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Literature summary for 2.4.1.207 extracted from

  • Stratilova, E.; Ait-Mohand, F.; Rehulka, P.; Garajova, S.; Flodrova, D.; Rehulkova, H.; Farkas, V.
    Xyloglucan endotransglycosylases (XETs) from germinating nasturtium (Tropaeolum majus) seeds: Isolation and characterization of the major form (2010), Plant Physiol. Biochem., 48, 207-215.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
xyloglucan-derived oligosaccharide
-
Tropaeolum majus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26500
-
deglycosylated major enzyme form XET(6.3), SDS-PAGE Tropaeolum majus
29000
-
glycosylated major enzyme form XET(6.3), SDS-PAGE Tropaeolum majus

Organism

Organism UniProt Comment Textmining
Tropaeolum majus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Tropaeolum majus

Purification (Commentary)

Purification (Comment) Organism
polyanion SI column chromatography, Spheron phosphate column chromatography, and Superdex 75 gel filtration Tropaeolum majus

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Tropaeolum majus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
donor xyloglucan + acceptor beta-(1,4)-xylooligosaccharide the transglycosylating activity is 0.31% of that determined with xyloglucan oligosaccharide as acceptor Tropaeolum majus ?
-
?
donor xyloglucan + acceptor beta-(1,6)-D-gluco-oligosaccharide the transglycosylating activity is 0.69% of that determined with xyloglucan oligosaccharide as acceptor Tropaeolum majus ?
-
?
donor xyloglucan + acceptor cellooligosaccharide the transglycosylating activity is 4.6% of that determined with xyloglucan oligosaccharide as acceptor Tropaeolum majus ?
-
?
donor xyloglucan + acceptor laminarioligosaccharide the transglycosylating activity is 0.23% of that determined with xyloglucan oligosaccharide as acceptor Tropaeolum majus ?
-
?
donor xyloglucan + acceptor mixed-linkage beta-(1,3 1,4)-gluco-oligosaccharide the transglycosylating activity is 2.06% of that determined with xyloglucan oligosaccharide as acceptor Tropaeolum majus ?
-
?
donor xyloglucan + acceptor pustulooligosaccharide
-
Tropaeolum majus ?
-
?
donor xyloglucan + acceptor xyloglucan
-
Tropaeolum majus ?
-
?
additional information the enzyme exhibits broad substrate specificity by transferring xyloglucan or hydroxyethylcellulose fragments not only to oligoxyloglucosides and cellooligosaccharides but also to oligosaccharides derived from beta-(1,4)-D-glucuronoxylan, beta-(1,6)-D-glucan, mixed-linkage beta-(1,3 1,4)-D-glucan and at a relatively low rate also to beta-(1,3)-gluco-oligosaccharides Tropaeolum majus ?
-
?

Synonyms

Synonyms Comment Organism
XET(6.3) major enzyme form, XET(6.3) is classified into group II of the XTH phylogeny of glycoside hydrolase family GH16 Tropaeolum majus
XTH
-
Tropaeolum majus
xyloglucan endotransglycosylase
-
Tropaeolum majus
xyloglucan endotransglycosylase/hydrolase
-
Tropaeolum majus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
Tropaeolum majus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
38 50 the activity of XETs from nasturtium seeds is stable at 40°C during 2 h (at 38°C during 8 h), but it is completely lost at 50°C Tropaeolum majus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 8.5
-
Tropaeolum majus

pI Value

Organism Comment pI Value Maximum pI Value
Tropaeolum majus major enzyme form XET(6.3), isoelectric focusing
-
6.3