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show all sequences of 2.4.1.210

Molecular cloning and characterization of a novel gene encoding limonoid UDP-glucosyltransferase in Citrus

Kita, M.; Hirata, Y.; Moriguchi, T.; Endo-Inagaki, T.; Matsumoto, R.; Hasegawa, S.; Suhayda, C.G.; Omura, M.; FEBS Lett. 469, 173-178 (2000)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
pharmacological application of limonoid GTase gene
Citrus sinensis
nutrition
-
Citrus sinensis
Cloned(Commentary)
Commentary
Organism
cDNA clone encoding limonoid UDP-glucosyltransferase isolated, single copy gene in the citrus genome
Citrus unshiu
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Citrus sinensis
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57500
-
predicted from amino acid sequence
Citrus unshiu
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
limonin + UDP-glucose
Citrus sinensis
-
glucosyl-limonin + UDP
-
-
?
limonin + UDP-glucose
Citrus unshiu
-
glucosyl-limonin + UDP
-
-
?
nomilin + UDP-glucose
Citrus sinensis
-
UDP + nomilin 17-beta-D-glucopyranoside
-
-
?
nomilin + UDP-glucose
Citrus unshiu
-
UDP + nomilin 17-beta-D-glucopyranoside
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Citrus sinensis
-
sweet navel orange Osb. cv. Washington
-
Citrus unshiu
-
satsuma mandarin Marc. cv. Miyagawa-wase
-
Purification (Commentary)
Commentary
Organism
-
Citrus sinensis
Reaction
Reaction
Commentary
Organism
UDP-glucose + limonin = glucosyl-limonin + UDP
the enzyme purified from navel orange albedo tissue also acts on the related tetranortriterpenoid nomilin
Citrus sinensis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
albedo
inner spongy part of rind
Citrus sinensis
-
Storage Stability
Storage Stability
Organism
-80°C, 10 mM Mes-KOH, pH 7 allows frozen storage with full recovery of activity
Citrus sinensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
limonin + UDP-glucose
-
288691
Citrus sinensis
glucosyl-limonin + UDP
-
-
-
?
limonin + UDP-glucose
-
288691
Citrus unshiu
glucosyl-limonin + UDP
-
-
-
?
nomilin + UDP-glucose
-
288691
Citrus sinensis
UDP + nomilin 17-beta-D-glucopyranoside
-
-
-
?
nomilin + UDP-glucose
-
288691
Citrus unshiu
UDP + nomilin 17-beta-D-glucopyranoside
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
medicine
pharmacological application of limonoid GTase gene
Citrus sinensis
nutrition
-
Citrus sinensis
Cloned(Commentary) (protein specific)
Commentary
Organism
cDNA clone encoding limonoid UDP-glucosyltransferase isolated, single copy gene in the citrus genome
Citrus unshiu
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Citrus sinensis
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57500
-
predicted from amino acid sequence
Citrus unshiu
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
limonin + UDP-glucose
Citrus sinensis
-
glucosyl-limonin + UDP
-
-
?
limonin + UDP-glucose
Citrus unshiu
-
glucosyl-limonin + UDP
-
-
?
nomilin + UDP-glucose
Citrus sinensis
-
UDP + nomilin 17-beta-D-glucopyranoside
-
-
?
nomilin + UDP-glucose
Citrus unshiu
-
UDP + nomilin 17-beta-D-glucopyranoside
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Citrus sinensis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
albedo
inner spongy part of rind
Citrus sinensis
-
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, 10 mM Mes-KOH, pH 7 allows frozen storage with full recovery of activity
Citrus sinensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
limonin + UDP-glucose
-
288691
Citrus sinensis
glucosyl-limonin + UDP
-
-
-
?
limonin + UDP-glucose
-
288691
Citrus unshiu
glucosyl-limonin + UDP
-
-
-
?
nomilin + UDP-glucose
-
288691
Citrus sinensis
UDP + nomilin 17-beta-D-glucopyranoside
-
-
-
?
nomilin + UDP-glucose
-
288691
Citrus unshiu
UDP + nomilin 17-beta-D-glucopyranoside
-
-
-
?
Other publictions for EC 2.4.1.210
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736296
Devaiah
Identification, recombinant ex ...
Citrus x paradisi
J. Agric. Food Chem.
64
1957-1969
2016
-
-
1
-
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
722071
Karim
-
Possible role of carboxyl and ...
Citrus maxima x Citrus paradisi
Chin. J. Catal.
31
1445-1451
2010
-
1
-
-
-
-
6
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
-
1
-
-
1
1
2
-
-
-
-
-
-
1
-
-
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
1
1
2
-
-
-
1
1
-
-
-
684923
Zaare-Nahandi
Delay expression of limonoid U ...
Citrus aurantium, Citrus limettioides, Citrus sinensis, Citrus unshiu, Citrus x paradisi
Biochem. Biophys. Res. Commun.
371
59-62
2008
-
5
5
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
6
-
-
5
-
-
-
-
-
-
-
-
-
-
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-
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5
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658581
Kita
Allelic structures of UDP-gluc ...
Citrus sinensis, Citrus unshiu
Euphytica
132
87-94
2003
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
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-
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-
-
-
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-
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-
-
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-
2
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-
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-
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-
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1
-
-
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-
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-
-
-
-
-
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-
-
-
-
-
-
658786
Karim
Isolation and characterization ...
Citrus maxima, Citrus maxima Osbeck
Food Chem.
76
431-436
2002
-
-
-
-
-
-
7
2
-
3
-
-
-
2
-
-
1
-
-
1
1
1
2
-
1
1
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
3
-
-
-
-
-
1
-
1
1
1
2
-
1
1
-
-
1
1
2
-
-
-
-
-
-
-
660114
Endo
-
Modification of limonoid metab ...
Citrus sinensis
Plant Biotechnol.
19
397-403
2002
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
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-
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
660341
Karim
-
Preparation and properties of ...
Citrus maxima
Proc. Biochem.
38
809-814
2002
-
1
-
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
2
1
2
-
3
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
1
-
2
1
2
-
3
-
-
-
-
-
-
-
-
-
288691
Kita
Molecular cloning and characte ...
Citrus sinensis, Citrus unshiu
FEBS Lett.
469
173-178
2000
-
2
1
-
-
-
-
-
1
-
1
4
-
5
-
-
1
1
-
3
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
1
4
-
-
-
1
-
3
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288690
Sim
-
Purification of limonoid gluco ...
Citrus aurantium, Citrus limonia, Citrus maxima, Citrus sinensis, Citrus unshiu, Fragaria vesca
Phytochemistry
46
33-37
1997
-
1
-
-
-
-
1
-
-
1
2
8
-
6
-
-
1
1
-
1
1
1
9
1
-
-
-
-
1
1
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
2
8
-
-
-
1
-
1
1
1
9
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-