Cloned (Comment) | Organism |
---|---|
NtXET-1 is cloned and expressed in Escherichia coli, ORF encodes a 295 amino acids protein, cDNA is cloned in sense and antisense orientation, creation of transgenic tobacco plants with reduced NtXET-1 expression: 2 independent lines with reduced total XET activity by 56% and 37%, respectively, in midribs of tobacco plants transformed with an antisense construct, which exhibit an at least 20% increase in the average MW of xyloglucan | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | the same gene encodes a protein, that may be both soluble and bound to the cell wall | Nicotiana tabacum | 5618 | - |
soluble | the same gene encodes a protein, that may be both soluble and bound to the cell wall | Nicotiana tabacum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33900 | - |
x * 33900, calculated from the amino acid sequence | Nicotiana tabacum |
36000 | - |
x * 36000, probably glycosylated enzyme, Western blot | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
donor xyloglucan + xyloglucan acceptor | Nicotiana tabacum | NtXET-1 is involved in the incorporation of small xyloglucan molecules into the cell wall by transglycosylation, role during differentation and growth of the vascular tissue, reduced NtXET-1 expression and increase in the MW of xyloglucans in older leaves might be associated with strengthening of cell walls by reduced turnover and hydrolysis of xyloglucan, control of NtXET-1 expression in leaves | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | P93349 | cv. Samsun NN, NtXET-1, belongs to group I of XET-related subfamilies | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | two putative glycosylation sites at amino acid positions 14 and 114 | Nicotiana tabacum |
proteolytic modification | contains a hydrophobic N-terminus indicating a signal peptide | Nicotiana tabacum |
Purification (Comment) | Organism |
---|---|
recombinant NtXET-1, expressed in Escherichia coli | Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | source leaf, levels of NtXET-1 mRNA decreases in midribs with increasing age of leaves | Nicotiana tabacum | - |
additional information | tissue-specific NtXET-1 expression pattern, highest mRNA levels in organs highly enriched in vascular tissues | Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
donor xyloglucan + acceptor xyloglucan | NtXET-1 has a prefence for smaller xyloglucan molecules as acceptors | Nicotiana tabacum | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | donor: pine or tobacco xyloglucan | Nicotiana tabacum | ? | - |
? | |
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol | pine or tobacco xyloglucan as donor | Nicotiana tabacum | ? | - |
? | |
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol | XXXG-ol | Nicotiana tabacum | ? | - |
? | |
donor xyloglucan + xyloglucan acceptor | NtXET-1 is involved in the incorporation of small xyloglucan molecules into the cell wall by transglycosylation, role during differentation and growth of the vascular tissue, reduced NtXET-1 expression and increase in the MW of xyloglucans in older leaves might be associated with strengthening of cell walls by reduced turnover and hydrolysis of xyloglucan, control of NtXET-1 expression in leaves | Nicotiana tabacum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33900, calculated from the amino acid sequence | Nicotiana tabacum |
? | x * 36000, probably glycosylated enzyme, Western blot | Nicotiana tabacum |