Data extracted from this reference:
Application
nutrition
limonoid glucosides are important compounds not only for the processing industry but also for the consumer, bitterness due to limonoids is an important economic problem in commercial citrus juice production, limonoid aglycones are converted to nonbitter glucosides by the GTase, enhancement of the limonoid GTase activity through genetic engineering could reduce aglycone concentration, insertion of a gene encoding for GTase into commercial cultivars could create transgenic citrus varieties producing fruits potentially free of limonoid bitterness
Citrus sinensis
Inhibitors
Metals/Ions
Mn2+
0.05 mM stimulates enzyme activity by 66% over basal activity
Citrus sinensis
Molecular Weight [Da]
56000
Newhall navel orange, SDS-PAGE
Citrus sinensis
58000
Frost navel orange, SDS-PAGE
Citrus sinensis
Natural Substrates/ Products (Substrates)
limonin + UDP-glucose
Citrus sinensis
glucosyl-limonin + UDP
?
limonin + UDP-glucose
Citrus unshiu
glucosyl-limonin + UDP
?
limonin + UDP-glucose
Citrus aurantium
glucosyl-limonin + UDP
?
limonin + UDP-glucose
Fragaria vesca
glucosyl-limonin + UDP
?
limonin + UDP-glucose
Citrus maxima
glucosyl-limonin + UDP
?
limonin + UDP-glucose
Citrus limonia
glucosyl-limonin + UDP
?
nomilin + UDP-glucose
Citrus sinensis
UDP + nomilin 17-beta-D-glucopyranoside
?
nomilin + UDP-glucose
Citrus unshiu
UDP + nomilin 17-beta-D-glucopyranoside
?
Organism
Citrus aurantium
pommelo hybrid
Citrus maxima
grapefruit, pommelo hybrid Oroblanco
Citrus sinensis
Frost navel orange, Newhall navel orange
Citrus unshiu
mandarin orange
Fragaria vesca
strawberry
Purification (Commentary)
Reaction
UDP-glucose + limonin = glucosyl-limonin + UDP
the enzyme purified from navel orange albedo tissue also acts on the related tetranortriterpenoid nomilin
Citrus sinensis
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
-80°C, 50 mM Tris-HCl, pH 8.0, all acivity is lost
Citrus sinensis
Substrates and Products (Substrate)
limonin + UDP-glucose
288690
Citrus sinensis
glucosyl-limonin + UDP
?
limonin + UDP-glucose
288690
Citrus unshiu
glucosyl-limonin + UDP
?
limonin + UDP-glucose
288690
Citrus aurantium
glucosyl-limonin + UDP
?
limonin + UDP-glucose
288690
Fragaria vesca
glucosyl-limonin + UDP
?
limonin + UDP-glucose
288690
Citrus maxima
glucosyl-limonin + UDP
?
limonin + UDP-glucose
288690
Citrus limonia
glucosyl-limonin + UDP
?
nomilin + UDP-glucose
288690
Citrus sinensis
glucosyl-nomilin + UDP
?
nomilin + UDP-glucose
288690
Citrus sinensis
UDP + nomilin 17-beta-D-glucopyranoside
?
nomilin + UDP-glucose
288690
Citrus unshiu
UDP + nomilin 17-beta-D-glucopyranoside
?
Subunits
monomer
56000-58000, SDS-PAGE
Citrus sinensis
pH Optimum
pH Range
pH Stability
6.5
activity below pH 6.5 is inhibited by a closed D-ring
Citrus sinensis
Application (protein specific)
nutrition
limonoid glucosides are important compounds not only for the processing industry but also for the consumer, bitterness due to limonoids is an important economic problem in commercial citrus juice production, limonoid aglycones are converted to nonbitter glucosides by the GTase, enhancement of the limonoid GTase activity through genetic engineering could reduce aglycone concentration, insertion of a gene encoding for GTase into commercial cultivars could create transgenic citrus varieties producing fruits potentially free of limonoid bitterness
Citrus sinensis
Inhibitors (protein specific)
Metals/Ions (protein specific)
Mn2+
0.05 mM stimulates enzyme activity by 66% over basal activity
Citrus sinensis
Molecular Weight [Da] (protein specific)
56000
Newhall navel orange, SDS-PAGE
Citrus sinensis
58000
Frost navel orange, SDS-PAGE
Citrus sinensis
Natural Substrates/ Products (Substrates) (protein specific)
limonin + UDP-glucose
Citrus sinensis
glucosyl-limonin + UDP
?
limonin + UDP-glucose
Citrus unshiu
glucosyl-limonin + UDP
?
limonin + UDP-glucose
Citrus aurantium
glucosyl-limonin + UDP
?
limonin + UDP-glucose
Fragaria vesca
glucosyl-limonin + UDP
?
limonin + UDP-glucose
Citrus maxima
glucosyl-limonin + UDP
?
limonin + UDP-glucose
Citrus limonia
glucosyl-limonin + UDP
?
nomilin + UDP-glucose
Citrus sinensis
UDP + nomilin 17-beta-D-glucopyranoside
?
nomilin + UDP-glucose
Citrus unshiu
UDP + nomilin 17-beta-D-glucopyranoside
?
