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Literature summary for 2.4.1.207 extracted from

  • Valenzuela, C.; Ramos, P.; Carrasco, C.; Moya-Leon, M.; Herrera, R.
    Cloning and characterization of a xyloglucan endo-transglycosylase/hydrolase gene expressed in response to inclination in radiata pine seedlings (2014), Tree Genet. Genomes, 10, 1305-1315.
No PubMed abstract available
Search for more literature for 2.4.1.207

Cloned(Commentary)

Cloned (Comment) Organism
gene PrXTH1, DNA and amino acid sequence determination and analyyis, phylogenetic analysis, gene PrXTH1 belongs to group I of XTHs. The recombinant purified protein has only transglycosylase activity. Quantitative real time-PCR enzyme expression analysis. Recombinant expression of C-terminally HIs-tagged enzyme in Pichia pastoris strain X-33 Pinus radiata

Organism

Organism UniProt Comment Textmining
Pinus radiata A0A059SVJ4 or Pinus insignis, gene PrXTH1
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Source Tissue

Source Tissue Comment Organism Textmining
seedling
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 Pinus radiata
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the overall xyloglucan-degrading activity in the presence and absence of XG subunit oligosaccharides, donor substrate is tamarind xyloglucan Pinus radiata ?
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 ?

Subunits

Subunits Comment Organism
More three-dimensional structure analysis of PrXTH1 protein using comparative modeling, the structure shows a beta-sandwich structure with the catalytic site in the middle of the protein structure, composed of residues Glu79, Asp81, and Glu83 Pinus radiata

Synonyms

Synonyms Comment Organism
XTH
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 Pinus radiata
xyloglucan endo-transglycosylase/hydrolase
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 Pinus radiata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pinus radiata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Pinus radiata

Expression

Organism Comment Expression
Pinus radiata gene PrXTH1 upregulation by ethylene up

General Information

General Information Comment Organism
evolution xyloglucan endotransglycosylase/hydrolase (XTH) enzymes belong to the glycosyl hydrolase family 16 (GH16) superfamily, phylogenetic analysis, gene PrXTH1 belongs to group I of XTHs Pinus radiata
additional information PrXTH1 homology structure modeling using crystal structure of poplar xyloglucan endotransglycosylase Ptt-XET16A, PDB ID 1UMZ Pinus radiata
physiological function gene PrXTH1 shows regulation by ethylene, indicating that it might be a target in the hormonal cascade during the inclination response Pinus radiata