - |
Thermotoga maritima |
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