BRENDA - Enzyme Database show
show all sequences of 2.4.1.210

Identification, recombinant expression, and biochemical analysis of putative secondary product glucosyltransferases from Citrus paradisi

Devaiah, S.P.; Owens, D.K.; Sibhatu, M.B.; Sarkar, T.R.; Strong, C.L.; Mallampalli, V.K.; Asiago, J.; Cooke, J.; Kiser, S.; Lin, Z.; Wamucho, A.; Hayford, D.; Williams, B.E.; Loftis, P.; Berhow, M.; Pike, L.M.; McIntosh, C.A.; J. Agric. Food Chem. 64, 1957-1969 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene SLLGT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli and in Pichia pastoris
Citrus x paradisi
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
required
Citrus x paradisi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-glucose + limonin
Citrus x paradisi
-
glucosyl-limonin + UDP
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Citrus x paradisi
B2YGX8
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme from Escherichia coli and from Pichia pastoris
Citrus x paradisi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + limonin
-
736296
Citrus x paradisi
glucosyl-limonin + UDP
-
-
-
?
UDP-glucose + limonoate A-ring-lactone
-
736296
Citrus x paradisi
UDP + limonin 17-beta-D-glucoside
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Citrus x paradisi
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
-
assay at
Citrus x paradisi
Cloned(Commentary) (protein specific)
Commentary
Organism
gene SLLGT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli and in Pichia pastoris
Citrus x paradisi
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
required
Citrus x paradisi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-glucose + limonin
Citrus x paradisi
-
glucosyl-limonin + UDP
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Escherichia coli and from Pichia pastoris
Citrus x paradisi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + limonin
-
736296
Citrus x paradisi
glucosyl-limonin + UDP
-
-
-
?
UDP-glucose + limonoate A-ring-lactone
-
736296
Citrus x paradisi
UDP + limonin 17-beta-D-glucoside
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Citrus x paradisi
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
-
assay at
Citrus x paradisi
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to clade C of flavonoid and limonoid glucosyltransferases
Citrus x paradisi
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to clade C of flavonoid and limonoid glucosyltransferases
Citrus x paradisi
Other publictions for EC 2.4.1.210
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736296
Devaiah
Identification, recombinant ex ...
Citrus x paradisi
J. Agric. Food Chem.
64
1957-1969
2016
-
-
1
-
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
722071
Karim
-
Possible role of carboxyl and ...
Citrus maxima x Citrus paradisi
Chin. J. Catal.
31
1445-1451
2010
-
1
-
-
-
-
6
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
-
1
-
-
1
1
2
-
-
-
-
-
-
1
-
-
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
1
1
2
-
-
-
1
1
-
-
-
684923
Zaare-Nahandi
Delay expression of limonoid U ...
Citrus aurantium, Citrus limettioides, Citrus sinensis, Citrus unshiu, Citrus x paradisi
Biochem. Biophys. Res. Commun.
371
59-62
2008
-
5
5
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
6
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
5
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658581
Kita
Allelic structures of UDP-gluc ...
Citrus sinensis, Citrus unshiu
Euphytica
132
87-94
2003
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658786
Karim
Isolation and characterization ...
Citrus maxima, Citrus maxima Osbeck
Food Chem.
76
431-436
2002
-
-
-
-
-
-
7
2
-
3
-
-
-
2
-
-
1
-
-
1
1
1
2
-
1
1
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
3
-
-
-
-
-
1
-
1
1
1
2
-
1
1
-
-
1
1
2
-
-
-
-
-
-
-
660114
Endo
-
Modification of limonoid metab ...
Citrus sinensis
Plant Biotechnol.
19
397-403
2002
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660341
Karim
-
Preparation and properties of ...
Citrus maxima
Proc. Biochem.
38
809-814
2002
-
1
-
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
2
1
2
-
3
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
1
-
2
1
2
-
3
-
-
-
-
-
-
-
-
-
288691
Kita
Molecular cloning and characte ...
Citrus sinensis, Citrus unshiu
FEBS Lett.
469
173-178
2000
-
2
1
-
-
-
-
-
1
-
1
4
-
5
-
-
1
1
-
3
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
1
4
-
-
-
1
-
3
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288690
Sim
-
Purification of limonoid gluco ...
Citrus aurantium, Citrus limonia, Citrus maxima, Citrus sinensis, Citrus unshiu, Fragaria vesca
Phytochemistry
46
33-37
1997
-
1
-
-
-
-
1
-
-
1
2
8
-
6
-
-
1
1
-
1
1
1
9
1
-
-
-
-
1
1
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
2
8
-
-
-
1
-
1
1
1
9
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-