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Literature summary for 2.4.1.350 extracted from
- Functional characterization of cucurbitadienol synthase and triterpene glycosyltransferase involved in biosynthesis of mogrosides from Siraitia grosvenorii (2015), Plant Cell Physiol., 56, 1172-1182 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta-gami (DE3) cells | Siraitia grosvenorii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0414 | - |
mogrol | at pH 7.2 and 30°C | Siraitia grosvenorii |  |
| 0.0547 | - |
naringenin | at pH 7.2 and 30°C | Siraitia grosvenorii | |
| 0.0582 | - |
quercetin | at pH 7.2 and 30°C | Siraitia grosvenorii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + mogrol | Siraitia grosvenorii | - |
mogroside IE + UDP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Siraitia grosvenorii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Siraitia grosvenorii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Siraitia grosvenorii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + mogrol | - |
Siraitia grosvenorii | mogroside IE + UDP | - |
? | |
| UDP-alpha-D-glucose + naringenin | - |
Siraitia grosvenorii | ? | - |
? | |
| UDP-alpha-D-glucose + quercetin | - |
Siraitia grosvenorii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52000, SDS-PAGE | Siraitia grosvenorii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UGT74AC1 | - |
Siraitia grosvenorii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000411 | - |
mogrol | at pH 7.2 and 30°C | Siraitia grosvenorii | |
| 0.41 | - |
quercetin | at pH 7.2 and 30°C | Siraitia grosvenorii | |
| 0.45 | - |
naringenin | at pH 7.2 and 30°C | Siraitia grosvenorii | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00994 | - |
mogrol | at pH 7.2 and 30°C | Siraitia grosvenorii | |
| 6.88 | - |
quercetin | at pH 7.2 and 30°C | Siraitia grosvenorii | |
| 8.23 | - |
naringenin | at pH 7.2 and 30°C | Siraitia grosvenorii | |
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Release 2023.1 (January 2023)
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