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Literature summary for 2.4.1.25 extracted from
- Characterization of pea chloroplast D-enzyme (4-alpha-D-glucanotransferase) (1989), Plant Physiol., 91, 136-143.
General Stability
| General Stability | Organism |
|---|---|
| unstable to freezing, glycerol reduces activity and cannot be used to stabilize the enzyme at freezing temperatures | Pisum sativum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.3 | - |
maltotriose | - |
Pisum sativum |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
| chloroplast | - |
Pisum sativum | 9507 | - |
| chloroplast | - |
Arabidopsis thaliana | 9507 | - |
| cytosol | - |
Pisum sativum | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Pisum sativum |
| 98000 | - |
gel filtration | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltopentaose + maltopentaose | Escherichia coli | metabolism of starch in the bacterium | maltooligosaccharides | - |
? | |
| maltopentaose + maltopentaose | Paenibacillus macerans | metabolism of starch in the bacterium | maltooligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Bacillus subtilis | - |
- |
- |
| Bos taurus | - |
bovine | - |
| Daucus carota | - |
carrot | - |
| Escherichia coli | - |
- |
- |
| Paenibacillus macerans | - |
- |
- |
| Pisum sativum | - |
pea | - |
| Pisum sativum | - |
L. cv. Laxton's progress No. 9 | - |
| Solanum lycopersicum | - |
tomato | - |
| Solanum tuberosum | - |
potato | - |
| Spinacia oleracea | - |
spinach | - |
| Triticum aestivum | - |
wheat | - |
| Vigna radiata var. radiata | - |
- |
- |
| Zea mays | - |
sweet corn | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood | - |
Bos taurus | - |
| fruit | - |
Solanum lycopersicum | - |
| germ | - |
Triticum aestivum | - |
| kernel | - |
Zea mays | - |
| kernel | - |
Arabidopsis thaliana | - |
| leaf | - |
Spinacia oleracea | - |
| leaf | - |
Solanum tuberosum | - |
| leaf | - |
Arabidopsis thaliana | - |
| root | - |
Solanum tuberosum | - |
| root | - |
Daucus carota | - |
| stipule | - |
Pisum sativum | - |
| tuber | - |
Solanum tuberosum | - |
| tuber | - |
Arabidopsis thaliana | - |
| tuber | - |
Solanum lycopersicum | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, unstable to freezing | Pisum sativum |
| 4°C, stable for several months | Pisum sativum |
| 4°C, very stable over long periods | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltohexaose + maltotriose | - |
Pisum sativum | maltodextrins | - |
? | |
| maltopentaose + maltopentaose | metabolism of starch in the bacterium | Escherichia coli | maltooligosaccharides | - |
? | |
| maltopentaose + maltopentaose | metabolism of starch in the bacterium | Paenibacillus macerans | maltooligosaccharides | - |
? | |
| maltopentaose + maltotriose | - |
Pisum sativum | maltodextrins | - |
? | |
| maltotetraose + maltotriose | - |
Pisum sativum | maltodextrins | - |
? | |
| maltotetraose + maltotriose | - |
Solanum tuberosum | maltodextrins | - |
? | |
| maltotheptaose + maltotriose | - |
Pisum sativum | maltodextrins | - |
? | |
| maltotriose + maltotriose | - |
Pisum sativum | maltopentaose + D-glucose | - |
? | |
| maltotriose + maltotriose | - |
Arabidopsis thaliana | maltopentaose + D-glucose | - |
? | |
| maltotriose + maltotriose | - |
Pisum sativum | maltodextrins | - |
? | |
| additional information | - |
Escherichia coli | ? | - |
? | |
| additional information | - |
Arabidopsis thaliana | ? | - |
? | |
| additional information | major pathway for starch degradation in chloroplasts | Pisum sativum | ? | - |
? | |
| additional information | cannot use maltose or cyclohexaamylose as a substrate | Vigna radiata var. radiata | ? | - |
? | |
| additional information | cannot use maltose or cyclohexaamylose as a substrate | Pisum sativum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
- |
Pisum sativum |
| 5.5 | - |
- |
Daucus carota |
| 5.5 | - |
- |
Solanum lycopersicum |
| 6.5 | - |
- |
Escherichia coli |
| 6.5 | - |
- |
Arabidopsis thaliana |
| 6.7 | - |
- |
Solanum tuberosum |
| 7.5 | 8 | - |
Pisum sativum |
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