Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.293 extracted from

  • Glover, K.J.; Weerapana, E.; Imperiali, B.
    In vitro assembly of the undecaprenylpyrophosphate-linked heptasaccharide for prokaryotic N-linked glycosylation (2005), Proc. Natl. Acad. Sci. USA, 102, 14255-14259.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.293

Activating Compound

Activating Compound Comment Organism Structure
additional information PglI prefers native lipids for optimal activity Campylobacter jejuni

Cloned(Commentary)

Cloned (Comment) Organism
gene pglI, overexpression of His-tagged PglI in Escherichia coli Campylobacter jejuni

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Campylobacter jejuni 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 x * 36000, recombinant His-tagged Pgli, SDS-PAGE Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol Campylobacter jejuni
-
 UDP + [GalNAc-alpha-(1->4)]2-[Glc-beta-(1->3)]-[GalNAc-alpha-(1->4)]2-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol
-
 ?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
 gene pglI, pgl gene cluster
-
Campylobacter jejuni NCTC 11168
-
 gene pglI, pgl gene cluster
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged PglI from Escherichia coli by nickel affinity chromatography Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol
-
 Campylobacter jejuni UDP + [GalNAc-alpha-(1->4)]2-[Glc-beta-(1->3)]-[GalNAc-alpha-(1->4)]2-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol
-
 ?

Subunits

Subunits Comment Organism
? x * 36000, recombinant His-tagged Pgli, SDS-PAGE Campylobacter jejuni

Synonyms

Synonyms Comment Organism
PglI
-
 Campylobacter jejuni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 assay at Campylobacter jejuni

General Information

General Information Comment Organism
metabolism PglI is part of a general N-linked glycosylation pathway, encoded by the pgl gene cluster, which culminates in the transfer of a heptasaccharide: GalNAc-alpha1,4-GalNAc-alpha1,4-(Glcbeta1,3)-GalNAc-alpha1,4-GalNAc-alpha1,4-GalNAc-alpha1,3-Bac, where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose), from a membrane-anchored undecaprenyldiphosphate-linked donor to the asparagine side chain of proteins at the Asn-X-Ser/Thr motif. The glycosyltransferases, PglA, PglH, PglI, and PglJ, responsible for the biosynthesis of the undecaprenyl diphoshate-linked heptasaccharide. The final branching glucose is added by PglI, and the flippase, WlaB, then flips the completed lipid-linked heptasaccharide into the periplasm Campylobacter jejuni