Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 14 mM, 30% inhibition | Sphagnum fallax | |
cis-p-coumaric acid | inhibits the rection above 1 mM concentration | Sphagnum fallax | |
NaCl | 0.4 M, 40% inhibition | Sphagnum fallax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
cis-p-coumarate | - |
Sphagnum fallax | |
0.11 | - |
UDP-glucose | cosubstrate | Sphagnum fallax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
gel filtration | Sphagnum fallax |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + cis-coniferyl alcohol + H2O | Fagus grandifolia | - |
? | - |
? | |
UDP-glucose + cis-p-coumarate + H2O | Sphagnum fallax | - |
4'-O-beta-D-glucosyl-cis-p-coumarate + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fagus grandifolia | - |
american beech | - |
Sphagnum fallax | - |
peat moss | - |
Purification (Comment) | Organism |
---|---|
partially, 600fold | Sphagnum fallax |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.054 | - |
- |
Sphagnum fallax |
Storage Stability | Organism |
---|---|
-18°C, freezing results in a loss of activity of more than 50% | Sphagnum fallax |
4°C, loses more than 25% of its activity within 24 h, 5% glycerol causes total loss of activity after 48 h | Sphagnum fallax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-caffeic acid + UDP-glucose + H2O | enzyme activity of 10% compared with that using cis-p-coumaric acid as substrate | Sphagnum fallax | 4'-O-beta-D-glucosyl-cis-caffeate + UDP | - |
? | |
cis-p-coumarate + UDP-glucose + H2O | - |
Sphagnum fallax | 4'-O-beta-D-glucosyl-cis-p-coumarate + UDP | - |
? | |
UDP-glucose + cis-coniferyl alcohol + H2O | - |
Fagus grandifolia | ? | - |
? | |
UDP-glucose + cis-p-coumarate | - |
Sphagnum fallax | 4'-O-beta-D-glucosyl-cis-p-coumarate + UDP | - |
? | |
UDP-glucose + cis-p-coumarate + H2O | - |
Sphagnum fallax | 4'-O-beta-D-glucosyl-cis-p-coumarate + UDP | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.3 | - |
100 mM glycine KOH | Sphagnum fallax |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.3 | - |
Sphagnum fallax |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.8 | 9.5 | below pH 7.8 and above pH 9.5 rapid drop in enzyme activity | Sphagnum fallax |