Any feedback?
Literature summary for 2.4.1.207 extracted from
- Enzymic characterization of two recombinant xyloglucan endotransglucosylase/hydrolase (XTH) proteins of Arabidopsis and their effect on root growth and cell wall extension (2009), J. Exp. Bot., 60, 3959-3972.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain GS115 | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Hi-Trap SP Sepharose column chromatography, Superdex 75 gel filtration | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | isozymes XTH14 and XTH26 are predominantly root-specific | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| donor tamarind xyloglucan + acceptor xyloglucan | isozymes XTH14 and XTH26 show very high preference for xyloglucan as donor substrate, yet do not work exclusively on xyloglucan, there is a clear preference for the octasaccharide XXLGol, whereas XLLGol is the second best (tested) substrate for isozyme XTH14, it seems a rather poor substrate for isozyme XTH26, which prefers XXFGol | Arabidopsis thaliana | ? | - |
? |  |
| additional information | isozyme XTH14 uses donor substrates in order of preference xyloglucan >> watersoluble cellulose acetate > hydroxyethylcellulose > mixed-linkage beta-glucan > carboxymethylcellulose > methylcellulose, thus, all nonxyloglucan substrates show very low activity rates in comparison to xyloglucan. Isozyme XTH26 is able to use watersoluble cellulose acetate at a low rate but shows no activity with any of the other non-xyloglucan substrates tested | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XET | - |
Arabidopsis thaliana |
| XTH | - |
Arabidopsis thaliana |
| XTH14 | isozyme | Arabidopsis thaliana |
| XTH26 | isozyme | Arabidopsis thaliana |
| xyloglucan endotransglucosylase/hydrolase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Arabidopsis thaliana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
37 | isozyme XTH14 shows high activity at 37°C, and at least 80% of this activity is maintained down to 20°C. At 4°C the protein still displays more than 60% of ist activity at 37°C. The activity abruptly decreases to about 30% at 0°C. Isozyme XTH26 shows high activity at 37°C, and at least 50% of this activity is maintained down to 20°C. At 4°C the protein still displays more than 30% of ist activity at 37°C, while the activity abruptly decreases to about 15% at 0°C | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
isozyme XTH26 | Arabidopsis thaliana |
| 6.5 | - |
isozyme XTH14 | Arabidopsis thaliana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | both isoenzymes show a fast and symmetrical decrease in activity on both sides of their optima followed by an acidic shoulder between pH 4.0 and 5.0 for XTH14 and between pH 4.0 and 4.5 for XTH26. At pH 4.0 and 8.0, XTH14 retains about 30% and XTH26 40% of its maximal activity | Arabidopsis thaliana |
| 5 | 7 | isozyme XTH26 | Arabidopsis thaliana |
| 5.5 | 7 | isozyme XTH14 | Arabidopsis thaliana |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
