BRENDA - Reference to 2.4.1.210; Id = 288690

nutrition

limonoid glucosides are important compounds not only for the processing industry but also for the consumer, bitterness due to limonoids is an important economic problem in commercial citrus juice production, limonoid aglycones are converted to nonbitter glucosides by the GTase, enhancement of the limonoid GTase activity through genetic engineering could reduce aglycone concentration, insertion of a gene encoding for GTase into commercial cultivars could create transgenic citrus varieties producing fruits potentially free of limonoid bitterness

Citrus sinensis

PO43-

-

Fragaria vesca

Mn2+

0.05 mM stimulates enzyme activity by 66% over basal activity

Citrus sinensis

56000

-

Newhall navel orange, SDS-PAGE

Citrus sinensis

58000

-

Frost navel orange, SDS-PAGE

Citrus sinensis

limonin + UDP-glucose

Citrus sinensis

-

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

Citrus unshiu

-

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

Citrus aurantium

-

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

Fragaria vesca

-

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

Citrus maxima

-

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

Citrus limonia

-

glucosyl-limonin + UDP

-

?

nomilin + UDP-glucose

Citrus sinensis

-

UDP + nomilin 17-beta-D-glucopyranoside

-

?

nomilin + UDP-glucose

Citrus unshiu

-

UDP + nomilin 17-beta-D-glucopyranoside

-

?

Citrus aurantium

-

pommelo hybrid

-

Citrus limonia

-

citron

-

Citrus maxima

-

pommelo

-

Citrus maxima

-

grapefruit, pommelo hybrid Oroblanco

-

Citrus sinensis

-

Frost navel orange, Newhall navel orange

-

Citrus unshiu

-

mandarin orange

-

Fragaria vesca

-

strawberry

-

Citrus sinensis

UDP-glucose + limonin = glucosyl-limonin + UDP

the enzyme purified from navel orange albedo tissue also acts on the related tetranortriterpenoid nomilin

Citrus sinensis

albedo

-

Citrus sinensis

-

0.018

-

Citrus sinensis

-80°C, 50 mM Tris-HCl, pH 8.0, all acivity is lost

Citrus sinensis

limonin + UDP-glucose

-

288690

Citrus sinensis

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

-

288690

Citrus unshiu

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

-

288690

Citrus aurantium

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

-

288690

Fragaria vesca

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

-

288690

Citrus maxima

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

-

288690

Citrus limonia

glucosyl-limonin + UDP

-

?

nomilin + UDP-glucose

-

288690

Citrus sinensis

glucosyl-nomilin + UDP

-

?

nomilin + UDP-glucose

-

288690

Citrus sinensis

UDP + nomilin 17-beta-D-glucopyranoside

-

?

nomilin + UDP-glucose

-

288690

Citrus unshiu

UDP + nomilin 17-beta-D-glucopyranoside

-

?

monomer

56000-58000, SDS-PAGE

Citrus sinensis

8

-

Citrus sinensis

6.5

9

-

Citrus sinensis

6.5

-

activity below pH 6.5 is inhibited by a closed D-ring

Citrus sinensis

nutrition

limonoid glucosides are important compounds not only for the processing industry but also for the consumer, bitterness due to limonoids is an important economic problem in commercial citrus juice production, limonoid aglycones are converted to nonbitter glucosides by the GTase, enhancement of the limonoid GTase activity through genetic engineering could reduce aglycone concentration, insertion of a gene encoding for GTase into commercial cultivars could create transgenic citrus varieties producing fruits potentially free of limonoid bitterness

Citrus sinensis

PO43-

-

Fragaria vesca

Mn2+

0.05 mM stimulates enzyme activity by 66% over basal activity

Citrus sinensis

56000

-

Newhall navel orange, SDS-PAGE

Citrus sinensis

58000

-

Frost navel orange, SDS-PAGE

Citrus sinensis

limonin + UDP-glucose

Citrus sinensis

-

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

Citrus unshiu

-

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

Citrus aurantium

-

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

Fragaria vesca

-

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

Citrus maxima

-

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

Citrus limonia

-

glucosyl-limonin + UDP

-

?

nomilin + UDP-glucose

Citrus sinensis

-

UDP + nomilin 17-beta-D-glucopyranoside

-

?

nomilin + UDP-glucose

Citrus unshiu

-

UDP + nomilin 17-beta-D-glucopyranoside

-

?

