Literature summary for 2.4.1.269 extracted from

Flint, J.; Taylor, E.; Yang, M.; Bolam, D.N.; Tailford, L.E.; Martinez-Fleites, C.; Dodson, E.J.; Davis, B.G.; Gilbert, H.J.; Davies, G.J.
Structural dissection and high-throughput screening of mannosylglycerate synthase (2005), Nat. Struct. Mol. Biol., 12, 608-614.

Search for more literature for 2.4.1.269

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop method	Rhodothermus marinus

Protein Variants

Protein Variants	Comment	Organism
D100A	inactive mutant enzyme	Rhodothermus marinus
D102A	inactive mutant enzyme	Rhodothermus marinus
H217A	inactive mutant enzyme	Rhodothermus marinus
R131A	inactive mutant enzyme	Rhodothermus marinus

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	-	Rhodothermus marinus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
21.3	-	D-lactate	pH 7.8, 25°C, cosubstrate: GDP-mannose	Rhodothermus marinus
23.7	-	glycolic acid	pH 7.8, 25°C, cosubstrate: GDP-mannose	Rhodothermus marinus
81.2	-	GDP-mannose	pH 7.8, 25°C, cosubstrate: D-glycerate	Rhodothermus marinus
89.4	-	GDP-mannose	pH 7.8, 65°C, cosubstrate: D-glycerate	Rhodothermus marinus
96.5	-	D-glycerate	pH 7.8, 65°C, cosubstrate: GDP-mannose	Rhodothermus marinus
121.9	-	D-glycerate	pH 7.8, 25°C, cosubstrate: GDP-mannose	Rhodothermus marinus
124.9	-	GDP-glucose	pH 7.8, 25°C, cosubstrate: D-glycerate	Rhodothermus marinus
138.6	-	GDP-glucose	pH 7.8, 25°C, cosubstrate: D-glycerate	Rhodothermus marinus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	absolute requirement for divalent metal ions. 200 mM of Mg2+, Ca2+, Mn2+, Ni2+ and Co2+ giving relative rates of 1.0, 0.6, 0.33, 0.16 and 0.15, respectively	Rhodothermus marinus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GDP-alpha-D-glucose + D-glycerate	Rhodothermus marinus	the enzyme catalyzes the formation of the stress protectant 2-O-alpha-D-mannosyl glycerate	2-O-(alpha-D-glucopyranosyl)-D-glycerate + GDP	-	?

Organism

Organism	UniProt	Comment	Textmining
Rhodothermus marinus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GDP-alpha-D-glucose + D-glycerate	the enzyme catalyzes the formation of the stress protectant 2-O-alpha-D-mannosyl glycerate	Rhodothermus marinus	2-O-(alpha-D-glucopyranosyl)-D-glycerate + GDP	-	?
GDP-alpha-D-glucose + D-glycerate	at 37°C mannosylglycerate synthase shows donor flexibility and can use alpha-GDP-D-Man, alpha-GDP-D-Glc, beta-GDP-L-fucose, alpha-UDP-D-Man and alpha-UDP-D-Glc, but not Man-1-P, as substrates. The kcat/KM-value for GDP-glucose is 2.5fold lower than the value for GDP-mannose at 25°C. The kcat/KM-value for GDP-glucose is 8.6fold lower than the value for GDP-mannose at 65°C	Rhodothermus marinus	2-O-(alpha-D-glucopyranosyl)-D-glycerate + GDP	-	?
GDP-mannose + D-glycerate	at 37°C mannosylglycerate synthase shows donor flexibility and can use alpha-GDP-D-Man, alpha-GDP-D-Glc, beta-GDP-L-fucose, alpha-UDP-D-Man and alpha-UDP-D-Glc, but not Man-1-P, as substrates. The kcat/KM-value for GDP-glucose is 2.5fold lower than the value for GDP-mannose at 25°C. The kcat/KM-value for GDP-glucose is 8.6fold lower than the value for GDP-mannose at 65°C	Rhodothermus marinus	2-O-(alpha-D-mannopyranosyl)-D-glycerate + GDP	-	?
GDP-mannose + D-lactate	the kcat/KM-value for D-lactate is 70fold lower than the value for D-glycerate at 25°C	Rhodothermus marinus	?	-	?
GDP-mannose + glycolic acid	the kcat/KM-value for glycolic acid is 70fold lower than the value for glycolic acid at 25°C	Rhodothermus marinus	?	-	?

Synonyms

Synonyms	Comment	Organism
MGS	-	Rhodothermus marinus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Rhodothermus marinus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0026	-	D-lactate	pH 7.8, 25°C, cosubstrate: GDP-mannose	Rhodothermus marinus
0.0052	-	glycolic acid	pH 7.8, 25°C, cosubstrate: GDP-mannose	Rhodothermus marinus
0.63	-	GDP-glucose	pH 7.8, 25°C, cosubstrate: D-glycerate	Rhodothermus marinus
1.02	-	GDP-mannose	pH 7.8, 25°C, cosubstrate: D-glycerate	Rhodothermus marinus
1.02	-	D-glycerate	pH 7.8, 25°C, cosubstrate: GDP-mannose	Rhodothermus marinus
1.1	-	GDP-glucose	pH 7.8, 25°C, cosubstrate: D-glycerate	Rhodothermus marinus
6.1	-	GDP-mannose	pH 7.8, 65°C, cosubstrate: D-glycerate	Rhodothermus marinus
6.5	-	D-glycerate	pH 7.8, 65°C, cosubstrate: GDP-mannose	Rhodothermus marinus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	assay at	Rhodothermus marinus

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Release 2023.1 (January 2023)