BRENDA - Enzyme Database
show all sequences of 2.4.1.210

Purification of limonoid glucosyltransferase from navel orange albedo tissue

Sim, M.K.; Lim, B.C.; Phytochemistry 46, 33-37 (1997)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
nutrition
limonoid glucosides are important compounds not only for the processing industry but also for the consumer, bitterness due to limonoids is an important economic problem in commercial citrus juice production, limonoid aglycones are converted to nonbitter glucosides by the GTase, enhancement of the limonoid GTase activity through genetic engineering could reduce aglycone concentration, insertion of a gene encoding for GTase into commercial cultivars could create transgenic citrus varieties producing fruits potentially free of limonoid bitterness
Citrus sinensis
Inhibitors
Inhibitors
Commentary
Organism
Structure
PO43-
-
Fragaria vesca
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
0.05 mM stimulates enzyme activity by 66% over basal activity
Citrus sinensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
Newhall navel orange, SDS-PAGE
Citrus sinensis
58000
-
Frost navel orange, SDS-PAGE
Citrus sinensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
limonin + UDP-glucose
Citrus sinensis
-
glucosyl-limonin + UDP
-
-
?
limonin + UDP-glucose
Citrus unshiu
-
glucosyl-limonin + UDP
-
-
?
limonin + UDP-glucose
Citrus aurantium
-
glucosyl-limonin + UDP
-
-
?
limonin + UDP-glucose
Fragaria vesca
-
glucosyl-limonin + UDP
-
-
?
limonin + UDP-glucose
Citrus maxima
-
glucosyl-limonin + UDP
-
-
?
limonin + UDP-glucose
Citrus limonia
-
glucosyl-limonin + UDP
-
-
?
nomilin + UDP-glucose
Citrus sinensis
-
UDP + nomilin 17-beta-D-glucopyranoside
-
-
?
nomilin + UDP-glucose
Citrus unshiu
-
UDP + nomilin 17-beta-D-glucopyranoside
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Citrus aurantium
-
pommelo hybrid
-
Citrus limonia
-
citron
-
Citrus maxima
-
grapefruit, pommelo hybrid Oroblanco; pommelo
-
Citrus sinensis
-
Frost navel orange, Newhall navel orange
-
Citrus unshiu
-
mandarin orange
-
Fragaria vesca
-
strawberry
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Citrus sinensis
Reaction
Reaction
Commentary
Organism
Reaction ID
UDP-glucose + limonin = glucosyl-limonin + UDP
the enzyme purified from navel orange albedo tissue also acts on the related tetranortriterpenoid nomilin
Citrus sinensis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
albedo
-
Citrus sinensis
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.018
-
-
Citrus sinensis
Storage Stability
Storage Stability
Organism
-80°C, 50 mM Tris-HCl, pH 8.0, all acivity is lost
Citrus sinensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
limonin + UDP-glucose
-
288690
Citrus sinensis
glucosyl-limonin + UDP
-
-
-
?
limonin + UDP-glucose
-
288690
Citrus unshiu
glucosyl-limonin + UDP
-
-
-
?
limonin + UDP-glucose
-
288690
Citrus aurantium
glucosyl-limonin + UDP
-
-
-
?
limonin + UDP-glucose
-
288690
Fragaria vesca
glucosyl-limonin + UDP
-
-
-
?
limonin + UDP-glucose
-
288690
Citrus maxima
glucosyl-limonin + UDP
-
-
-
?
limonin + UDP-glucose
-
288690
Citrus limonia
glucosyl-limonin + UDP
-
-
-
?
nomilin + UDP-glucose
-
288690
Citrus sinensis
glucosyl-nomilin + UDP
-
-
-
?
nomilin + UDP-glucose
-
288690
Citrus sinensis
UDP + nomilin 17-beta-D-glucopyranoside
-
-
-
?
nomilin + UDP-glucose
-
288690
Citrus unshiu
UDP + nomilin 17-beta-D-glucopyranoside
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
56000-58000, SDS-PAGE
Citrus sinensis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Citrus sinensis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
9
-
Citrus sinensis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
-
activity below pH 6.5 is inhibited by a closed D-ring
Citrus sinensis
Application (protein specific)
Application
Commentary
Organism
nutrition
limonoid glucosides are important compounds not only for the processing industry but also for the consumer, bitterness due to limonoids is an important economic problem in commercial citrus juice production, limonoid aglycones are converted to nonbitter glucosides by the GTase, enhancement of the limonoid GTase activity through genetic engineering could reduce aglycone concentration, insertion of a gene encoding for GTase into commercial cultivars could create transgenic citrus varieties producing fruits potentially free of limonoid bitterness
Citrus sinensis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
PO43-
-
Fragaria vesca
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
0.05 mM stimulates enzyme activity by 66% over basal activity
Citrus sinensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
Newhall navel orange, SDS-PAGE
Citrus sinensis
58000
-
Frost navel orange, SDS-PAGE
Citrus sinensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
limonin + UDP-glucose
Citrus sinensis
-
glucosyl-limonin + UDP
-
-
?
