Molecular cloning and characterization of a novel gene encoding limonoid UDP-glucosyltransferase in Citrus

Kita, M.; Hirata, Y.; Moriguchi, T.; Endo-Inagaki, T.; Matsumoto, R.; Hasegawa, S.; Suhayda, C.G.; Omura, M.; FEBS Lett. 469, 173-178 (2000)

Data extracted from this reference:

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

limonin + UDP-glucose

Citrus sinensis

-

glucosyl-limonin + UDP

-

-

?

limonin + UDP-glucose

Citrus unshiu

-

glucosyl-limonin + UDP

-

-

?

nomilin + UDP-glucose

Citrus sinensis

-

UDP + nomilin 17-beta-D-glucopyranoside

-

-

?

nomilin + UDP-glucose

Citrus unshiu

-

UDP + nomilin 17-beta-D-glucopyranoside

-

-

?

Reaction

Commentary

Organism

Reaction ID

UDP-glucose + limonin = glucosyl-limonin + UDP

the enzyme purified from navel orange albedo tissue also acts on the related tetranortriterpenoid nomilin

Citrus sinensis

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

limonin + UDP-glucose

-

288691

Citrus sinensis

glucosyl-limonin + UDP

-

-

-

?

limonin + UDP-glucose

-

288691

Citrus unshiu

glucosyl-limonin + UDP

-

-

-

?

nomilin + UDP-glucose

-

288691

Citrus sinensis

UDP + nomilin 17-beta-D-glucopyranoside

-

-

-

?

nomilin + UDP-glucose

-

288691

Citrus unshiu

UDP + nomilin 17-beta-D-glucopyranoside

-

-

-

?

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

limonin + UDP-glucose

Citrus sinensis

-

glucosyl-limonin + UDP

-

-

?

limonin + UDP-glucose

Citrus unshiu

-

glucosyl-limonin + UDP

-

-

?

nomilin + UDP-glucose

Citrus sinensis

-

UDP + nomilin 17-beta-D-glucopyranoside

-

-

?

nomilin + UDP-glucose

Citrus unshiu

-

UDP + nomilin 17-beta-D-glucopyranoside

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

limonin + UDP-glucose

-

288691

Citrus sinensis

glucosyl-limonin + UDP

-

-

-

?

limonin + UDP-glucose

-

288691

Citrus unshiu

glucosyl-limonin + UDP

-

-

-

?

nomilin + UDP-glucose

-

288691

Citrus sinensis

UDP + nomilin 17-beta-D-glucopyranoside

-

-

-

?

nomilin + UDP-glucose

-

288691

Citrus unshiu

UDP + nomilin 17-beta-D-glucopyranoside

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

736296

Devaiah

Identification, recombinant ex ...

Citrus x paradisi

J. Agric. Food Chem.

64

1957-1969

2016

-

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1

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-

-

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1

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1

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1

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1

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1

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1

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1

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1

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2

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1

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1

-

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722071

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Possible role of carboxyl and ...

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1

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1

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1

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6

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1

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1

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1

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1

-

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1

1

2

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1

1

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684923

Zaare-Nahandi

Delay expression of limonoid U ...

Citrus aurantium, Citrus x paradisi, Citrus sinensis, Citrus unshiu, Citrus limettioides

Biochem. Biophys. Res. Commun.

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2008

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5

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6

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5

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10

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5

5

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-

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-

-

6

-

-

5

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

658581

Kita

Allelic structures of UDP-gluc ...

Citrus sinensis, Citrus unshiu

Euphytica

132

87-94

2003

-

2

-

-

-

-

-

-

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2

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-

-

-

1

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5

-

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2

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1

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-

658786

Karim

Isolation and characterization ...

Citrus maxima, Citrus maxima Osbeck

Food Chem.

76

431-436

2002

-

-

-

-

-

-

7

2

-

3

-

-

-

2

-

-

1

-

-

1

1

1

2

-

-

1

1

-

-

1

1

2

-

-

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-

-

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-

-

7

-

2

-

3

-

-

-

-

-

1

-

1

1

1

2

-

1

1

-

-

1

1

2

-

-

-

-

-

-

-

660114

Endo

-

Modification of limonoid metab ...

Citrus sinensis

Plant Biotechnol.

19

397-403

2002

-

-

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1

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1

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660341

Karim

-

Preparation and properties of ...

Citrus maxima

Proc. Biochem.

38

809-814

2002

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1

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1

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4

-

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1

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1

2

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1

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1

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4

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1

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1

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2

1

2

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3

-

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-

-

-

288691

Kita

Molecular cloning and characte ...

Citrus sinensis, Citrus unshiu

FEBS Lett.

469

173-178

2000

-

2

1

-

-

-

-

-

1

-

1

4

-

7

-

-

1

1

-

3

-

1

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

1

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-

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-

1

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1

4

-

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1

-

3

-

1

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

288690

Sim

-

Purification of limonoid gluco ...

Citrus aurantium, Citrus maxima, Citrus limonia, Citrus sinensis, Citrus unshiu, Fragaria vesca

Phytochemistry

46

33-37

1997

-

1

-

-

-

-

1

-

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1

2

8

-

7

-

-

1

1

-

1

1

1

9

1

-

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-

-

-

1

1

1

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

1

2

8

-

-

-

1

-

1

1

1

9

1

-

-

-

-

1

1

1

-

-

-

-

-

-

-