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Literature summary for 2.4.1.319 extracted from

  • Kawahara, R.; Saburi, W.; Odaka, R.; Taguchi, H.; Ito, S.; Mori, H.; Matsui, H.
    Metabolic mechanism of mannan in a ruminal bacterium, Ruminococcus albus, involving two mannoside phosphorylases and cellobiose 2-epimerase: discovery of a new carbohydrate phosphorylase, beta-1,4-mannooligosaccharide phosphorylase (2012), J. Biol. Chem., 287, 42389-42399.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.319

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Ruminococcus albus
expression in Escherichia coli BL21 (DE3) Ruminococcus albus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.21
-
 [(1->4)-beta-D-mannosyl]4 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
3.21
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
4.48
-
 [(1->4)-beta-D-mannosyl]6 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
4.48
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
4.55
-
 [(1->4)-beta-D-mannosyl]5 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
4.55
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
5.29
-
 [(1->4)-beta-D-mannosyl]5 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
5.29
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
7.59
-
 [(1->4)-beta-D-mannosyl]4 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
7.59
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
7.94
-
 [(1->4)-beta-D-mannosyl]3 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
7.94
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
13.3
-
 [(1->4)-beta-D-mannosyl]3 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
13.3
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
28.4
-
 4-O-beta-D-mannosyl-D-glucose pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
30.7
-
 [(1->4)-beta-D-mannosyl]2 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
30.7
-
 beta-D-mannopyranosyl-(1->4)-D-mannopyranose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
44.5
-
 [(1->4)-beta-D-mannosyl]2 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
44.5
-
 beta-D-mannopyranosyl-(1->4)-D-mannopyranose pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Ruminococcus albus 5737
-
intracellular
-
 Ruminococcus albus 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37823
-
 6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence Ruminococcus albus
38000
-
 6 * 38000, SDS-PAGE Ruminococcus albus
38000
-
 6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence Ruminococcus albus
209000
-
 gel filtration Ruminococcus albus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[(1->4)-beta-D-mannosyl]n + phosphate Ruminococcus albus
-
 [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
-
 r
[(1->4)-beta-D-mannosyl]n + phosphate Ruminococcus albus the enzyme is involved in the metabolic pathway of mannan [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Ruminococcus albus E6UBR9
-
-
Ruminococcus albus NE1 E6UBR9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ruminococcus albus
recombinant enzyme Ruminococcus albus

Reaction

Reaction Comment Organism Reaction ID
[(1->4)-beta-D-mannosyl]n + phosphate = [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate n>=3, sequential bi-bi mechanism Ruminococcus albus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.03
-
 pH 6.5, 37°C Ruminococcus albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-beta-D-mannosyl-D-glucose + phosphate
-
 Ruminococcus albus D-glucose + alpha-D-mannosyl 1-phosphate
-
 ?
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
-
 r
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
-
 r
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
-
 Ruminococcus albus beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate
-
 r
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate best substrate for the synthetic reaction Ruminococcus albus beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
-
 r
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
-
 Ruminococcus albus beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate
-
 r
beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
-
 r
beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
-
 Ruminococcus albus beta-D-mannopyranosyl-(1->4)-D-mannopyranose + alpha-D-mannose 1-phosphate
-
 r
beta-D-mannopyranosyl-(1->4)-beta-D-mannopyranosyl-(1->4)-D-mannopyranose + phosphate
-
 Ruminococcus albus beta-D-mannopyranosyl-(1->4)-D-mannopyranose + alpha-D-mannose 1-phosphate
-
 r
beta-D-mannopyranosyl-(1->4)-D-mannopyranose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus beta-D-mannopyranosyl-(1->4)-beta-D-mannopyranosyl-(1->4)-D-mannopyranose + phosphate
-
 r
beta-D-mannopyranosyl-(1->4)-D-mannopyranose + phosphate
-
 Ruminococcus albus D-mannose + alpha-D-mannose 1-phosphate
-
 r
additional information The enzyme also catalyzes the reaction of EC 2.4.1.281, 4-O-beta-D-mannosyl-D-glucose phosphorylase Ruminococcus albus ?
-
 ?
[(1->4)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]3 + phosphate
-
 ?
[(1->4)-beta-D-mannosyl]2 + phosphate
-
 Ruminococcus albus D-mannose + alpha-D-mannose 1-phosphate
-
 ?
[(1->4)-beta-D-mannosyl]3 + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]4 + phosphate
-
 ?
[(1->4)-beta-D-mannosyl]3 + phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate
-
 ?
[(1->4)-beta-D-mannosyl]4 + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]5 + phosphate
-
 ?
[(1->4)-beta-D-mannosyl]4 + phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]3 + alpha-D-mannose 1-phosphate
-
 ?
[(1->4)-beta-D-mannosyl]5 + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]6 + phosphate
-
 ?
[(1->4)-beta-D-mannosyl]5 + phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]4 + alpha-D-mannose 1-phosphate
-
 ?
[(1->4)-beta-D-mannosyl]6 + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]7 + phosphate
-
 ?
[(1->4)-beta-D-mannosyl]6 + phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]5 + alpha-D-mannose 1-phosphate
-
 ?
[(1->4)-beta-D-mannosyl]n + phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
-
 r
[(1->4)-beta-D-mannosyl]n + phosphate the enzyme is involved in the metabolic pathway of mannan Ruminococcus albus [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
-
 r
[(1->4)-beta-D-mannosyl]n + phosphate the enzyme catalyses the reversible phosphorolysis of beta-1,4-mannooligosaccharide with a minimum size of three monomers. It acts on D-glucose derivatives at the C2- and C3-positions, including deoxy- and deoxyfluoro-analogues and epimers, but not on those substituted at the C6-position. It shows high synthetic activity toward the following oligosaccharides: beta-linked glucobioses, maltose, N,N'-diacetylchitobiose, and beta-1,4-mannooligosaccharides. Particularly, beta-1,4-mannooligosaccharides serve as significantly better acceptor substrates for the enzyme than D-glucose. In the phosphorolytic reactions, RaMP2 has weak activity toward beta-1,4-mannobiose but efficiently degrades beta-1,4-mannooligosaccharides longer than beta-1,4-mannobiose. It is thought to catalyze the phosphorolysis of beta-1,4-mannooligosaccharides longer than beta-1,4-mannobiose to produce alpha-D-mannose 1-phosphate and beta-1,4-mannobiose Ruminococcus albus [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
-
 r
[(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate the enzyme catalyses the reversible phosphorolysis of beta-1,4-mannooligosaccharide with a minimum size of three monomers. It acts on D-glucose derivatives at the C2- and C3-positions, including deoxy- and deoxyfluoro-analogues and epimers, but not on those substituted at the C6-position. It shows high synthetic activity toward the following oligosaccharides: beta-linked glucobioses, maltose, N,N'-diacetylchitobiose, and beta-1,4-mannooligosaccharides. Particularly, beta-1,4-mannooligosaccharides serve as significantly better acceptor substrates for the enzyme than D-glucose. In the phosphorolytic reactions, RaMP2 has weak activity toward beta-1,4-mannobiose but efficiently degrades beta-1,4-mannooligosaccharides longer than beta-1,4-mannobiose. It is thought to catalyze the phosphorolysis of beta-1,4-mannooligosaccharides longer than beta-1,4-mannobiose to produce alpha-D-mannose 1-phosphate and beta-1,4-mannobiose Ruminococcus albus [(1->4)-beta-D-mannosyl]n + phosphate
-
 r

