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show all sequences of 2.4.1.210

Possible role of carboxyl and imidazole groups in the catalysis of pummelo limonoid glucosyltransferase

Karim, M.; Hashinaga, F.; Chin. J. Catal. 31, 1445-1451 (2010)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
food industry
Limonoid glucosyltransferase is an enzyme that catalyzes the conversion of bitter limonoid into non-bitter limonoid glucoside while retaining the health benefit of limonoids in the juice. The immobilization of this enzyme in a column can solve the juice bitterness problem
Citrus maxima x Citrus paradisi
Inhibitors
Inhibitors
Commentary
Organism
Structure
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
modifies tryptophan residues
Citrus maxima x Citrus paradisi
3-nitro-L-tyrosine ethylester
modifies tryptophan residues
Citrus maxima x Citrus paradisi
citraconic anhydride
modifies lysine residues
Citrus maxima x Citrus paradisi
diethyldicarbonate
modifies histidine residues and inactivates the enzyme, pseudo first order kinetics. When the histidine modification is reversed by hydroxylamine treatment, 79% of the activity is restored
Citrus maxima x Citrus paradisi
additional information
inactivation of the enzyme following modification of carboxyl and imidazole moieties is a consequence of a loss in substrate binding and catalysis in the glucosyltransfer reaction. No inhibition by serine modifiying diisopropyl fluorophosphate and cysteine modifying 4-chloromercuribenzoate and iodoacetamide
Citrus maxima x Citrus paradisi
N-bromosuccinimide
modifies tryptophan residues and inactivates the enzyme, loss of LGTase activity by NBS treatment is partially protected by pre-incubating the enzyme with excess limonin
Citrus maxima x Citrus paradisi
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
limonin
pH 7.0-8.4, 25C
Citrus maxima x Citrus paradisi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-glucose + limonin
Citrus maxima x Citrus paradisi
-
glucosyl-limonin + UDP
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Citrus maxima x Citrus paradisi
-
-
-
Reaction
Reaction
Commentary
Organism
UDP-glucose + limonin = glucosyl-limonin + UDP
catalytic reaction mechanism, overview
Citrus maxima x Citrus paradisi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + limonin
-
722071
Citrus maxima x Citrus paradisi
glucosyl-limonin + UDP
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Citrus maxima x Citrus paradisi
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
91.7
-
limonin
pH 7.0-8.4, 25C
Citrus maxima x Citrus paradisi
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Citrus maxima x Citrus paradisi
pH Range
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
at an acidic pH the D-ring of the substrate that should be in an open form is closed and glycosylation does not occur. The decrease in activity at acidic pH also indicates that an essential residue participates in catalytic functioning while in the unprotonated state
Citrus maxima x Citrus paradisi
5.5
9.5
activity range
Citrus maxima x Citrus paradisi
Application (protein specific)
Application
Commentary
Organism
food industry
Limonoid glucosyltransferase is an enzyme that catalyzes the conversion of bitter limonoid into non-bitter limonoid glucoside while retaining the health benefit of limonoids in the juice. The immobilization of this enzyme in a column can solve the juice bitterness problem
Citrus maxima x Citrus paradisi
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
modifies tryptophan residues
Citrus maxima x Citrus paradisi
3-nitro-L-tyrosine ethylester
modifies tryptophan residues
Citrus maxima x Citrus paradisi
citraconic anhydride
modifies lysine residues
Citrus maxima x Citrus paradisi
diethyldicarbonate
modifies histidine residues and inactivates the enzyme, pseudo first order kinetics. When the histidine modification is reversed by hydroxylamine treatment, 79% of the activity is restored
Citrus maxima x Citrus paradisi
additional information
inactivation of the enzyme following modification of carboxyl and imidazole moieties is a consequence of a loss in substrate binding and catalysis in the glucosyltransfer reaction. No inhibition by serine modifiying diisopropyl fluorophosphate and cysteine modifying 4-chloromercuribenzoate and iodoacetamide
Citrus maxima x Citrus paradisi
N-bromosuccinimide
modifies tryptophan residues and inactivates the enzyme, loss of LGTase activity by NBS treatment is partially protected by pre-incubating the enzyme with excess limonin
Citrus maxima x Citrus paradisi
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
limonin
pH 7.0-8.4, 25C
Citrus maxima x Citrus paradisi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-glucose + limonin
Citrus maxima x Citrus paradisi
-
glucosyl-limonin + UDP
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + limonin
-
722071
Citrus maxima x Citrus paradisi
glucosyl-limonin + UDP
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Citrus maxima x Citrus paradisi
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
91.7
-
limonin
pH 7.0-8.4, 25C
Citrus maxima x Citrus paradisi
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Citrus maxima x Citrus paradisi
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
at an acidic pH the D-ring of the substrate that should be in an open form is closed and glycosylation does not occur. The decrease in activity at acidic pH also indicates that an essential residue participates in catalytic functioning while in the unprotonated state
Citrus maxima x Citrus paradisi
5.5
9.5
activity range
Citrus maxima x Citrus paradisi
General Information
General Information
Commentary
Organism
additional information
limonoid glucosyltransferase is an enzyme that catalyzes the conversion of bitter limonoid into non-bitter limonoid glucoside while retaining the health benefit of limonoids in the juice
