Literature summary for 2.4.1.337 extracted from

Li, L.; Karlsson, O.P.; Wieslander, A.
Activating amphiphiles cause a conformational change of the 1,2-diacylglycerol 3-glucosyltransferase from Acholeplasma laidlawii membranes according to proteolytic digestion (1997), J. Biol. Chem., 272, 29602-29606.

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Activating Compound

Activating Compound	Comment	Organism
dodecylphosphoglycerol	anionic amphiphiles are essential for the restoration of a proper conformation. Amphiphilic environment with a critical fraction of negatively charged headgroups induces a catalytic, active site conformation of the enzyme	Acholeplasma laidlawii
dodecylphosphoglycerol	can stimulate the MGlcDAG synthase activity efficiently with a concomitant protection toward proteolytic digestion	Acholeplasma laidlawii
phosphatidylglycerol	anionic amphiphiles are essential for the restoration of a proper conformation. Amphiphilic environment with a critical fraction of negatively charged headgroups induces a catalytic, active site conformation of the enzyme	Acholeplasma laidlawii

General Stability

General Stability	Organism
total digestion by proteinase K, trypsin or chymotrypsin when no lipid is present, the activity is highly restored in micelles containing 1,2-dioleoyl-phosphatidylglycerol and 1,2-dioleoyl-phosphatidylserine	Acholeplasma laidlawii

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Acholeplasma laidlawii	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDPglucose + 1,2-diacylglycerol	Acholeplasma laidlawii	the enzyme synthesizes the major nonbilayer-prone lipid monoglucosyldiacylglycerol in the membrane, which is important for spontaneous curvature, and is an important site for the lipid surface charge density	UDP + 3-D-glucosyl-1,2-diacylglycerol	-	?

Organism

Organism	UniProt	Comment	Textmining
Acholeplasma laidlawii	-	-	-
Acholeplasma laidlawii	Q93P60	-	-

Purification (Commentary)

Purification (Comment)	Organism
proteolytic resistance shows a good correlation with the enzyme activity in various lipid-CHAPS mixed micelles. Anionic lipids 1,2-dioleoyl-phosphatidylglycerol and 1,2-dioleoyl-posphatidylserine are able to protect the exposed MGlc-DAG synthase from digestion, whereas 1,2-dioleoyl-phosphatidylcholine and diglucosyldiacylglycerol can not. The detergent dodecylphosphoglycerol can also stimulate the MGlcDAG synthase activity efficiently with a concomitant protection toward proteolytic digestion	Acholeplasma laidlawii

Purification (Comment)

Organism

proteolytic resistance shows a good correlation with the enzyme activity in various lipid-CHAPS mixed micelles. Anionic lipids 1,2-dioleoyl-phosphatidylglycerol and 1,2-dioleoyl-posphatidylserine are able to protect the exposed MGlc-DAG synthase from digestion, whereas 1,2-dioleoyl-phosphatidylcholine and diglucosyldiacylglycerol can not. The detergent dodecylphosphoglycerol can also stimulate the MGlcDAG synthase activity efficiently with a concomitant protection toward proteolytic digestion

Acholeplasma laidlawii

Renatured (Commentary)

Renatured (Comment)	Organism
critical fractions of anionic phospholipids 1,2-dioleoyl-phosphatidylglycerol and 1,2-dioleoyl-posphatidylserine are essential for the restoration of enzyme activity, while the zwitterionic 1,2-dioleoyl-phosphatidylcholine and the uncharged diglucosyldiacylglycerol are not	Acholeplasma laidlawii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDPglucose + 1,2-diacylglycerol	-	Acholeplasma laidlawii	UDP + 3-D-glucosyl-1,2-diacylglycerol	-	?
UDPglucose + 1,2-diacylglycerol	the enzyme synthesizes the major nonbilayer-prone lipid monoglucosyldiacylglycerol in the membrane, which is important for spontaneous curvature, and is an important site for the lipid surface charge density	Acholeplasma laidlawii	UDP + 3-D-glucosyl-1,2-diacylglycerol	-	?

Synonyms

Synonyms	Comment	Organism
MGlcDAG synthase	-	Acholeplasma laidlawii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
28	-	half-life in 1,2-dioleoylphosphatidylglycerol-CHAPS micelles is more than 2 h, half-life is less than 0.5 h in 1,2-dioleoylphosphatidylcholine-CHAPS micelles	Acholeplasma laidlawii