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Literature summary for 2.4.1.338 extracted from

  • Singh, D.; Kwon, H.J.; Rajkarnikar, A.; Suh, J.W.
    Glucoamylase gene, vldI, is linked to validamycin biosynthesis in Streptomyces hygroscopicus var. limoneus, and vldADEFG confers validamycin production in Streptomyces lividans, revealing the role of VldE in glucose attachment (2007), Gene, 395, 151-159.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces hygroscopicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 x * 56000, calculated from amino acid sequence Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + validoxylamine A Streptomyces hygroscopicus
-
 UDP + validamycin A
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus Q15JG1 variant limoneus
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography, and Superdex 200 gel filtration Streptomyces hygroscopicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + validoxylamine A
-
 Streptomyces hygroscopicus UDP + validamycin A
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, calculated from amino acid sequence Streptomyces hygroscopicus

Synonyms

Synonyms Comment Organism
vldE
-
 Streptomyces hygroscopicus