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Literature summary for 2.4.1.255 extracted from

  • Lazarus, B.D.; Roos, M.D.; Hanover, J.A.
    Mutational analysis of the catalytic domain of O-linked N-acetylglucosaminyl transferase (2005), J. Biol. Chem., 280, 35537-35544.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol increases the activity of wild type OGT Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
C836S site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, 10% activity compared to wild type Homo sapiens
C839S site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, less than 1% activity compared to wild type Homo sapiens
D407A significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
D422A site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I, produces a 50-100% increase in activity Homo sapiens
D438A significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
D488A significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
D505A significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
D549A significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
D554A significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
E482A significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
E556A significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
E568A significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
F439A significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
F460A significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
F721A site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, 10% activity compared to wild type Homo sapiens
F752A site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, 110% activity compared to wild type Homo sapiens
F776A site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, 10% activity compared to wild type Homo sapiens
G402S significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
G453S significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
G472A significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
G538S significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
L796A site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, less than 1% activity compared to wild type Homo sapiens
additional information following mutations are not included for enzymatic analysis, because not sufficient amounts of protein could be produced: Y387A, F439A, D505A, W536A, Y539A, D549A, D554A, and E556A Homo sapiens
additional information site-directed mutagenesis to target potentially important amino acid residues within the two conserved catalytic domains of OGT (CD I and CD II), followed by an in vitro glycosylation assay to evaluate N-acetylglucosaminyltransferase activity after bacterial expression Homo sapiens
W536A significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
W735A site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, less than 1% activity compared to wild type Homo sapiens
W748A site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, less than 1% activity compared to wild type Homo sapiens
W812A site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, less than 1% activity compared to wild type Homo sapiens
W878A site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, less than 1% activity compared to wild type Homo sapiens
Y387A significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I, mutation is not included for enzymatic analysis, because not sufficient amounts of protein could be produced Homo sapiens
Y434A site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens
Y539A significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
alloxan
-
Homo sapiens
N-ethylmaleimide
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
UDP-GlcNAc F752A mutant Homo sapiens
0.29
-
UDP-GlcNAc D422A mutant Homo sapiens
0.44
-
UDP-GlcNAc F776A mutant Homo sapiens
0.8
-
UDP-GlcNAc mOGT wild type Homo sapiens
1.9
-
UDP-GlcNAc Y434A mutant, approximately double the Km value of wild type Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens catalyzes the transfer of O-linked GlcNAc to serine/threonine residues of a variety of target proteins, many of which have been implicated in such diseases as diabetes and neurodegeneration ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information catalyzes the transfer of O-linked GlcNAc to serine/threonine residues of a variety of target proteins, many of which have been implicated in such diseases as diabetes and neurodegeneration Homo sapiens ?
-
?
UDP-GlcNAc + Nup62 protein
-
Homo sapiens UDP + N-acetyl-D-glucosmainyl-[Nup62 protein]
-
?

Synonyms

Synonyms Comment Organism
mOGT mitochondrial OGT Homo sapiens
O-linked N-acetylglucosaminyltransferase
-
Homo sapiens
OGT
-
Homo sapiens