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Literature summary for 2.4.1.216 extracted from
- Trehalose-6-phosphate phosphorylase is part of a novel metabolic pathway for trehalose utilization in Lactococcus lactis (2001), J. Biol. Chem., 276, 42707-42713.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli and Lactococcus lactis | Lactococcus lactis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
beta-D-glucose 1-phosphate | - |
Lactococcus lactis |  |
| 4 | - |
glucose 6-phosphate | - |
Lactococcus lactis | |
| 6 | - |
trehalose 6-phosphate | - |
Lactococcus lactis | |
| 32 | - |
phosphate | - |
Lactococcus lactis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 94000 | - |
gel filtration | Lactococcus lactis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose 6-phosphate + phosphate | Lactococcus lactis | formation of trehalose 6-phosphate is favoured | glucose 6-phosphate + beta-D-glucose 1-phosphate | - |
r | |
| trehalose 6-phosphate + phosphate | Lactococcus lactis IL1403 | formation of trehalose 6-phosphate is favoured | glucose 6-phosphate + beta-D-glucose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactococcus lactis | Q9CID5 | CAA77100 | - |
| Lactococcus lactis IL1403 | Q9CID5 | CAA77100 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Lactococcus lactis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Lactococcus lactis | - |
| culture condition:trehalose-grown cell | - |
Lactococcus lactis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 32.4 | - |
- |
Lactococcus lactis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose 6-phosphate + phosphate | formation of trehalose 6-phosphate is favoured | Lactococcus lactis | glucose 6-phosphate + beta-D-glucose 1-phosphate | - |
r | |
| trehalose 6-phosphate + phosphate | formation of trehalose 6-phosphate is favoured | Lactococcus lactis IL1403 | glucose 6-phosphate + beta-D-glucose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 94000, SDS-PAGE | Lactococcus lactis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TrePP | - |
Lactococcus lactis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Lactococcus lactis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
- |
Lactococcus lactis |
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Release 2023.1 (January 2023)
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