Subunits

Subunits Comment Organism
homohexamer 6 * 38000, SDS-PAGE Ruminococcus albus
homohexamer 6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence Ruminococcus albus

Synonyms

Synonyms Comment Organism
RaMp2
-
 Ruminococcus albus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Ruminococcus albus
45
-
-
 Ruminococcus albus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable up to Ruminococcus albus
40
-
 stable below Ruminococcus albus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.06
-
 [(1->4)-beta-D-mannosyl]2 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
7.06
-
 beta-D-mannopyranosyl-(1->4)-D-mannopyranose pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
16
-
 [(1->4)-beta-D-mannosyl]6 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
16
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
20.5
-
 [(1->4)-beta-D-mannosyl]5 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
20.5
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
20.7
-
 4-O-beta-D-mannosyl-D-glucose pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
27.5
-
 [(1->4)-beta-D-mannosyl]3 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
27.5
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
31.2
-
 [(1->4)-beta-D-mannosyl]4 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
31.2
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
31.9
-
 [(1->4)-beta-D-mannosyl]5 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
31.9
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
33.1
-
 [(1->4)-beta-D-mannosyl]4 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
33.1
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
34.6
-
 [(1->4)-beta-D-mannosyl]3 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
34.6
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
45.1
-
 [(1->4)-beta-D-mannosyl]2 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
45.1
-
 beta-D-mannopyranosyl-(1->4)-D-mannopyranose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Ruminococcus albus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 9.5 stable Ruminococcus albus
3.5 9.5 4°C, 24 h stable Ruminococcus albus

General Information

General Information Comment Organism
physiological function the enzyme is involved in the metabolic pathway of mannan Ruminococcus albus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.159
-
 [(1->4)-beta-D-mannosyl]2 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
0.729
-
 4-O-beta-D-mannosyl-D-glucose pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
1.47
-
 [(1->4)-beta-D-mannosyl]2 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
2.6
-
 [(1->4)-beta-D-mannosyl]3 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
3.46
-
 [(1->4)-beta-D-mannosyl]3 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
3.57
-
 [(1->4)-beta-D-mannosyl]6 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
3.87
-
 [(1->4)-beta-D-mannosyl]5 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
4.11
-
 [(1->4)-beta-D-mannosyl]4 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
7.01
-
 [(1->4)-beta-D-mannosyl]5 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
10.3
-
 [(1->4)-beta-D-mannosyl]4 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus