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Literature summary for 2.4.1.337 extracted from

  • Karlsson, O.P.; Dahlqvist, A.; Vikstroem, S.; Wieslander, A.
    Lipid dependence and basic kinetics of the purified 1,2-diacylglycerol 3-glucosyltransferase from membranes of Acholeplasma laidlawii (1997), J. Biol. Chem., 272, 929-936.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
CHAPS In CHAPS detergent, mixed micelles, enzyme shows a cooperative dependence on anionic lipids for activity Acholeplasma laidlawii
dioleoyl-phosphatidylglycerol stimulates the enzyme by an activating, potentially cooperative mechanism. Physiological concentrations of dioleoyl-phosphatidylglycerol influence the turnover number of the enzyme but not the interaction with UDP-glucose Acholeplasma laidlawii
sn-1,2-dioleoylglycerol activates Acholeplasma laidlawii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
UDP-alpha-D-glucose pH 8.0, 28°C, presence of 31 mol% CHAPS Acholeplasma laidlawii
0.39
-
UDP-alpha-D-glucose pH 8.0, 28°C, presence of 20 mol% CHAPS Acholeplasma laidlawii
0.44
-
UDP-alpha-D-glucose pH 8.0, 28°C, presence of 25 mol% CHAPS Acholeplasma laidlawii

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Acholeplasma laidlawii 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ strong dependence on, maximal activity at 15-28 mM MgCl2 Acholeplasma laidlawii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
1 * 40000, SDS-PAGE Acholeplasma laidlawii
40000
-
x * 40000, SDS-PAGE Acholeplasma laidlawii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPglucose + 1,2-diacylglycerol Acholeplasma laidlawii the product monoglucosyldiacylglycerol is the first glucolipid along the glucolipid pathway, and a major nonbilayer-prone lipid in the single membrane UDP + 3-D-glucosyl-1,2-diacylglycerol
-
?

Organism

Organism UniProt Comment Textmining
Acholeplasma laidlawii
-
-
-
Acholeplasma laidlawii Q93P60
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acholeplasma laidlawii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
-
Acholeplasma laidlawii

Storage Stability

Storage Stability Organism
-20°C or -50°C, stable for at least 48 days Acholeplasma laidlawii
-20°C, stable for at least 48 days Acholeplasma laidlawii
20°C, 100 mM KH2PO4/K2HPO4, pH 8, 20% (v/v) glycerol, and 20 mM CHAPS, half-life 1.8 h Acholeplasma laidlawii
4°C, 100 mM KH2PO4/K2HPO4, pH 8, 20% (v/v) glycerol, and 20 mM CHAPS, half-life 50h Acholeplasma laidlawii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + 1,2-dioleoyl-sn-glycerol
-
Acholeplasma laidlawii UDP + 1,2-dioleoyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
-
?
UDP-alpha-D-glucose + 1,2-dipalmitoyl-sn-glycerol
-
Acholeplasma laidlawii UDP + 1,2-dipalmitoyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
-
?
UDP-glucose + 1,2-dioleoylglycerol
-
Acholeplasma laidlawii UDP + 3-D-glucosyl-1,2-dioleoylglycerol
-
?
UDPglucose + 1,2-diacylglycerol
-
Acholeplasma laidlawii UDP + 3-D-glucosyl-1,2-diacylglycerol
-
?
UDPglucose + 1,2-diacylglycerol the product monoglucosyldiacylglycerol is the first glucolipid along the glucolipid pathway, and a major nonbilayer-prone lipid in the single membrane Acholeplasma laidlawii UDP + 3-D-glucosyl-1,2-diacylglycerol
-
?
UDPglucose + 1,2-dipalmitoylglycerol twice as high enzyme activity as sn-1,2-dioleoylglycerol at all concentrations up to 2.5 mol% concentration Acholeplasma laidlawii UDP + 3-D-glucosyl-1,2-dipalmitoylglycerol
-
?

Subunits

Subunits Comment Organism
? 1 * 40000, SDS-PAGE Acholeplasma laidlawii
? x * 40000, SDS-PAGE Acholeplasma laidlawii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
half-life is around 50 h Acholeplasma laidlawii
20
-
half-life is around 1.8 h Acholeplasma laidlawii