Application | Comment | Organism |
---|---|---|
nutrition | exploration of the possibility of using the enzyme in a bioreactor for debittering citrus juice | Citrus maxima |
General Stability | Organism |
---|---|
immobilized LGTase retains more than 80% activity up to 15 cycles on cellulose carbonate, nine cycles on chitosan and six cycles on DEAE-Toyopearl | Citrus maxima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
limonin | pH 7.8, 37°C, soluble enzyme | Citrus maxima | |
0.0813 | - |
limonin | pH 7.8, 37°C, enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde | Citrus maxima | |
0.091 | - |
limonin | pH 7.8, 37°C, enzyme immobilized covalently on cellulose carbonate | Citrus maxima | |
0.1266 | - |
limonin | pH 7.8, 37°C, enzyme immobilized ionically on DEAE-Toyopearl | Citrus maxima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus maxima | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Citrus maxima | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + limonin | - |
Citrus maxima | glucosyl-limonin + UDP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LGTase | - |
Citrus maxima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
soluble enzyme and enzyme immobilized ionically on DEAE-Toyopearl (DE-Tp-LGTase) | Citrus maxima |
40 | - |
enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde (Chito-GA-LGTase) and enzyme immobilized covalently on cellulose carbonate (Ce-Ca-LGTase) | Citrus maxima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | 20°C: about 55% of maximal activity, 70°C: about 30% of maximal activity | Citrus maxima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 7.5, 3 h, Ce-Ca-LGTase retains 65.3%, free LGTase retains 19.2% | Citrus maxima |
60 | - |
pH 7.5, 30 min, free enzyme retains 55% of the activity, enzyme immobilized ionically on DEAE-Toyopearl retains 80% of the activity, enzyme immobilized covalently on cellulose carbonate retains 45% of the activity, enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde retains 40% of the activity | Citrus maxima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde (Chito-GA-LGTase) | Citrus maxima |
7.8 | - |
soluble enzyme or enzyme immobilized covalently on cellulose carbonate (Ce-Ca-LGTase) | Citrus maxima |
8.2 | - |
enzyme immobilized ionically on DEAE-Toyopearl (DE-Tp-LGTase) | Citrus maxima |