BRENDA - Enzyme Database
show all sequences of 2.4.1.210

Isolation and characterization of limonoid glucosyltransferase from pummelo albedo tissue

Karim, M.R.; Hashinaga, F.; Food Chem. 76, 431-436 (2002)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
10 mM, 13% inhibition
Citrus maxima
citraconic anhydride
2 mM, 44% inhibition
Citrus maxima
Cu2+
5 mM, 58% inhibition
Citrus maxima
diethyl dicarbonate
1 mM, 76% inhibition
Citrus maxima
EDTA
1 mM, complete inactivation
Citrus maxima
Hg2+
1 mM, 33% inhibition
Citrus maxima
Mg2+
10 mM, 22% inhibition
Citrus maxima
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
UDP-glucose
pH 7.8, 37C
Citrus maxima
0.2
-
limonin
pH 7.8, 37C
Citrus maxima
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
5 mM, 30% stimulation
Citrus maxima
Mn2+
5 mM, 33% stimulation
Citrus maxima
Zn2+
5 mM, 13% stimulation
Citrus maxima
Organism
Organism
UniProt
Commentary
Textmining
Citrus maxima
-
Osbeck
-
Citrus maxima Osbeck
-
Osbeck
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Citrus maxima
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
albedo tissue
Citrus maxima
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.013
-
-
Citrus maxima
Storage Stability
Storage Stability
Organism
4C, pH 7.5, 10 mM Tris-HCl, enzyme retains 95% of the activity after 3 months, enzyme retains 73% of the activity after 9 months
Citrus maxima
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
UDP-glucose + limonin
-
658786
Citrus maxima
glucosyl-limonin + UDP
-
-
-
?
UDP-glucose + limonin
-
658786
Citrus maxima Osbeck
glucosyl-limonin + UDP
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
-
Citrus maxima
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
20
60
20C: about 40% of maximal activity, 60C: about 40% of maximal activity
Citrus maxima
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Citrus maxima
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
9.5
pH 6.5: about 70% of maximal activity, pH 9.5: about 55% of maximal activity
Citrus maxima
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
3.5
9.5
4C, 12 h, stable
Citrus maxima
10.5
-
4C, 12 h, 62% loss of activity
Citrus maxima
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
10 mM, 13% inhibition
Citrus maxima
citraconic anhydride
2 mM, 44% inhibition
Citrus maxima
Cu2+
5 mM, 58% inhibition
Citrus maxima
diethyl dicarbonate
1 mM, 76% inhibition
Citrus maxima
EDTA
1 mM, complete inactivation
Citrus maxima
Hg2+
1 mM, 33% inhibition
Citrus maxima
Mg2+
10 mM, 22% inhibition
Citrus maxima
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
UDP-glucose
pH 7.8, 37C
Citrus maxima
0.2
-
limonin
pH 7.8, 37C
Citrus maxima
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
5 mM, 30% stimulation
Citrus maxima
Mn2+
5 mM, 33% stimulation
Citrus maxima
Zn2+
5 mM, 13% stimulation
Citrus maxima
Purification (Commentary) (protein specific)
Commentary
Organism
-
Citrus maxima
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
albedo tissue
Citrus maxima
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.013
-
-
Citrus maxima
Storage Stability (protein specific)
Storage Stability
Organism
4C, pH 7.5, 10 mM Tris-HCl, enzyme retains 95% of the activity after 3 months, enzyme retains 73% of the activity after 9 months
Citrus maxima
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
UDP-glucose + limonin
-
658786
Citrus maxima
glucosyl-limonin + UDP
-
-
-
?
UDP-glucose + limonin
-
658786
Citrus maxima Osbeck
glucosyl-limonin + UDP
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
-
Citrus maxima
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
20
60
20C: about 40% of maximal activity, 60C: about 40% of maximal activity
Citrus maxima
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Citrus maxima
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
9.5
pH 6.5: about 70% of maximal activity, pH 9.5: about 55% of maximal activity
Citrus maxima
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
3.5
9.5
4C, 12 h, stable
Citrus maxima
10.5
-
4C, 12 h, 62% loss of activity
Citrus maxima
Other publictions for EC 2.4.1.210
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736296
Devaiah
Identification, recombinant ex ...
Citrus x paradisi
J. Agric. Food Chem.
64
1957-1969
2016
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1
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1
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1
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2
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1
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1
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1
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1
-
1
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1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
722071
Karim
-
Possible role of carboxyl and ...
Citrus maxima x Citrus paradisi
Chin. J. Catal.
31
1445-1451
2010
-
1
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-
-
-
6
1
-
-
-
1
-
1
-
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1
-
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1
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1
1
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1
1
2
-
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1
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6
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1
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1
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1
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1
-
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1
1
2
-
-
-
1
1
-
-
-
684923
Zaare-Nahandi
Delay expression of limonoid U ...
Citrus aurantium, Citrus limettioides, Citrus sinensis, Citrus unshiu, Citrus x paradisi
Biochem. Biophys. Res. Commun.
371
59-62
2008
-
5
5
-
-
-
-
-
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-
-
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11
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-
6
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5
-
10
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5
5
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6
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5
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-
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-
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658581
Kita
-
Allelic structures of UDP-gluc ...
Citrus sinensis, Citrus unshiu
Euphytica
132
87-94
2003
-
2
-
-
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-
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2
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1
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5
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2
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1
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-
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-
-
-
-
-
-
658786
Karim
-
Isolation and characterization ...
Citrus maxima, Citrus maxima Osbeck
Food Chem.
76
431-436
2002
-
-
-
-
-
-
7
2
-
3
-
-
-
2
-
-
1
-
-
1
1
1
2
-
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1
1
-
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1
1
2
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-
-
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7
-
2
-
3
-
-
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-
-
1
-
1
1
1
2
-
1
1
-
-
1
1
2
-
-
-
-
-
-
-
660114
Endo
-
Modification of limonoid metab ...
Citrus sinensis
Plant Biotechnol.
19
397-403
2002
-
-
-
-
-
-
-
-
-
-
-
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1
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1
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
660341
Karim
-
Preparation and properties of ...
Citrus maxima
Proc. Biochem.
38
809-814
2002
-
1
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1
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4
-
-
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1
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1
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1
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1
2
1
2
-
3
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1
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1
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4
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1
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1
-
2
1
2
-
3
-
-
-
-
-
-
-
-
-
288691
Kita
Molecular cloning and characte ...
Citrus sinensis, Citrus unshiu
FEBS Lett.
469
173-178
2000
-
2
1
-
-
-
-
-
1
-
1
4
-
5
-
-
1
1
-
3
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1
4
-
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2
1
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1
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1
4
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1
-
3
-
1
4
-
-
-
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-
-
-
-
-
-
-
-
-
-
288690
Sim
-
Purification of limonoid gluco ...
Citrus aurantium, Citrus limonia, Citrus maxima, Citrus sinensis, Citrus unshiu, Fragaria vesca
Phytochemistry
46
33-37
1997
-
1
-
-
-
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1
-
-
1
2
8
-
6
-
-
1
1
-
1
1
1
9
1
-
-
-
-
-
1
1
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
2
8
-
-
-
1
-
1
1
1
9
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-