BRENDA - Enzyme Database
show all sequences of 2.4.1.210

Preparation and properties of immobilized pummelo limonoid glucosyltransferase

Karim, M.R.; Hashinaga, F.; Proc. Biochem. 38, 809-814 (2002)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
nutrition
exploration of the possibility of using the enzyme in a bioreactor for debittering citrus juice
Citrus maxima
General Stability
General Stability
Organism
immobilized LGTase retains more than 80% activity up to 15 cycles on cellulose carbonate, nine cycles on chitosan and six cycles on DEAE-Toyopearl
Citrus maxima
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
limonin
pH 7.8, 37°C, soluble enzyme
Citrus maxima
0.0813
-
limonin
pH 7.8, 37°C, enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde
Citrus maxima
0.091
-
limonin
pH 7.8, 37°C, enzyme immobilized covalently on cellulose carbonate
Citrus maxima
0.1266
-
limonin
pH 7.8, 37°C, enzyme immobilized ionically on DEAE-Toyopearl
Citrus maxima
Organism
Organism
UniProt
Commentary
Textmining
Citrus maxima
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Citrus maxima
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
UDP-glucose + limonin
-
660341
Citrus maxima
glucosyl-limonin + UDP
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
LGTase
-
Citrus maxima
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
soluble enzyme and enzyme immobilized ionically on DEAE-Toyopearl (DE-Tp-LGTase)
Citrus maxima
40
-
enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde (Chito-GA-LGTase) and enzyme immobilized covalently on cellulose carbonate (Ce-Ca-LGTase)
Citrus maxima
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
70
20°C: about 55% of maximal activity, 70°C: about 30% of maximal activity
Citrus maxima
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
pH 7.5, 3 h, Ce-Ca-LGTase retains 65.3%, free LGTase retains 19.2%
Citrus maxima
60
-
pH 7.5, 30 min, free enzyme retains 55% of the activity, enzyme immobilized ionically on DEAE-Toyopearl retains 80% of the activity, enzyme immobilized covalently on cellulose carbonate retains 45% of the activity, enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde retains 40% of the activity
Citrus maxima
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde (Chito-GA-LGTase)
Citrus maxima
7.8
-
soluble enzyme or enzyme immobilized covalently on cellulose carbonate (Ce-Ca-LGTase)
Citrus maxima
8.2
-
enzyme immobilized ionically on DEAE-Toyopearl (DE-Tp-LGTase)
Citrus maxima
Application (protein specific)
Application
Commentary
Organism
nutrition
exploration of the possibility of using the enzyme in a bioreactor for debittering citrus juice
Citrus maxima
General Stability (protein specific)
General Stability
Organism
immobilized LGTase retains more than 80% activity up to 15 cycles on cellulose carbonate, nine cycles on chitosan and six cycles on DEAE-Toyopearl
Citrus maxima
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
limonin
pH 7.8, 37°C, soluble enzyme
Citrus maxima
0.0813
-
limonin
pH 7.8, 37°C, enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde
Citrus maxima
0.091
-
limonin
pH 7.8, 37°C, enzyme immobilized covalently on cellulose carbonate
Citrus maxima
0.1266
-
limonin
pH 7.8, 37°C, enzyme immobilized ionically on DEAE-Toyopearl
Citrus maxima
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Citrus maxima
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
UDP-glucose + limonin
-
660341
Citrus maxima
glucosyl-limonin + UDP
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
soluble enzyme and enzyme immobilized ionically on DEAE-Toyopearl (DE-Tp-LGTase)
Citrus maxima
40
-
enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde (Chito-GA-LGTase) and enzyme immobilized covalently on cellulose carbonate (Ce-Ca-LGTase)
Citrus maxima
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
70
20°C: about 55% of maximal activity, 70°C: about 30% of maximal activity
Citrus maxima
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
pH 7.5, 3 h, Ce-Ca-LGTase retains 65.3%, free LGTase retains 19.2%
Citrus maxima
60
-
pH 7.5, 30 min, free enzyme retains 55% of the activity, enzyme immobilized ionically on DEAE-Toyopearl retains 80% of the activity, enzyme immobilized covalently on cellulose carbonate retains 45% of the activity, enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde retains 40% of the activity
Citrus maxima
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde (Chito-GA-LGTase)
Citrus maxima
7.8
-
soluble enzyme or enzyme immobilized covalently on cellulose carbonate (Ce-Ca-LGTase)
Citrus maxima
8.2
-
enzyme immobilized ionically on DEAE-Toyopearl (DE-Tp-LGTase)
Citrus maxima
Other publictions for EC 2.4.1.210
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736296
Devaiah
Identification, recombinant ex ...
Citrus x paradisi
J. Agric. Food Chem.
64
1957-1969
2016
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1
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1
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1
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1
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2
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1
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1
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1
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1
-
1
-
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1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
722071
Karim
-
Possible role of carboxyl and ...
Citrus maxima x Citrus paradisi
Chin. J. Catal.
31
1445-1451
2010
-
1
-
-
-
-
6
1
-
-
-
1
-
1
-
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1
-
-
-
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1
-
1
1
-
-
1
1
2
-
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1
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6
-
1
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1
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1
-
1
-
-
1
1
2
-
-
-
1
1
-
-
-
684923
Zaare-Nahandi
Delay expression of limonoid U ...
Citrus aurantium, Citrus limettioides, Citrus sinensis, Citrus unshiu, Citrus x paradisi
Biochem. Biophys. Res. Commun.
371
59-62
2008
-
5
5
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
6
-
-
5
-
10
-
-
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-
-
-
-
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-
-
5
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658581
Kita
-
Allelic structures of UDP-gluc ...
Citrus sinensis, Citrus unshiu
Euphytica
132
87-94
2003
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
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1
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5
-
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-
2
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1
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-
-
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-
-
658786
Karim
-
Isolation and characterization ...
Citrus maxima, Citrus maxima Osbeck
Food Chem.
76
431-436
2002
-
-
-
-
-
-
7
2
-
3
-
-
-
2
-
-
1
-
-
1
1
1
2
-
-
1
1
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
3
-
-
-
-
-
1
-
1
1
1
2
-
1
1
-
-
1
1
2
-
-
-
-
-
-
-
660114
Endo
-
Modification of limonoid metab ...
Citrus sinensis
Plant Biotechnol.
19
397-403
2002
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
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1
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660341
Karim
-
Preparation and properties of ...
Citrus maxima
Proc. Biochem.
38
809-814
2002
-
1
-
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
1
2
1
2
-
3
-
-
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-
-
1
-
-
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
1
-
2
1
2
-
3
-
-
-
-
-
-
-
-
-
288691
Kita
Molecular cloning and characte ...
Citrus sinensis, Citrus unshiu
FEBS Lett.
469
173-178
2000
-
2
1
-
-
-
-
-
1
-
1
4
-
5
-
-
1
1
-
3
-
1
4
-
-
-
-
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-
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2
1
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1
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1
4
-
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1
-
3
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288690
Sim
-
Purification of limonoid gluco ...
Citrus aurantium, Citrus limonia, Citrus maxima, Citrus sinensis, Citrus unshiu, Fragaria vesca
Phytochemistry
46
33-37
1997
-
1
-
-
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1
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1
2
8
-
6
-
-
1
1
-
1
1
1
9
1
-
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-
-
-
1
1
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
2
8
-
-
-
1
-
1
1
1
9
1
-
-
-
-
1
1
1
-
-
-
-
-
-
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