Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.25 extracted from

  • Liebl, W.; Feil, R.; Gabelsberger, J.; Kellermann, J.; Schleifer, K.H.
    Purification and characterization of a novel thermostable 4-alpha-glucanotransferase of Thermotoga maritima cloned in Escherichia coli (1992), Eur. J. Biochem., 207, 81-88.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
maltoheptaose stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 124% Thermotoga maritima
maltohexaose stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 144% Thermotoga maritima
maltopentaose stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 210% Thermotoga maritima
maltose stimulating effect of maltooligosaccharides on the conversion of amylose, relative activity 259% Thermotoga maritima
maltotetraose stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 246% Thermotoga maritima
maltotriose stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 210% Thermotoga maritima

Cloned(Commentary)

Cloned (Comment) Organism
chromosomal gene cloned and expressed in Escherichia coli and Corynebacterium glutamicum Thermotoga maritima

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Thermotoga maritima 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
SDS-PAGE Thermotoga maritima
56000
-
size exclusion chromatography Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
maltopentaose + maltopentaose Bacillus sp. (in: Bacteria) metabolism of starch in the bacterium maltooligosaccharides
-
?
maltopentaose + maltopentaose Thermotoga maritima metabolism of starch in the bacterium maltooligosaccharides
-
?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Thermotoga maritima
-
MSB8
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
MSB8
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + maltopentaose
-
Thermotoga maritima maltooligosaccharides
-
?
amylose + maltopentaose
-
Thermotoga maritima maltooligosaccharides
-
?
maltoheptaose + maltopentaose
-
Bacillus sp. (in: Bacteria) maltooligosaccharides
-
?
maltoheptaose + maltopentaose
-
Thermotoga maritima maltooligosaccharides
-
?
maltohexaose + maltopentaose
-
Bacillus sp. (in: Bacteria) maltooligosaccharides
-
?
maltohexaose + maltopentaose
-
Thermotoga maritima maltooligosaccharides
-
?
maltopentaose + maltopentaose
-
Bacillus sp. (in: Bacteria) new oligosaccharides
-
?
maltopentaose + maltopentaose
-
Thermotoga maritima new oligosaccharides
-
?
maltopentaose + maltopentaose
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 new oligosaccharides
-
?
maltopentaose + maltopentaose metabolism of starch in the bacterium Bacillus sp. (in: Bacteria) maltooligosaccharides
-
?
maltopentaose + maltopentaose metabolism of starch in the bacterium Thermotoga maritima maltooligosaccharides
-
?
maltotetraose + maltopentaose
-
Thermotoga maritima maltooligosaccharides
-
?
maltotetraose + maltopentaose
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 maltooligosaccharides
-
?
additional information maltose and maltotriose are not disproportionated, glucose does not function as an acceptor sugar in transfer reactions, glucose also never appears as a reaction product Thermotoga maritima ?
-
?
additional information does not use maltose, maltotriose or maltotetraose as acceptor substrates in maltodextrinyltransfer reactions Bacillus sp. (in: Bacteria) ?
-
?
additional information alpha-D-glucose, beta-D-fructose, D-ribose, D-arabinose, D-xylose, isomaltose, D-trehalose, D-cellobiose, lactose, sucrose, raffinose and N-acetyl-D-glucosamine cannot participate as acceptor sugars in glucanosyl transfer from amylose Thermotoga maritima ?
-
?
additional information maltose and maltotriose are not disproportionated, glucose does not function as an acceptor sugar in transfer reactions, glucose also never appears as a reaction product Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
?
additional information alpha-D-glucose, beta-D-fructose, D-ribose, D-arabinose, D-xylose, isomaltose, D-trehalose, D-cellobiose, lactose, sucrose, raffinose and N-acetyl-D-glucosamine cannot participate as acceptor sugars in glucanosyl transfer from amylose Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
?
starch + maltopentaose
-
Bacillus sp. (in: Bacteria) alpha-1,4-D-glucans
-
?
starch + maltopentaose starch can serve as acceptor molecule in glycosyl transfer reactions Thermotoga maritima alpha-1,4-D-glucans
-
?
starch + maltopentaose starch can serve as acceptor molecule in glycosyl transfer reactions Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 alpha-1,4-D-glucans
-
?

Subunits

Subunits Comment Organism
monomer 1 * 53000, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
GTase
-
Thermotoga maritima
maltodextrin glycosyltransferase
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 90
-
Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 80 stable at 37°C, retains more than 90% of its maximum activity between 55-80°C, half-life of activity of about 3 h at 80°C Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
3.5 10
-
Thermotoga maritima