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Literature summary for 2.4.1.207 extracted from

  • Takeda, T.; Fry, S.C.
    Control of xyloglucan endotransglucosylase activity by salts and anionic polymers (2004), Planta, 219, 722-732.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
acidic arabinogalactan protein MW about 5000 Da, acidic polymers and/or other apoplastic ions are naturally occurring regulators of the enzyme activity in vivo, and may thus control cell wall assembly, loosening, and growth, composition overview Brassica oleracea
Gum arabic from Acacia sp., 0.02-0.4% w/v, activation of desalted enzyme, activation is increased in presence of suboptimal concentration of NaCl Brassica oleracea
hypochlorite-oxidized xyloglucan from tamarind, 0.02-0.4% w/v, activation of desalted enzyme, activation is increased in presence of suboptimal concentration of NaCl Brassica oleracea
additional information no activation by carboxymethylcellulose, non-oxidized xyloglucan, homo-galacturonic acid, and starch, overview Brassica oleracea
Pectin from Citrus sp., 0.02-0.4% w/v, activation of desalted enzyme, activation is increased in presence of suboptimal concentration of NaCl Brassica oleracea

General Stability

General Stability Organism
activity is lost after partial purification and desalting, ascorbic acid and 2-mercaptoethanol cannot prevent the loss, activity can be restored by addition of a wide variety of inorganic and organic salts Brassica oleracea

Inhibitors

Inhibitors Comment Organism Structure
4-O-methyl-glucuronoxylan
-
Brassica oleracea
AlCl3 inhibitory at concentrations above 0.1 mM Brassica oleracea
alginate alpha-carrageenan from red algae Gigartina ssp. Brassica oleracea
CaCl2 inhibitory at concentrations above 0.1 mM Brassica oleracea
homogalacturonan
-
Brassica oleracea
LaCl3 inhibitory at 5 mM Brassica oleracea
MgCl2 inhibitory at concentrations above 0.1 mM Brassica oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
Brassica oleracea 5618
-

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations Brassica oleracea
Ca2+ best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations Brassica oleracea
K+ best at about 300 mM Brassica oleracea
La3+ best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations Brassica oleracea
Mg2+ best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations Brassica oleracea
additional information the enzyme is activated by a wide variety of inorganic and organic salts Brassica oleracea
Na+ best at about 300 mM Brassica oleracea
NH4+ best at about 300 mM Brassica oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Brassica oleracea acidic polymers, probably acidic arabinogalactan proteins, and/or other apoplastic ions are naturally occurring regulators of the enzyme activity in vivo, and may thus control cell wall assembly, loosening, and growth ?
-
?
xyloglucan oligosaccharides + xyloglucan Brassica oleracea
-
?
-
?

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
cauliflower
-

Purification (Commentary)

Purification (Comment) Organism
partial Brassica oleracea

Source Tissue

Source Tissue Comment Organism Textmining
floret
-
Brassica oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Brassica oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information acidic polymers, probably acidic arabinogalactan proteins, and/or other apoplastic ions are naturally occurring regulators of the enzyme activity in vivo, and may thus control cell wall assembly, loosening, and growth Brassica oleracea ?
-
?
xyloglucan oligosaccharides + xyloglucan
-
Brassica oleracea ?
-
?

Synonyms

Synonyms Comment Organism
XET
-
Brassica oleracea
xyloglucan endotransglucosylase
-
Brassica oleracea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
assay at Brassica oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
assay at Brassica oleracea