Literature summary for 2.4.1.281 extracted from

Senoura, T.; Ito, S.; Taguchi, H.; Higa, M.; Hamada, S.; Matsui, H.; Ozawa, T.; Jin, S.; Watanabe, J.; Wasaki, J.; Ito, S.
New microbial mannan catabolic pathway that involves a novel mannosylglucose phosphorylase (2011), Biochem. Biophys. Res. Commun., 408, 701-716.

Search for more literature for 2.4.1.281

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacteroides fragilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2	-	phosphate	pH 7.5, 30°C	Bacteroides fragilis
8.3	-	4-O-beta-D-mannopyranosyl-D-glucopyranose	pH 7.5, 30°C	Bacteroides fragilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Bacteroides fragilis	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
43890	-	calculated from amino acid sequence	Bacteroides fragilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate	Bacteroides fragilis	phosphorolysis reaction	D-glucose + alpha-D-mannose 1-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Bacteroides fragilis	Q5LH68	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
28.5	-	pH 7.5, 30°C	Bacteroides fragilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate	phosphorolysis reaction	Bacteroides fragilis	D-glucose + alpha-D-mannose 1-phosphate	-	r
additional information	no substrates are 4-O-beta-D-xylopyranosyl-D-xylose, sophorose, sucrose, laminaribiose, cellobiose, lactose, epilactose, 4-O-beta-D-mannopyranosyl-D-mannose, gentiobiose, kojibiose, nigerose, maltose, isomaltose.	Bacteroides fragilis	?	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Bacteroides fragilis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
24.8	-	phosphate	pH 7.5, 30°C	Bacteroides fragilis
42.2	-	4-O-beta-D-mannopyranosyl-D-glucopyranose	pH 7.5, 30°C	Bacteroides fragilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Bacteroides fragilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
5.1	-	4-O-beta-D-mannopyranosyl-D-glucopyranose	pH 7.5, 30°C	Bacteroides fragilis
12.4	-	phosphate	pH 7.5, 30°C	Bacteroides fragilis

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Release 2023.1 (January 2023)