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Literature summary for 2.4.1.298 extracted from

  • Yamazaki, M.; Gong, Z.; Fukuchi-Mizutani, M.; Fukui, Y.; Tanaka, Y.; Kusumi, T.; Saito, K.
    Molecular cloning and biochemical characterization of a novel anthocyanin 5-O-glucosyltransferase by mRNA differential display for plant forms regarding anthocyanin (1999), J. Biol. Chem., 274, 7405-7411.
    View publication on PubMed
Search for more literature for 2.4.1.298

Activating Compound

Activating Compound Comment Organism Structure
EDTA 25% activation at 1 mM Perilla frutescens

Cloned(Commentary)

Cloned (Comment) Organism
gene HGT8, DNA and amino acid sequence determination and analysis, sequence comparison, expression in Saccharomyces cerevisiae Glandularia x hybrida
gene PF3R4, DNA and amino acid sequence determination and analysis, sequence comparison, expression in Saccharomyces cerevisiae Perilla frutescens
gene PF3R6, DNA and amino acid sequence determination and analysis, sequence comparison, expression in Saccharomyces cerevisiae Perilla frutescens

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate complete inhibition at 1 mM Perilla frutescens
Co2+ 43% inhibition at 1 mM Perilla frutescens
Mn2+ 41% inhibition at 1 mM Perilla frutescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0314
-
 cyanidin 3-O-beta-D-glucoside pH 8.5, 30°C, recombinant enzyme Perilla frutescens
0.94
-
 UDP-alpha-D-glucose pH 8.5, 30°C, recombinant enzyme Perilla frutescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49109
-
 x * 49109, sequence calculation Perilla frutescens
50973
-
 x * 50973, sequence calculation Perilla frutescens
51347
-
 x * 51347, sequence calculation Glandularia x hybrida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-(4-coumaroyl)-beta-D-glucoside) Perilla frutescens low activity UDP + cyanidin 3-O-(6-O-(4-coumaroyl)-beta-D-glucosyl)-5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-(4-coumaroyl)-beta-D-glucoside) Glandularia x hybrida low activity UDP + cyanidin 3-O-(6-O-(4-coumaroyl)-beta-D-glucosyl)-5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-(4-coumaroyl)-beta-D-glucoside) Perilla frutescens Chirimenjiso Shikun low activity UDP + cyanidin 3-O-(6-O-(4-coumaroyl)-beta-D-glucosyl)-5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside Perilla frutescens best substrate UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside Glandularia x hybrida best substrate UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside Perilla frutescens Chirimenjiso Shikun best substrate UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?

Organism

Organism UniProt Comment Textmining
Glandularia x hybrida Q9ZR25 gene HGT8
-
Perilla frutescens Q9ZR26 gene PF3R6; var. crispa, reddish-purple, gene PF3R6, no activity in the green/cv. Chirimen-aojiso Seikun form
-
Perilla frutescens Q9ZR27 gene PF3R4; var. crispa, reddish-purple, gene PF3R4, no activity in the green/cv. Chirimen-aojiso Seikun form
-
Perilla frutescens Chirimenjiso Shikun Q9ZR26 gene PF3R6; var. crispa, reddish-purple, gene PF3R6, no activity in the green/cv. Chirimen-aojiso Seikun form
-
Perilla frutescens Chirimenjiso Shikun Q9ZR27 gene PF3R4; var. crispa, reddish-purple, gene PF3R4, no activity in the green/cv. Chirimen-aojiso Seikun form
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Perilla frutescens
-
leaf
-
 Glandularia x hybrida
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity, overview. No activity with cyanidin Perilla frutescens ?
-
 ?
additional information the enzyme encoded by gene PF3R6 is inactive Perilla frutescens ?
-
 ?
additional information the enzyme encoded by gene PF3R6 is inactive Perilla frutescens Chirimenjiso Shikun ?
-
 ?
additional information substrate specificity, overview. No activity with cyanidin Perilla frutescens Chirimenjiso Shikun ?
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-(6''-4-coumaroyl)-beta-D-glucoside low activity Perilla frutescens UDP + cyanidin 3-O-(6''-4-coumaroyl)-glucoside 5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-(6''-caffeoyl)-beta-D-glucoside low activity Perilla frutescens UDP + cyanidin 3-O-(6''-caffeoyl)-glucoside 5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-(4-coumaroyl)-beta-D-glucoside) low activity Perilla frutescens UDP + cyanidin 3-O-(6-O-(4-coumaroyl)-beta-D-glucosyl)-5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-(4-coumaroyl)-beta-D-glucoside) low activity Glandularia x hybrida UDP + cyanidin 3-O-(6-O-(4-coumaroyl)-beta-D-glucosyl)-5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-(4-coumaroyl)-beta-D-glucoside) low activity Perilla frutescens Chirimenjiso Shikun UDP + cyanidin 3-O-(6-O-(4-coumaroyl)-beta-D-glucosyl)-5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside best substrate Perilla frutescens UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside best substrate Glandularia x hybrida UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside best substrate Perilla frutescens Chirimenjiso Shikun UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside
-
 Perilla frutescens UDP + delphinidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside
-
 Perilla frutescens Chirimenjiso Shikun UDP + delphinidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside
-
 Perilla frutescens UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside
-
 Perilla frutescens Chirimenjiso Shikun UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
 ?

Subunits

Subunits Comment Organism
? x * 49109, sequence calculation Perilla frutescens
? x * 50973, sequence calculation Perilla frutescens
? x * 51347, sequence calculation Glandularia x hybrida

Synonyms

Synonyms Comment Organism
5-GT
-
 Perilla frutescens
5-GT
-
 Glandularia x hybrida
anthocyanin 5-O-glucosyltransferase
-
 Perilla frutescens
anthocyanin 5-O-glucosyltransferase
-
 Glandularia x hybrida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Perilla frutescens
30
-
 assay at Glandularia x hybrida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 assay at Perilla frutescens
8.5
-
 assay at Perilla frutescens
8.5
-
 assay at Glandularia x hybrida