Purification (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
-80°C, 50 mM Tris-HCl, pH 8.0, all acivity is lost
Citrus sinensis
Substrates and Products (Substrate) (protein specific)
limonin + UDP-glucose
288690
Citrus sinensis
glucosyl-limonin + UDP
?
limonin + UDP-glucose
288690
Citrus unshiu
glucosyl-limonin + UDP
?
limonin + UDP-glucose
288690
Citrus aurantium
glucosyl-limonin + UDP
?
limonin + UDP-glucose
288690
Fragaria vesca
glucosyl-limonin + UDP
?
limonin + UDP-glucose
288690
Citrus maxima
glucosyl-limonin + UDP
?
limonin + UDP-glucose
288690
Citrus limonia
glucosyl-limonin + UDP
?
nomilin + UDP-glucose
288690
Citrus sinensis
glucosyl-nomilin + UDP
?
nomilin + UDP-glucose
288690
Citrus sinensis
UDP + nomilin 17-beta-D-glucopyranoside
?
nomilin + UDP-glucose
288690
Citrus unshiu
UDP + nomilin 17-beta-D-glucopyranoside
?
Subunits (protein specific)
monomer
56000-58000, SDS-PAGE
Citrus sinensis
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
6.5
activity below pH 6.5 is inhibited by a closed D-ring
Citrus sinensis
Other publictions for EC 2.4.1.210
736296
Devaiah
Identification, recombinant ex ...
Citrus x paradisi
J. Agric. Food Chem.
64
1957-1969
2016
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1
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1
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1
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1
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2
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1
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1
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1
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1
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1
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1
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2
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1
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1
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1
1
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-
722071
Karim
-
Possible role of carboxyl and ...
Citrus maxima x Citrus paradisi
Chin. J. Catal.
31
1445-1451
2010
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1
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6
1
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1
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1
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1
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1
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1
2
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1
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6
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1
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1
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1
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1
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1
1
2
-
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1
1
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-
684923
Zaare-Nahandi
Delay expression of limonoid U ...
Citrus aurantium, Citrus x paradisi, Citrus sinensis, Citrus unshiu, Citrus limettioides
Biochem. Biophys. Res. Commun.
371
59-62
2008
-
5
5
-
-
-
-
-
-
-
-
-
-
13
-
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-
6
-
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5
-
10
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5
5
-
-
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6
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658581
Kita
Allelic structures of UDP-gluc ...
Citrus sinensis, Citrus unshiu
Euphytica
132
87-94
2003
-
2
-
-
-
-
-
-
-
-
-
-
-
2
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1
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5
-
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-
2
-
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-
-
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1
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-
-
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-
-
-
-
-
-
-
658786
Karim
Isolation and characterization ...
Citrus maxima, Citrus maxima Osbeck
Food Chem.
76
431-436
2002
-
-
-
-
-
-
7
2
-
3
-
-
-
2
-
-
1
-
-
1
1
1
2
-
-
1
1
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
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7
-
2
-
3
-
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-
-
-
1
-
1
1
1
2
-
1
1
-
-
1
1
2
-
-
-
-
-
-
-
660114
Endo
-
Modification of limonoid metab ...
Citrus sinensis
Plant Biotechnol.
19
397-403
2002
-
-
-
-
-
-
-
-
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-
1
-
-
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-
1
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1
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-
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-
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-
-
-
-
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-
-
-
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660341
Karim
-
Preparation and properties of ...
Citrus maxima
Proc. Biochem.
38
809-814
2002
-
1
-
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
1
2
1
2
-
3
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
1
-
2
1
2
-
3
-
-
-
-
-
-
-
-
-
288691
Kita
Molecular cloning and characte ...
Citrus sinensis, Citrus unshiu
FEBS Lett.
469
173-178
2000
-
2
1
-
-
-
-
-
1
-
1
4
-
7
-
-
1
1
-
3
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
1
4
-
-
-
1
-
3
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288690
Sim
-
Purification of limonoid gluco ...
Citrus aurantium, Citrus maxima, Citrus limonia, Citrus sinensis, Citrus unshiu, Fragaria vesca
Phytochemistry
46
33-37
1997
-
1
-
-
-
-
1
-
-
1
2
8
-
7
-
-
1
1
-
1
1
1
9
1
-
-
-
-
-
1
1
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
2
8
-
-
-
1
-
1
1
1
9
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-