Citrus maxima x Citrus paradisi
General Information (protein specific)
General Information
Commentary
Organism
additional information
limonoid glucosyltransferase is an enzyme that catalyzes the conversion of bitter limonoid into non-bitter limonoid glucoside while retaining the health benefit of limonoids in the juice
Citrus maxima x Citrus paradisi
Other publictions for EC 2.4.1.210
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736296
Devaiah
Identification, recombinant ex ...
Citrus x paradisi
J. Agric. Food Chem.
64
1957-1969
2016
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2
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1
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1
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1
1
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722071
Karim
-
Possible role of carboxyl and ...
Citrus maxima x Citrus paradisi
Chin. J. Catal.
31
1445-1451
2010
-
1
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6
1
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6
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1
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1
2
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1
1
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684923
Zaare-Nahandi
Delay expression of limonoid U ...
Citrus aurantium, Citrus limettioides, Citrus sinensis, Citrus unshiu, Citrus x paradisi
Biochem. Biophys. Res. Commun.
371
59-62
2008
-
5
5
-
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-
-
-
-
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-
11
-
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6
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5
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5
5
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6
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5
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-
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658581
Kita
Allelic structures of UDP-gluc ...
Citrus sinensis, Citrus unshiu
Euphytica
132
87-94
2003
-
2
-
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-
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-
-
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2
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1
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2
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1
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658786
Karim
Isolation and characterization ...
Citrus maxima, Citrus maxima Osbeck
Food Chem.
76
431-436
2002
-
-
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7
2
-
3
-
-
-
2
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1
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1
1
1
2
-
1
1
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1
1
2
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7
-
2
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3
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1
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1
1
1
2
-
1
1
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1
1
2
-
-
-
-
-
-
-
660114
Endo
-
Modification of limonoid metab ...
Citrus sinensis
Plant Biotechnol.
19
397-403
2002
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1
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660341
Karim
-
Preparation and properties of ...
Citrus maxima
Proc. Biochem.
38
809-814
2002
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1
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1
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4
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1
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1
2
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3
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1
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4
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1
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1
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2
1
2
-
3
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288691
Kita
Molecular cloning and characte ...
Citrus sinensis, Citrus unshiu
FEBS Lett.
469
173-178
2000
-
2
1
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-
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1
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1
4
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5
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1
1
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3
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4
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1
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4
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1
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3
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1
4
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288690
Sim
-
Purification of limonoid gluco ...
Citrus aurantium, Citrus limonia, Citrus maxima, Citrus sinensis, Citrus unshiu, Fragaria vesca
Phytochemistry
46
33-37
1997
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1
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1
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1
2
8
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6
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1
1
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1
1
1
9
1
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1
1
1
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1
2
8
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1
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1
1
1
9
1
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1
1
1
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