limonin + UDP-glucose
Citrus unshiu
-
glucosyl-limonin + UDP
-
-
?
limonin + UDP-glucose
Citrus aurantium
-
glucosyl-limonin + UDP
-
-
?
limonin + UDP-glucose
Fragaria vesca
-
glucosyl-limonin + UDP
-
-
?
limonin + UDP-glucose
Citrus maxima
-
glucosyl-limonin + UDP
-
-
?
limonin + UDP-glucose
Citrus limonia
-
glucosyl-limonin + UDP
-
-
?
nomilin + UDP-glucose
Citrus sinensis
-
UDP + nomilin 17-beta-D-glucopyranoside
-
-
?
nomilin + UDP-glucose
Citrus unshiu
-
UDP + nomilin 17-beta-D-glucopyranoside
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Citrus sinensis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
albedo
-
Citrus sinensis
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.018
-
-
Citrus sinensis
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, 50 mM Tris-HCl, pH 8.0, all acivity is lost
Citrus sinensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
limonin + UDP-glucose
-
288690
Citrus sinensis
glucosyl-limonin + UDP
-
-
-
?
limonin + UDP-glucose
-
288690
Citrus unshiu
glucosyl-limonin + UDP
-
-
-
?
limonin + UDP-glucose
-
288690
Citrus aurantium
glucosyl-limonin + UDP
-
-
-
?
limonin + UDP-glucose
-
288690
Fragaria vesca
glucosyl-limonin + UDP
-
-
-
?
limonin + UDP-glucose
-
288690
Citrus maxima
glucosyl-limonin + UDP
-
-
-
?
limonin + UDP-glucose
-
288690
Citrus limonia
glucosyl-limonin + UDP
-
-
-
?
nomilin + UDP-glucose
-
288690
Citrus sinensis
glucosyl-nomilin + UDP
-
-
-
?
nomilin + UDP-glucose
-
288690
Citrus sinensis
UDP + nomilin 17-beta-D-glucopyranoside
-
-
-
?
nomilin + UDP-glucose
-
288690
Citrus unshiu
UDP + nomilin 17-beta-D-glucopyranoside
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
56000-58000, SDS-PAGE
Citrus sinensis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Citrus sinensis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
9
-
Citrus sinensis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
-
activity below pH 6.5 is inhibited by a closed D-ring
Citrus sinensis
Other publictions for EC 2.4.1.210
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736296
Devaiah
Identification, recombinant ex ...
Citrus x paradisi
J. Agric. Food Chem.
64
1957-1969
2016
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-
1
-
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
722071
Karim
-
Possible role of carboxyl and ...
Citrus maxima x Citrus paradisi
Chin. J. Catal.
31
1445-1451
2010
-
1
-
-
-
-
6
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
-
1
1
-
-
1
1
2
-
-
-
-
-
-
1
-
-
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
1
1
2
-
-
-
1
1
-
-
-
684923
Zaare-Nahandi
Delay expression of limonoid U ...
Citrus aurantium, Citrus limettioides, Citrus sinensis, Citrus unshiu, Citrus x paradisi
Biochem. Biophys. Res. Commun.
371
59-62
2008
-
5
5
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
6
-
-
5
-
10
-
-
-
-
-
-
-
-
-
-
-
-
5
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658581
Kita
-
Allelic structures of UDP-gluc ...
Citrus sinensis, Citrus unshiu
Euphytica
132
87-94
2003
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658786
Karim
-
Isolation and characterization ...
Citrus maxima, Citrus maxima Osbeck
Food Chem.
76
431-436
2002
-
-
-
-
-
-
7
2
-
3
-
-
-
2
-
-
1
-
-
1
1
1
2
-
-
1
1
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
3
-
-
-
-
-
1
-
1
1
1
2
-
1
1
-
-
1
1
2
-
-
-
-
-
-
-
660114
Endo
-
Modification of limonoid metab ...
Citrus sinensis
Plant Biotechnol.
19
397-403
2002
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660341
Karim
-
Preparation and properties of ...
Citrus maxima
Proc. Biochem.
38
809-814
2002
-
1
-
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
1
2
1
2
-
3
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
1
-
2
1
2
-
3
-
-
-
-
-
-
-
-
-
288691
Kita
Molecular cloning and characte ...
Citrus sinensis, Citrus unshiu
FEBS Lett.
469
173-178
2000
-
2
1
-
-
-
-
-
1
-
1
4
-
5
-
-
1
1
-
3
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
1
4
-
-
-
1
-
3
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288690
Sim
-
Purification of limonoid gluco ...
Citrus aurantium, Citrus limonia, Citrus maxima, Citrus sinensis, Citrus unshiu, Fragaria vesca
Phytochemistry
46
33-37
1997
-
1
-
-
-
-
1
-
-
1
2
8
-
6
-
-
1
1
-
1
1
1
9
1
-
-
-
-
-
1
1
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
2
8
-
-
-
1
-
1
1
1
9
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-