Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acidic arabinogalactan protein | MW about 5000 Da, acidic polymers and/or other apoplastic ions are naturally occurring regulators of the enzyme activity in vivo, and may thus control cell wall assembly, loosening, and growth, composition overview | Brassica oleracea | |
Gum arabic | from Acacia sp., 0.02-0.4% w/v, activation of desalted enzyme, activation is increased in presence of suboptimal concentration of NaCl | Brassica oleracea | |
hypochlorite-oxidized xyloglucan | from tamarind, 0.02-0.4% w/v, activation of desalted enzyme, activation is increased in presence of suboptimal concentration of NaCl | Brassica oleracea | |
additional information | no activation by carboxymethylcellulose, non-oxidized xyloglucan, homo-galacturonic acid, and starch, overview | Brassica oleracea | |
Pectin | from Citrus sp., 0.02-0.4% w/v, activation of desalted enzyme, activation is increased in presence of suboptimal concentration of NaCl | Brassica oleracea |
General Stability | Organism |
---|---|
activity is lost after partial purification and desalting, ascorbic acid and 2-mercaptoethanol cannot prevent the loss, activity can be restored by addition of a wide variety of inorganic and organic salts | Brassica oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-O-methyl-glucuronoxylan | - |
Brassica oleracea | |
AlCl3 | inhibitory at concentrations above 0.1 mM | Brassica oleracea | |
alginate | alpha-carrageenan from red algae Gigartina ssp. | Brassica oleracea | |
CaCl2 | inhibitory at concentrations above 0.1 mM | Brassica oleracea | |
homogalacturonan | - |
Brassica oleracea | |
LaCl3 | inhibitory at 5 mM | Brassica oleracea | |
MgCl2 | inhibitory at concentrations above 0.1 mM | Brassica oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Brassica oleracea | 5618 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Al3+ | best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations | Brassica oleracea | |
Ca2+ | best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations | Brassica oleracea | |
K+ | best at about 300 mM | Brassica oleracea | |
La3+ | best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations | Brassica oleracea | |
Mg2+ | best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations | Brassica oleracea | |
additional information | the enzyme is activated by a wide variety of inorganic and organic salts | Brassica oleracea | |
Na+ | best at about 300 mM | Brassica oleracea | |
NH4+ | best at about 300 mM | Brassica oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Brassica oleracea | acidic polymers, probably acidic arabinogalactan proteins, and/or other apoplastic ions are naturally occurring regulators of the enzyme activity in vivo, and may thus control cell wall assembly, loosening, and growth | ? | - |
? | |
xyloglucan oligosaccharides + xyloglucan | Brassica oleracea | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica oleracea | - |
cauliflower | - |
Purification (Comment) | Organism |
---|---|
partial | Brassica oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
floret | - |
Brassica oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Brassica oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | acidic polymers, probably acidic arabinogalactan proteins, and/or other apoplastic ions are naturally occurring regulators of the enzyme activity in vivo, and may thus control cell wall assembly, loosening, and growth | Brassica oleracea | ? | - |
? | |
xyloglucan oligosaccharides + xyloglucan | - |
Brassica oleracea | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XET | - |
Brassica oleracea |
xyloglucan endotransglucosylase | - |
Brassica oleracea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
assay at | Brassica oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Brassica oleracea |