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Literature summary for 2.4.1.329 extracted from
- The quest for a thermostable sucrose phosphorylase reveals sucrose 6'-phosphate phosphorylase as a novel specificity (2014), Appl. Microbiol. Biotechnol., 98, 7027-7037.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermoanaerobacterium thermosaccharolyticum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H344Y | decreased ratio of activity on phosphorylated fructose over fructose | Thermoanaerobacterium thermosaccharolyticum |
| K301A | displays similar activities as the wild type enzyme | Thermoanaerobacterium thermosaccharolyticum |
| R134A | decreased ratio of activity on phosphorylated fructose over fructose | Thermoanaerobacterium thermosaccharolyticum |
| R135A | displays similar activities as the wild type enzyme | Thermoanaerobacterium thermosaccharolyticum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.9 | - |
phosphate | pH 6.5, 55°C | Thermoanaerobacterium thermosaccharolyticum |  |
| 12.7 | - |
sucrose 6F-phosphate | pH 6.5, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
| 15.1 | - |
beta-D-fructofuranose 6-phosphate | pH 6.0, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
| 15.6 | - |
alpha-D-glucopyranose 1-phosphate | pH 6.0, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
| 41.6 | - |
beta-D-fructofuranose | pH 6.0, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
| 76.5 | - |
sucrose | pH 6.5, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 56000 SDS-PAGE, x * 56700, calculated | Thermoanaerobacterium thermosaccharolyticum |
| 56700 | - |
x * 56000 SDS-PAGE, x * 56700, calculated | Thermoanaerobacterium thermosaccharolyticum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose 6F-phosphate + phosphate | Thermoanaerobacterium thermosaccharolyticum | - |
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacterium thermosaccharolyticum | - |
- |
- |
| Thermoanaerobacterium thermosaccharolyticum | D9TT09 | - |
- |
| Thermoanaerobacterium thermosaccharolyticum DSM 571 | D9TT09 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose | - |
Thermoanaerobacterium thermosaccharolyticum | sucrose 6F-phosphate + phosphate | - |
? | |
| alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate | - |
Thermoanaerobacterium thermosaccharolyticum | sucrose 6F-phosphate + phosphate | - |
? | |
| alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate | - |
Thermoanaerobacterium thermosaccharolyticum | sucrose 6F-phosphate + phosphate | - |
r | |
| alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate | - |
Thermoanaerobacterium thermosaccharolyticum DSM 571 | sucrose 6F-phosphate + phosphate | - |
r | |
| additional information | no activity with trehalose 6-phosphate | Thermoanaerobacterium thermosaccharolyticum | ? | - |
? | |
| additional information | enzyme also acts on sucrose, reaction of EC 2.4.1.7, with lower activity. No substrate for phosphorolysis: trehalose 6-phosphate. No substrates for synthesis: D-psicose, alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, D-glucose, D-mannose, D-galactose, D-maltose, maltodextrin DE-15 | Thermoanaerobacterium thermosaccharolyticum | ? | - |
? | |
| additional information | enzyme also acts on sucrose, reaction of EC 2.4.1.7, with lower activity. No substrate for phosphorolysis: trehalose 6-phosphate. No substrates for synthesis: D-psicose, alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, D-glucose, D-mannose, D-galactose, D-maltose, maltodextrin DE-15 | Thermoanaerobacterium thermosaccharolyticum DSM 571 | ? | - |
? | |
| sucrose + phosphate | 51% compared to the activity with sucrose 6F-phosphate | Thermoanaerobacterium thermosaccharolyticum | alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose | - |
r | |
| sucrose 6F-phosphate + phosphate | - |
Thermoanaerobacterium thermosaccharolyticum | alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate | - |
? | |
| sucrose 6F-phosphate + phosphate | - |
Thermoanaerobacterium thermosaccharolyticum | alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate | - |
r | |
| sucrose 6F-phosphate + phosphate | - |
Thermoanaerobacterium thermosaccharolyticum DSM 571 | alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000 SDS-PAGE, x * 56700, calculated | Thermoanaerobacterium thermosaccharolyticum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sucrose 6'-phosphate phosphorylase | - |
Thermoanaerobacterium thermosaccharolyticum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Thermoanaerobacterium thermosaccharolyticum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life 60 h | Thermoanaerobacterium thermosaccharolyticum |
| 60 | - |
half-life: 60 h | Thermoanaerobacterium thermosaccharolyticum |
| 79 | - |
melting temperature | Thermoanaerobacterium thermosaccharolyticum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14.4 | - |
alpha-D-glucopyranose 1-phosphate | pH 6.0, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
| 16.9 | - |
beta-D-fructofuranose | pH 6.0, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
| 24.2 | - |
beta-D-fructofuranose 6-phosphate | pH 6.0, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
| 59.3 | - |
phosphate | pH 6.5, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
| 66.2 | - |
sucrose | pH 6.5, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
| 82.6 | - |
sucrose 6F-phosphate | pH 6.5, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
synthesis reaction | Thermoanaerobacterium thermosaccharolyticum |
| 6 | - |
synthetic reaction | Thermoanaerobacterium thermosaccharolyticum |
| 6.5 | - |
phosphorolysis | Thermoanaerobacterium thermosaccharolyticum |
| 6.5 | - |
phosphorolytic reaction | Thermoanaerobacterium thermosaccharolyticum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
beta-D-fructofuranose | pH 6.0, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
| 0.4 | - |
beta-D-fructofuranose 6-phosphate | pH 6.0, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
| 0.9 | - |
sucrose | pH 6.5, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
| 0.9 | - |
alpha-D-glucopyranose 1-phosphate | pH 6.0, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
| 1.6 | - |
beta-D-fructofuranose 6-phosphate | pH 6.0, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
| 6.5 | - |
sucrose 6F-phosphate | pH 6.5, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
| 8.6 | - |
phosphate | pH 6.5, 55°C | Thermoanaerobacterium thermosaccharolyticum | |
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