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Literature summary for 2.4.1.207 extracted from

  • Steele, N.M.; Sulova, Z.; Campbell, P.; Braam, J.; Farkas, V.; Fry, S.C.
    Ten isoenzymes of xyloglucan endotransglycosylase from plant cell walls select and cleave the donor substrate stochastically (2001), Biochem. J., 355, 671-679.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.207

Cloned(Commentary)

Cloned (Comment) Organism
3 insect-cell-produced XETs: EXGT, TCH4 and MERI-5 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
donor xyloglucan + xyloglucan acceptor Arabidopsis thaliana Arabidopsis MERI-5 and TCH4 and cauliflower isoenzymes expression occur in dense cytoplasmic tissues predominantly involved in the assembly of new cell walls and thus in the integration of newly secreted xyloglucan into the cell wall ?
-
 ?
donor xyloglucan + xyloglucan acceptor Brassica oleracea Arabidopsis MERI-5 and TCH4 and cauliflower isoenzymes expression occur in dense cytoplasmic tissues predominantly involved in the assembly of new cell walls and thus in the integration of newly secreted xyloglucan into the cell wall ?
-
 ?
donor xyloglucan + xyloglucan acceptor Vigna radiata var. radiata Arabidopsis EXGT and mung bean isoenzymes expression in tissues involved in the loosening of existing cell wall material necessary for rapid cell expansion, e.g. vacuolation ?
-
 ?
donor xyloglucan + xyloglucan acceptor Arabidopsis thaliana Arabidopsis EXGT and mung bean isoenzymes expression in tissues involved in the loosening of existing cell wall material necessary for rapid cell expansion, e.g. vacuolation ?
-
 ?
donor xyloglucan + xyloglucan acceptor Lens culinaris lentil and nasturtium, NXG1, seed enzymes are involved in the mobilization of cotyledonary xyloglucan reserves after germination ?
-
 ?
donor xyloglucan + xyloglucan acceptor Tropaeolum majus lentil and nasturtium, NXG1, seed enzymes are involved in the mobilization of cotyledonary xyloglucan reserves after germination ?
-
 ?
additional information Vigna radiata var. radiata free oligosaccharides are probably not the usual acceptor substrates in vivo ?
-
 ?
additional information Arabidopsis thaliana free oligosaccharides are probably not the usual acceptor substrates in vivo ?
-
 ?
additional information Lens culinaris free oligosaccharides are probably not the usual acceptor substrates in vivo ?
-
 ?
additional information Brassica oleracea free oligosaccharides are probably not the usual acceptor substrates in vivo ?
-
 ?
additional information Tropaeolum majus free oligosaccharides are probably not the usual acceptor substrates in vivo ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 3 insect-cell-produced XETs: EXGT, TCH4 and MERI-5, EXGT belongs to XET class I, TCH4 and MERI-5 to XET class II
-
Brassica oleracea
-
 cauliflower
-
Brassica oleracea
-
 isoenzyme C45a
-
Lens culinaris
-
-
-
Tropaeolum majus
-
 NXG1 is a member of XET class III
-
Vigna radiata var. radiata
-
 4 isoenzymes: M35, M45, M55a, M55b
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vigna radiata var. radiata
-
 Lens culinaris
-
 Brassica oleracea
-
 Tropaeolum majus
TCH4 and EXGT Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
floret
-
 Brassica oleracea
-
seed
-
 Lens culinaris
-
seed
-
 Tropaeolum majus
-
shoot
-
 Arabidopsis thaliana
-
shoot etiolated shoots Vigna radiata var. radiata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Vigna radiata var. radiata ?
-
 ?
donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Arabidopsis thaliana ?
-
 ?
donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Lens culinaris ?
-
 ?
donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Brassica oleracea ?
-
 ?
donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Tropaeolum majus ?
-
 ?
donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Vigna radiata var. radiata ?
-
 ?
donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Arabidopsis thaliana ?
-
 ?
donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Lens culinaris ?
-
 ?
donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Brassica oleracea ?
-
 ?
donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Tropaeolum majus ?
-
 ?
donor xyloglucan + acceptor xyloglucan enzyme chooses its donor substrate independently of size and attacks it, once only, at a randomly selected cleavage site Vigna radiata var. radiata ?
-
 ?
donor xyloglucan + acceptor xyloglucan enzyme chooses its donor substrate independently of size and attacks it, once only, at a randomly selected cleavage site Arabidopsis thaliana ?
-
 ?
donor xyloglucan + acceptor xyloglucan enzyme chooses its donor substrate independently of size and attacks it, once only, at a randomly selected cleavage site Lens culinaris ?
