Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dodecylphosphoglycerol | anionic amphiphiles are essential for the restoration of a proper conformation. Amphiphilic environment with a critical fraction of negatively charged headgroups induces a catalytic, active site conformation of the enzyme | Acholeplasma laidlawii | |
dodecylphosphoglycerol | can stimulate the MGlcDAG synthase activity efficiently with a concomitant protection toward proteolytic digestion | Acholeplasma laidlawii | |
phosphatidylglycerol | anionic amphiphiles are essential for the restoration of a proper conformation. Amphiphilic environment with a critical fraction of negatively charged headgroups induces a catalytic, active site conformation of the enzyme | Acholeplasma laidlawii |
General Stability | Organism |
---|---|
total digestion by proteinase K, trypsin or chymotrypsin when no lipid is present, the activity is highly restored in micelles containing 1,2-dioleoyl-phosphatidylglycerol and 1,2-dioleoyl-phosphatidylserine | Acholeplasma laidlawii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Acholeplasma laidlawii | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPglucose + 1,2-diacylglycerol | Acholeplasma laidlawii | the enzyme synthesizes the major nonbilayer-prone lipid monoglucosyldiacylglycerol in the membrane, which is important for spontaneous curvature, and is an important site for the lipid surface charge density | UDP + 3-D-glucosyl-1,2-diacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acholeplasma laidlawii | - |
- |
- |
Acholeplasma laidlawii | Q93P60 | - |
- |
Purification (Comment) | Organism |
---|---|
proteolytic resistance shows a good correlation with the enzyme activity in various lipid-CHAPS mixed micelles. Anionic lipids 1,2-dioleoyl-phosphatidylglycerol and 1,2-dioleoyl-posphatidylserine are able to protect the exposed MGlc-DAG synthase from digestion, whereas 1,2-dioleoyl-phosphatidylcholine and diglucosyldiacylglycerol can not. The detergent dodecylphosphoglycerol can also stimulate the MGlcDAG synthase activity efficiently with a concomitant protection toward proteolytic digestion | Acholeplasma laidlawii |
Renatured (Comment) | Organism |
---|---|
critical fractions of anionic phospholipids 1,2-dioleoyl-phosphatidylglycerol and 1,2-dioleoyl-posphatidylserine are essential for the restoration of enzyme activity, while the zwitterionic 1,2-dioleoyl-phosphatidylcholine and the uncharged diglucosyldiacylglycerol are not | Acholeplasma laidlawii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPglucose + 1,2-diacylglycerol | - |
Acholeplasma laidlawii | UDP + 3-D-glucosyl-1,2-diacylglycerol | - |
? | |
UDPglucose + 1,2-diacylglycerol | the enzyme synthesizes the major nonbilayer-prone lipid monoglucosyldiacylglycerol in the membrane, which is important for spontaneous curvature, and is an important site for the lipid surface charge density | Acholeplasma laidlawii | UDP + 3-D-glucosyl-1,2-diacylglycerol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MGlcDAG synthase | - |
Acholeplasma laidlawii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
half-life in 1,2-dioleoylphosphatidylglycerol-CHAPS micelles is more than 2 h, half-life is less than 0.5 h in 1,2-dioleoylphosphatidylcholine-CHAPS micelles | Acholeplasma laidlawii |