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Literature summary for 2.4.1.21 extracted from

  • Cuesta-Seijo, J.A.; Nielsen, M.M.; Ruzanski, C.; Krucewicz, K.; Beeren, S.R.; Rydhal, M.G.; Yoshimura, Y.; Striebeck, A.; Motawia, M.S.; Willats, W.G.; Palcic, M.M.
    In vitro biochemical characterization of all barley endosperm starch synthases (2015), Front. Plant Sci., 6, 1265.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 ADP-alpha-D-glucose isoform GBSSI, with maltobiose as cosubstrate, at pH 8.5 and 30°C Hordeum vulgare
0.31
-
 ADP-alpha-D-glucose isoform SSIV, with maltotriose as cosubstrate, at pH 8.5 and 30°C Hordeum vulgare
0.44
-
 ADP-alpha-D-glucose isoform SSIIIb, with maltotriose as cosubstrate, at pH 8.5 and 30°C Hordeum vulgare
0.63
-
 ADP-alpha-D-glucose isoform SSI with maltotriose as cosubstrate, at pH 8.5 and 30°C Hordeum vulgare
46.32
-
 maltopentaose isoform GBSSI, at pH 8.5 and 30°C Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
amyloplast starch grain
-
 Hordeum vulgare 9568
-
chloroplast
-
 Hordeum vulgare 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 x * 59000, isoform GBSSI, calculated from amino acid sequence Hordeum vulgare
67500
-
 x * 67500, isoform SSI, calculated from amino acid sequence Hordeum vulgare
81600
-
 x * 81600, isoform SSIIa, calculated from amino acid sequence Hordeum vulgare
98300
-
 x * 98300, isoform SSIV, calculated from amino acid sequence Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n Hordeum vulgare
-
 ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare F2CUZ5
-
-
Hordeum vulgare G1FUP7
-
-
Hordeum vulgare I1W6L2
-
-
Hordeum vulgare P09842
-
-
Hordeum vulgare Q8H1Y8
-
-
Hordeum vulgare Q9M5A3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
 Hordeum vulgare
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + 3-phosphomaltose
-
 Hordeum vulgare ADP + ?
-
 ?
ADP-alpha-D-glucose + amylopectin
-
 Hordeum vulgare ADP + ?
-
 ?
ADP-alpha-D-glucose + amylopectin high activity Hordeum vulgare ADP + ?
-
 ?
ADP-alpha-D-glucose + glycogen
-
 Hordeum vulgare ADP + ?
-
 ?
ADP-alpha-D-glucose + glycogen high activity Hordeum vulgare ADP + ?
-
 ?
ADP-alpha-D-glucose + maltoheptaose
-
 Hordeum vulgare ADP + maltooctaose
-
 ?
ADP-alpha-D-glucose + maltohexaose
-
 Hordeum vulgare ADP + maltoheptaose
-
 ?
ADP-alpha-D-glucose + maltooctaose
-
 Hordeum vulgare ADP + maltononaose
-
 ?
ADP-alpha-D-glucose + maltopentaose
-
 Hordeum vulgare ADP + maltohexaose
-
 ?
ADP-alpha-D-glucose + maltose
-
 Hordeum vulgare ADP + maltotriose
-
 ?
ADP-alpha-D-glucose + maltotetraose
-
 Hordeum vulgare ADP + maltopentaose
-
 ?
ADP-alpha-D-glucose + maltotriose
-
 Hordeum vulgare ADP + maltotetraose
-
 ?
ADP-alpha-D-glucose + soluble starch
-
 Hordeum vulgare ADP + ?
-
 ?
ADP-alpha-D-glucose + soluble starch high activity Hordeum vulgare ADP + ?
-
 ?
ADP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n
-
 Hordeum vulgare ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
additional information no significant activity with D-glucose as acceptor substrate Hordeum vulgare ?
-
 ?
additional information UDP-alpha-D-glucose is a substrate for isoforms GBSSI, SSI, and SSII but not for SSIII and SSIV Hordeum vulgare ?
-
 ?
additional information UDP-alpha-D-glucose is a substrate for isoforms GBSSI, SSI, and SSIIa but not for SSIII and SSIV Hordeum vulgare ?
-
 ?
additional information UDP-alpha-D-glucose is not a substrate for isoform SSIV Hordeum vulgare ?
-
 ?
additional information UDP-alpha-D-glucose is not a substrate for SSIII Hordeum vulgare ?
-
 ?

Subunits

Subunits Comment Organism
? x * 59000, isoform GBSSI, calculated from amino acid sequence Hordeum vulgare
? x * 67500, isoform SSI, calculated from amino acid sequence Hordeum vulgare
? x * 81600, isoform SSIIa, calculated from amino acid sequence Hordeum vulgare
? x * 98300, isoform SSIV, calculated from amino acid sequence Hordeum vulgare

Synonyms

Synonyms Comment Organism
GBSSI isoform Hordeum vulgare
SSI isoform Hordeum vulgare
SSIIa isoform Hordeum vulgare
SSIIIa isoform Hordeum vulgare
SSIIIb isoform Hordeum vulgare
SSIV isoform Hordeum vulgare
starch synthase
-
 Hordeum vulgare

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
39.8
-
 temperature at which 50% of the activity of isoform GBSSI remains after 15 min incubation Hordeum vulgare
40.1
-
 temperature at which 50% of the activity of isoform SSIIa remains after 15 min incubation Hordeum vulgare
43.8
-
 temperature at which 50% of the activity of isoform SSI remains after 15 min incubation Hordeum vulgare
46.3
-
 temperature at which 50% of the activity of isoform SSIIIb remains after 15 min incubation Hordeum vulgare
47.4
-
 temperature at which 50% of the activity of isoform SSIV remains after 15 min incubation Hordeum vulgare