KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
rhodamin-conjugated Glc3-Xyl3-Gal2-glucitol | isozyme eXET, pH 5.5, 30°C | Tropaeolum majus | |
0.131 | - |
rhodamin-conjugated Glc3-Xyl3-Gal2-glucitol | isozyme sXET, pH 5.5, 30°C | Tropaeolum majus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tropaeolum majus | - |
2 isoenzymes, one of which is seed specific | - |
Purification (Comment) | Organism |
---|---|
isozymes sXET and eXET from germinated seeds, i.e. cotyledons, and from epicotyls, respectively, to near homogeneity by ammonium sulfate fractionation, anion exchange chromatography, adsorption and desorption of the enzyme-substrate complex on cellulose, and hydrophobic-interaction chromatography | Tropaeolum majus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc | determination of cleavage sites within the donor substrate of isozymes | Tropaeolum majus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epicotyl | isozyme eXET | Tropaeolum majus | - |
additional information | isozyme eXET also occurs in other growing regions | Tropaeolum majus | - |
seed | expression of isozyme sXET during germination | Tropaeolum majus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | isozymes sXET and eXET show different specificities for xyloglucan-derived oligosaccharide acceptor substrates, and distinct preferences in selecting the site of attack on xyloglucan molecules | Tropaeolum majus | ? | - |
? | |
rhodamin-conjugated Glc3-Xyl3-Gal2-glucitol + xyloglucan | acceptor substrate: rhodamin-conjugated reduced xyloglucan-derived nonasaccharide, donor substrate: tamarind-seed xyloglucan, fluorescence assay | Tropaeolum majus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XET | - |
Tropaeolum majus |
xyloglucan endo-transglycosylase | - |
Tropaeolum majus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Tropaeolum majus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | isozyme eXET | Tropaeolum majus |
5.5 | 8 | isozyme sXET, broad optimum | Tropaeolum majus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
isozymes sXET and eXET show different pH-profiles | Tropaeolum majus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Tropaeolum majus | about, isozymes sXET and eXET, isoelectric focusing | - |
6.6 |