Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.207 extracted from

  • Schröder, R.; Atkinson, R.G.; Langenkämper, G.; Redgwell, R.J.
    Biochemical and molecular characterisation of xyloglucan endotransglycosylase from ripe kiwifruit (1998), Planta, 204, 242-251.
    View publication on PubMed
Search for more literature for 2.4.1.207

Cloned(Commentary)

Cloned (Comment) Organism
6 isoforms, AdXET1-6 gene family is cloned, a full-length cDNA, AdXET5, is cloned, sequenced and overexpressed as active XET in Escherichia coli, purified XET is encoded by AdXET6 Actinidia deliciosa

General Stability

General Stability Organism
loss of activity if frozen and stored at -80°C Actinidia deliciosa

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM Actinidia deliciosa
Hg2+ at least 10 mM Actinidia deliciosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km: 0.6 mg/ml for kiwifruit xyloglucan Actinidia deliciosa
0.1
-
 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol reduced xyloglucan-derived heptasaccharide XXXG-ol, derived from kiwifruit xyloglucan Actinidia deliciosa

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall most of the XET activity is strongly associated with the cell wall Actinidia deliciosa 5618
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 x * 34000, predicted MW of the mature protein encoded by AdXET5 is 31.8 kDa, SDS-PAGE Actinidia deliciosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
donor xyloglucan + xyloglucan acceptor Actinidia deliciosa XET and xyloglucan may play a role in the cell wall changes that accompany fruit softening during ripening, substrates for XET action are located in the cell wall ?
-
 ?

Organism

Organism UniProt Comment Textmining
Actinidia deliciosa
-
 ripe kiwifruit, [A. Chev.] C.F. Liang et A.R. Ferguson var. deliciosa cv. Hayward, 6 isoenzymes: AdXET1-6 gene family
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein AdXET5 and AdXET6: asparagine-linked carbohydrate, N-glycosylated, glycosylation of the protein is not required for endo-transglycosylase activity Actinidia deliciosa
proteolytic modification 21 amino acids signal peptide at the N-terminus is cleaved to produce the mature protein Actinidia deliciosa

Purification (Commentary)

Purification (Comment) Organism
3000fold, AdXET6 Actinidia deliciosa

Source Tissue

Source Tissue Comment Organism Textmining
fruit core tissue of ripe kiwifruit Actinidia deliciosa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Actinidia deliciosa

Storage Stability

Storage Stability Organism
-80°C, partially purified XET, ConA-eluate stage, 4 weeks, stable Actinidia deliciosa
-80°C, purified XET, loss of activity Actinidia deliciosa
4°C, purified XET, 24 h, stable Actinidia deliciosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
donor xyloglucan + acceptor xyloglucan kiwifruit xyloglucan donor, acceptor: XXXG-ol, a reduced heptasaccharide derived from kiwifruit xyloglucan Actinidia deliciosa ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol XXXG-ol Actinidia deliciosa ?
-
 ?
donor xyloglucan + xyloglucan acceptor XET and xyloglucan may play a role in the cell wall changes that accompany fruit softening during ripening, substrates for XET action are located in the cell wall Actinidia deliciosa ?
-
 ?
additional information in absence of reduced xyloglucan-derived heptasaccharide XXXG-ol enzyme depolymerizes xyloglucan by hydrolysis and in the presence of it by both hydrolysis and endotransglycosylation Actinidia deliciosa ?
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, predicted MW of the mature protein encoded by AdXET5 is 31.8 kDa, SDS-PAGE Actinidia deliciosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Actinidia deliciosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 5.8
-
 Actinidia deliciosa

pH Range

pH Minimum pH Maximum Comment Organism
5 8 active between Actinidia deliciosa