-

Citrus sinensis

albedo

-

Citrus sinensis

-

0.018

-

Citrus sinensis

-80°C, 50 mM Tris-HCl, pH 8.0, all acivity is lost

Citrus sinensis

limonin + UDP-glucose

-

288690

Citrus sinensis

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

-

288690

Citrus unshiu

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

-

288690

Citrus aurantium

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

-

288690

Fragaria vesca

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

-

288690

Citrus maxima

glucosyl-limonin + UDP

-

?

limonin + UDP-glucose

-

288690

Citrus limonia

glucosyl-limonin + UDP

-

?

nomilin + UDP-glucose

-

288690

Citrus sinensis

glucosyl-nomilin + UDP

-

?

nomilin + UDP-glucose

-

288690

Citrus sinensis

UDP + nomilin 17-beta-D-glucopyranoside

-

?

nomilin + UDP-glucose

-

288690

Citrus unshiu

UDP + nomilin 17-beta-D-glucopyranoside

-

?

monomer

56000-58000, SDS-PAGE

Citrus sinensis

8

-

Citrus sinensis

6.5

9

-

Citrus sinensis

6.5

-

activity below pH 6.5 is inhibited by a closed D-ring

Citrus sinensis

736296

Devaiah

Identification, recombinant ex ...

Citrus x paradisi

J. Agric. Food Chem.

64

1957-1969

2016

-

1

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

722071

Karim

-

Possible role of carboxyl and ...

Citrus maxima x Citrus paradisi

Chin. J. Catal.

31

1445-1451

2010

-

1

-

6

1

-

1

-

1

-

1

-

1

-

1

-

1

2

-

1

-

6

-

1

-

1

-

1

-

1

-

1

2

-

1

-

684923

Zaare-Nahandi

Delay expression of limonoid U ...

Citrus aurantium, Citrus x paradisi, Citrus sinensis, Citrus unshiu, Citrus limettioides

Biochem. Biophys. Res. Commun.

371

59-62

2008

-

5

-

13

-

6

-

5

-

10

-

5

-

6

-

5

-

658581

Kita

Allelic structures of UDP-gluc ...

Citrus sinensis, Citrus unshiu

Euphytica

132

87-94

2003

-

2

-

2

-

1

-

5

-

2

-

1

-

658786

Karim

Isolation and characterization ...

Citrus maxima, Citrus maxima Osbeck

Food Chem.

76

431-436

2002

-

7

2

-

3

-

2

-

1

-

1

2

-

1

-

1

2

-

7

-

2

-

3

-

1

-

1

2

-

1

-

1

2

-

660114

Endo

-

Modification of limonoid metab ...

Citrus sinensis

Plant Biotechnol.

19

397-403

2002

-

1

-

1

-

1

-

1

-

1

-

660341

Karim

-

Preparation and properties of ...

Citrus maxima

Proc. Biochem.

38

809-814

2002

-

1

-

1

-

4

-

1

-

1

-

1

-

1

2

1

2

-

3

-

1

-

1

-

4

-

1

-

1

-

2

1

2

-

3

-

288691

Kita

Molecular cloning and characte ...

Citrus sinensis, Citrus unshiu

FEBS Lett.

469

173-178

2000

-

2

1

-

1

-

1

4

-

7

-

1

-

3

-

1

4

-

2

1

-

1

-

1

4

-

1

-

3

-

1

4

-

288690

Sim

-

Purification of limonoid gluco ...

Citrus aurantium, Citrus maxima, Citrus limonia, Citrus sinensis, Citrus unshiu, Fragaria vesca

Phytochemistry

46

33-37

1997

-

1

-

1

-

1

2

8

-

7

-

1

-

1

9

1

-

1

-

1

-

1

-

1

2

8

-

1

-

1

9

1

-

1

-

Purification of limonoid glucosyltransferase from navel orange albedo tissue

Data extracted from this reference: