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Literature summary for 2.4.1.338 extracted from

  • Bai, L.; Li, L.; Xu, H.; Minagawa, K.; Yu, Y.; Zhang, Y.; Zhou, X.; Floss, H.G.; Mahmud, T.; Deng, Z.
    Functional analysis of the validamycin biosynthetic gene cluster and engineered production of validoxylamine A (2006), Chem. Biol., 13, 387-397.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Streptomyces lividans Streptomyces hygroscopicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
-
Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + validoxylamine A Streptomyces hygroscopicus the role of valG in the glycosylation of validoxylamine to validamycin A is demonstrated in vitro by enzymatic assay UDP + validamycin A
-
?
UDP-alpha-D-glucose + validoxylamine A Streptomyces hygroscopicus 5008 the role of valG in the glycosylation of validoxylamine to validamycin A is demonstrated in vitro by enzymatic assay UDP + validamycin A
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus
-
-
-
Streptomyces hygroscopicus 5008
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His6-Tagged ValG Streptomyces hygroscopicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + validoxylamine A UDP-glucose is the most efficient glycosyl donor. Whereas GDP-glucose is less efficient, ADP-glucose iss much less efficient and TDP-glucose is the least efficient donor Streptomyces hygroscopicus ADP + validamycin A
-
?
ADP-alpha-D-glucose + validoxylamine A UDP-glucose is the most efficient glycosyl donor. Whereas GDP-glucose is less efficient, ADP-glucose iss much less efficient and TDP-glucose is the least efficient donor Streptomyces hygroscopicus 5008 ADP + validamycin A
-
?
GDP-alpha-D-glucose + validoxylamine A UDP-glucose is the most efficient glycosyl donor. Whereas GDP-glucose is less efficient, ADP-glucose iss much less efficient and TDP-glucose is the least efficient donor Streptomyces hygroscopicus GDP + validamycin A
-
?
GDP-alpha-D-glucose + validoxylamine A UDP-glucose is the most efficient glycosyl donor. Whereas GDP-glucose is less efficient, ADP-glucose iss much less efficient and TDP-glucose is the least efficient donor Streptomyces hygroscopicus 5008 GDP + validamycin A
-
?
TDP-alpha-D-glucose + validoxylamine A UDP-glucose is the most efficient glycosyl donor. Whereas GDP-glucose is less efficient, ADP-glucose iss much less efficient and TDP-glucose is the least efficient donor Streptomyces hygroscopicus TDP + validamycin A
-
?
TDP-alpha-D-glucose + validoxylamine A UDP-glucose is the most efficient glycosyl donor. Whereas GDP-glucose is less efficient, ADP-glucose iss much less efficient and TDP-glucose is the least efficient donor Streptomyces hygroscopicus 5008 TDP + validamycin A
-
?
UDP-alpha-D-glucose + validoxylamine A the role of valG in the glycosylation of validoxylamine to validamycin A is demonstrated in vitro by enzymatic assay Streptomyces hygroscopicus UDP + validamycin A
-
?
UDP-alpha-D-glucose + validoxylamine A UDP-glucose is the most efficient glycosyl donor. Whereas GDP-glucose is less efficient, ADP-glucose iss much less efficient and TDP-glucose is the least efficient donor Streptomyces hygroscopicus UDP + validamycin A
-
?
UDP-alpha-D-glucose + validoxylamine A the role of valG in the glycosylation of validoxylamine to validamycin A is demonstrated in vitro by enzymatic assay Streptomyces hygroscopicus 5008 UDP + validamycin A
-
?
UDP-alpha-D-glucose + validoxylamine A UDP-glucose is the most efficient glycosyl donor. Whereas GDP-glucose is less efficient, ADP-glucose iss much less efficient and TDP-glucose is the least efficient donor Streptomyces hygroscopicus 5008 UDP + validamycin A
-
?

Subunits

Subunits Comment Organism
? x * 49000, polyhistidine-tagged protein, SDS-PAGE Streptomyces hygroscopicus

Synonyms

Synonyms Comment Organism
valG
-
Streptomyces hygroscopicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Streptomyces hygroscopicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Streptomyces hygroscopicus

General Information

General Information Comment Organism
malfunction in vivo inactivation of the putative glycosyltransferase gene (valG) abolished the final attachment of glucose for validamycin A production and results in accumulation of the VAL-A precursor, validoxylamine Streptomyces hygroscopicus
metabolism the enzyme is involved in biosynthesis of validamycin A, a weakly basic C7N-aminocyclitol-containing antibiotic Streptomyces hygroscopicus