Activating Compound | Comment | Organism | Structure |
---|---|---|---|
maltoheptaose | stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 124% | Thermotoga maritima | |
maltohexaose | stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 144% | Thermotoga maritima | |
maltopentaose | stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 210% | Thermotoga maritima | |
maltose | stimulating effect of maltooligosaccharides on the conversion of amylose, relative activity 259% | Thermotoga maritima | |
maltotetraose | stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 246% | Thermotoga maritima | |
maltotriose | stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 210% | Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
chromosomal gene cloned and expressed in Escherichia coli and Corynebacterium glutamicum | Thermotoga maritima |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Thermotoga maritima | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
SDS-PAGE | Thermotoga maritima |
56000 | - |
size exclusion chromatography | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltopentaose + maltopentaose | Bacillus sp. (in: Bacteria) | metabolism of starch in the bacterium | maltooligosaccharides | - |
? | |
maltopentaose + maltopentaose | Thermotoga maritima | metabolism of starch in the bacterium | maltooligosaccharides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Thermotoga maritima | - |
MSB8 | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
MSB8 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + maltopentaose | - |
Thermotoga maritima | maltooligosaccharides | - |
? | |
amylose + maltopentaose | - |
Thermotoga maritima | maltooligosaccharides | - |
? | |
maltoheptaose + maltopentaose | - |
Bacillus sp. (in: Bacteria) | maltooligosaccharides | - |
? | |
maltoheptaose + maltopentaose | - |
Thermotoga maritima | maltooligosaccharides | - |
? | |
maltohexaose + maltopentaose | - |
Bacillus sp. (in: Bacteria) | maltooligosaccharides | - |
? | |
maltohexaose + maltopentaose | - |
Thermotoga maritima | maltooligosaccharides | - |
? | |
maltopentaose + maltopentaose | - |
Bacillus sp. (in: Bacteria) | new oligosaccharides | - |
? | |
maltopentaose + maltopentaose | - |
Thermotoga maritima | new oligosaccharides | - |
? | |
maltopentaose + maltopentaose | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | new oligosaccharides | - |
? | |
maltopentaose + maltopentaose | metabolism of starch in the bacterium | Bacillus sp. (in: Bacteria) | maltooligosaccharides | - |
? | |
maltopentaose + maltopentaose | metabolism of starch in the bacterium | Thermotoga maritima | maltooligosaccharides | - |
? | |
maltotetraose + maltopentaose | - |
Thermotoga maritima | maltooligosaccharides | - |
? | |
maltotetraose + maltopentaose | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | maltooligosaccharides | - |
? | |
additional information | maltose and maltotriose are not disproportionated, glucose does not function as an acceptor sugar in transfer reactions, glucose also never appears as a reaction product | Thermotoga maritima | ? | - |
? | |
additional information | does not use maltose, maltotriose or maltotetraose as acceptor substrates in maltodextrinyltransfer reactions | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | alpha-D-glucose, beta-D-fructose, D-ribose, D-arabinose, D-xylose, isomaltose, D-trehalose, D-cellobiose, lactose, sucrose, raffinose and N-acetyl-D-glucosamine cannot participate as acceptor sugars in glucanosyl transfer from amylose | Thermotoga maritima | ? | - |
? | |
additional information | maltose and maltotriose are not disproportionated, glucose does not function as an acceptor sugar in transfer reactions, glucose also never appears as a reaction product | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
additional information | alpha-D-glucose, beta-D-fructose, D-ribose, D-arabinose, D-xylose, isomaltose, D-trehalose, D-cellobiose, lactose, sucrose, raffinose and N-acetyl-D-glucosamine cannot participate as acceptor sugars in glucanosyl transfer from amylose | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
starch + maltopentaose | - |
Bacillus sp. (in: Bacteria) | alpha-1,4-D-glucans | - |
? | |
starch + maltopentaose | starch can serve as acceptor molecule in glycosyl transfer reactions | Thermotoga maritima | alpha-1,4-D-glucans | - |
? | |
starch + maltopentaose | starch can serve as acceptor molecule in glycosyl transfer reactions | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | alpha-1,4-D-glucans | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 53000, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
GTase | - |
Thermotoga maritima |
maltodextrin glycosyltransferase | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | - |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 80 | stable at 37°C, retains more than 90% of its maximum activity between 55-80°C, half-life of activity of about 3 h at 80°C | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 10 | - |
Thermotoga maritima |