-
 ?
donor xyloglucan + acceptor xyloglucan enzyme chooses its donor substrate independently of size and attacks it, once only, at a randomly selected cleavage site Brassica oleracea ?
-
 ?
donor xyloglucan + acceptor xyloglucan enzyme chooses its donor substrate independently of size and attacks it, once only, at a randomly selected cleavage site Tropaeolum majus ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol comparison of the size distributions of the different isoenzymes’ transglycosylation products Vigna radiata var. radiata ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol comparison of the size distributions of the different isoenzymes’ transglycosylation products Arabidopsis thaliana ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol comparison of the size distributions of the different isoenzymes’ transglycosylation products Lens culinaris ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol comparison of the size distributions of the different isoenzymes’ transglycosylation products Brassica oleracea ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol comparison of the size distributions of the different isoenzymes’ transglycosylation products Tropaeolum majus ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol isoenzyme C45a dissociates from the reaction products after each polysaccharide-to-oligosaccharide transglycosylation event Brassica oleracea ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol donor: tamarind xyloglucan Vigna radiata var. radiata ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol donor: tamarind xyloglucan Arabidopsis thaliana ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol donor: tamarind xyloglucan Lens culinaris ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol donor: tamarind xyloglucan Brassica oleracea ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol donor: tamarind xyloglucan Tropaeolum majus ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol XLLG-ol Vigna radiata var. radiata ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol XLLG-ol Arabidopsis thaliana ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol XLLG-ol Lens culinaris ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol XLLG-ol Brassica oleracea ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol XLLG-ol Tropaeolum majus ?
-
 ?
donor xyloglucan + xyloglucan acceptor Arabidopsis MERI-5 and TCH4 and cauliflower isoenzymes expression occur in dense cytoplasmic tissues predominantly involved in the assembly of new cell walls and thus in the integration of newly secreted xyloglucan into the cell wall Arabidopsis thaliana ?
-
 ?
donor xyloglucan + xyloglucan acceptor Arabidopsis MERI-5 and TCH4 and cauliflower isoenzymes expression occur in dense cytoplasmic tissues predominantly involved in the assembly of new cell walls and thus in the integration of newly secreted xyloglucan into the cell wall Brassica oleracea ?
-
 ?
donor xyloglucan + xyloglucan acceptor Arabidopsis EXGT and mung bean isoenzymes expression in tissues involved in the loosening of existing cell wall material necessary for rapid cell expansion, e.g. vacuolation Vigna radiata var. radiata ?
-
 ?
donor xyloglucan + xyloglucan acceptor Arabidopsis EXGT and mung bean isoenzymes expression in tissues involved in the loosening of existing cell wall material necessary for rapid cell expansion, e.g. vacuolation Arabidopsis thaliana ?
-
 ?
donor xyloglucan + xyloglucan acceptor lentil and nasturtium, NXG1, seed enzymes are involved in the mobilization of cotyledonary xyloglucan reserves after germination Lens culinaris ?
-
 ?
donor xyloglucan + xyloglucan acceptor lentil and nasturtium, NXG1, seed enzymes are involved in the mobilization of cotyledonary xyloglucan reserves after germination Tropaeolum majus ?
-
 ?
additional information free oligosaccharides are probably not the usual acceptor substrates in vivo Vigna radiata var. radiata ?
-
 ?
additional information free oligosaccharides are probably not the usual acceptor substrates in vivo Arabidopsis thaliana ?
-
 ?
additional information free oligosaccharides are probably not the usual acceptor substrates in vivo Lens culinaris ?
-
 ?
additional information free oligosaccharides are probably not the usual acceptor substrates in vivo Brassica oleracea ?
-
 ?
additional information free oligosaccharides are probably not the usual acceptor substrates in vivo Tropaeolum majus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Vigna radiata var. radiata
20
-
 assay at Arabidopsis thaliana
20
-
 assay at Lens culinaris
20
-
 assay at Brassica oleracea
20
-
 assay at Tropaeolum majus