Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CHAPS | In CHAPS detergent, mixed micelles, enzyme shows a cooperative dependence on anionic lipids for activity | Acholeplasma laidlawii | |
dioleoyl-phosphatidylglycerol | stimulates the enzyme by an activating, potentially cooperative mechanism. Physiological concentrations of dioleoyl-phosphatidylglycerol influence the turnover number of the enzyme but not the interaction with UDP-glucose | Acholeplasma laidlawii | |
sn-1,2-dioleoylglycerol | activates | Acholeplasma laidlawii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
UDP-alpha-D-glucose | pH 8.0, 28°C, presence of 31 mol% CHAPS | Acholeplasma laidlawii | |
0.39 | - |
UDP-alpha-D-glucose | pH 8.0, 28°C, presence of 20 mol% CHAPS | Acholeplasma laidlawii | |
0.44 | - |
UDP-alpha-D-glucose | pH 8.0, 28°C, presence of 25 mol% CHAPS | Acholeplasma laidlawii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Acholeplasma laidlawii | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | strong dependence on, maximal activity at 15-28 mM MgCl2 | Acholeplasma laidlawii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
1 * 40000, SDS-PAGE | Acholeplasma laidlawii |
40000 | - |
x * 40000, SDS-PAGE | Acholeplasma laidlawii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPglucose + 1,2-diacylglycerol | Acholeplasma laidlawii | the product monoglucosyldiacylglycerol is the first glucolipid along the glucolipid pathway, and a major nonbilayer-prone lipid in the single membrane | UDP + 3-D-glucosyl-1,2-diacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acholeplasma laidlawii | - |
- |
- |
Acholeplasma laidlawii | Q93P60 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Acholeplasma laidlawii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
- |
Acholeplasma laidlawii |
Storage Stability | Organism |
---|---|
-20°C or -50°C, stable for at least 48 days | Acholeplasma laidlawii |
-20°C, stable for at least 48 days | Acholeplasma laidlawii |
20°C, 100 mM KH2PO4/K2HPO4, pH 8, 20% (v/v) glycerol, and 20 mM CHAPS, half-life 1.8 h | Acholeplasma laidlawii |
4°C, 100 mM KH2PO4/K2HPO4, pH 8, 20% (v/v) glycerol, and 20 mM CHAPS, half-life 50h | Acholeplasma laidlawii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + 1,2-dioleoyl-sn-glycerol | - |
Acholeplasma laidlawii | UDP + 1,2-dioleoyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol | - |
? | |
UDP-alpha-D-glucose + 1,2-dipalmitoyl-sn-glycerol | - |
Acholeplasma laidlawii | UDP + 1,2-dipalmitoyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol | - |
? | |
UDP-glucose + 1,2-dioleoylglycerol | - |
Acholeplasma laidlawii | UDP + 3-D-glucosyl-1,2-dioleoylglycerol | - |
? | |
UDPglucose + 1,2-diacylglycerol | - |
Acholeplasma laidlawii | UDP + 3-D-glucosyl-1,2-diacylglycerol | - |
? | |
UDPglucose + 1,2-diacylglycerol | the product monoglucosyldiacylglycerol is the first glucolipid along the glucolipid pathway, and a major nonbilayer-prone lipid in the single membrane | Acholeplasma laidlawii | UDP + 3-D-glucosyl-1,2-diacylglycerol | - |
? | |
UDPglucose + 1,2-dipalmitoylglycerol | twice as high enzyme activity as sn-1,2-dioleoylglycerol at all concentrations up to 2.5 mol% concentration | Acholeplasma laidlawii | UDP + 3-D-glucosyl-1,2-dipalmitoylglycerol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 1 * 40000, SDS-PAGE | Acholeplasma laidlawii |
? | x * 40000, SDS-PAGE | Acholeplasma laidlawii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
half-life is around 50 h | Acholeplasma laidlawii |
20 | - |
half-life is around 1.8 h | Acholeplasma laidlawii |