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Literature summary for 2.4.1.25 extracted from
- Expression and characterization of 4-alpha-glucanotransferase genes from Manihot esculenta Crantz and Arabidopsis thaliana and their use for the production of cycloamyloses (2014), Process Biochem., 49, 84-89.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Star (DE3) One Shot cells | Manihot esculenta |
| expressed in Escherichia coli BL21 Star (DE3) One Shot cells | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 27.5 | - |
maltotriose | at pH 7.0 and 37 °C | Arabidopsis thaliana |  |
| 69.2 | - |
maltotriose | at pH 7.0 and 37 °C | Manihot esculenta | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59400 | - |
2 * 59400, calculated from amino acid sequence | Manihot esculenta |
| 66000 | - |
2 * 66000, SDS-PAGE | Arabidopsis thaliana |
| 117000 | - |
gel filtration | Manihot esculenta |
| 123000 | - |
gel filtration | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9LV91 | - |
- |
| Manihot esculenta | - |
cultivar KU50 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni HiTrap column chromatography and Superdex 75 gel filtration | Manihot esculenta |
| Ni HiTrap column chromatography and Superdex 75 gel filtration | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltotriose + maltotriose | - |
Manihot esculenta | maltooligosaccharides | - |
? | |
| maltotriose + maltotriose | - |
Arabidopsis thaliana | maltooligosaccharides | - |
? | |
| potato amylose + glycosyl acceptor | - |
Manihot esculenta | cycloamylose | the enzyme can produce cycloamyloses in the range 16 to more than 60 glucose residues | ? | |
| potato amylose + glycosyl acceptor | - |
Arabidopsis thaliana | cycloamylose | the enzyme can produce cycloamyloses in the range 1650 glucose residues | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 66000, SDS-PAGE | Arabidopsis thaliana |
| homodimer | 2 * 59400, calculated from amino acid sequence | Manihot esculenta |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4alphaGTase | - |
Manihot esculenta |
| 4alphaGTase | - |
Arabidopsis thaliana |
| DPE1 | - |
Manihot esculenta |
| DPE1 | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Manihot esculenta |
| 37 | - |
- |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 580 | - |
maltotriose | at pH 7.0 and 37 °C | Arabidopsis thaliana | |
| 894 | - |
maltotriose | at pH 7.0 and 37 °C | Manihot esculenta | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Manihot esculenta |
| 6 | 8 | - |
Arabidopsis thaliana |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Manihot esculenta | calculated from amino acid sequence | - |
5.1 |
| Arabidopsis thaliana | calculated from amino acid sequence | - |
5.1 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.9 | - |
maltotriose | at pH 7.0 and 37 °C | Manihot esculenta | |
| 21.6 | - |
maltotriose | at pH 7.0 and 37 °C | Arabidopsis thaliana | |
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