Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) Quarters cells | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0038 | - |
UDP-alpha-D-galactofuranose | apparent value, spectrophotometric assay, in 50 mM MOPS, pH 7.9, 50 mM KCl, 20 mM MgCl2, at 37°C | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73500 | - |
x * 73500, His-tagged enzyme, estimated from SDS-PAGE | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA agarose column chromatography and Sephacryl S-100 gel filtration | Mycobacterium tuberculosis |
Storage Stability | Organism |
---|---|
-80°C, purified enzyme with 20% (v/v) glycerol, four months, no significant loss of activity | Mycobacterium tuberculosis |
4°C, purified enzyme with 20% (v/v) glycerol, 2 weeks, no significant loss of activity | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | the enzyme adds the third and subsequent alpha-D-galactofuranosyl residues to the growing galactan chain | Mycobacterium tuberculosis | UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
UDP-alpha-D-galactofuranose + beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-O(CH2)7CH3 | - |
Mycobacterium tuberculosis | UDP + ? | - |
? | |
UDP-alpha-D-galactofuranose + beta-D-Galf-(1->6)-beta-D-Galf-(1->5)-beta-D-Galf-O(CH2)7CH3 | - |
Mycobacterium tuberculosis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 73500, His-tagged enzyme, estimated from SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
galactofuranosyltransferase | - |
Mycobacterium tuberculosis |
GlfT2 | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.2 | - |
UDP-alpha-D-galactofuranose | spectrophotometric assay, in 50 mM MOPS, pH 7.9, 50 mM KCl, 20 mM MgCl2, at 37°C | Mycobacterium tuberculosis |