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Literature summary for 2.4.1.368 extracted from
- Biosynthesis of glycyrrhetinic acid-3-O-monoglucose using glycosyltransferase UGT73C11 from Barbarea vulgaris (2017), Ind. Eng. Chem. Res., 56, 14949-14958 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Barbarea vulgaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | about 68% residual activity at 5 mM | Barbarea vulgaris |  |
| Cu2+ | complete inhibition at 5 mM | Barbarea vulgaris | |
| Fe2+ | about 85% residual activity at 5 mM | Barbarea vulgaris | |
| additional information | not inhibited by EDTA | Barbarea vulgaris | |
| Zn2+ | about 22% residual activity at 5 mM | Barbarea vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.104 | - |
glycyrrhetinic acid | at pH 7.4 and 37°C | Barbarea vulgaris | |
| 0.486 | - |
UDP-alpha-D-glucose | at pH 7.4 and 37°C | Barbarea vulgaris | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | about 110% activity at 5 mM | Barbarea vulgaris | |
| additional information | not influenced by Ba2+, Ni2+, Mn2+, and Ca2+ | Barbarea vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Barbarea vulgaris | K4GGT4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Barbarea vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with UDP-glucuronic acid | Barbarea vulgaris | ? | - |
? | |
| UDP-alpha-D-glucose + glycyrrhetinic acid | the highest conversion is found at the substrate molar ratio UDP-alpha-D-glucose/glycyrrhetinate of 5:1 with 98% conversion after 6 h | Barbarea vulgaris | UDP + glycyrrhetinic acid 3-O-beta-monoglucose | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000, SDS-PAGE | Barbarea vulgaris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UGT73C11 | - |
Barbarea vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Barbarea vulgaris |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | more than 50% activity between 25 and 45°C | Barbarea vulgaris |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.091 | - |
glycyrrhetinic acid | at pH 7.4 and 37°C | Barbarea vulgaris | |
| 0.102 | - |
UDP-alpha-D-glucose | at pH 7.4 and 37°C | Barbarea vulgaris | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Barbarea vulgaris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | more than 60% activity between pH 6.0 and 8.0 | Barbarea vulgaris |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.215 | - |
UDP-alpha-D-glucose | at pH 7.4 and 37°C | Barbarea vulgaris | |
| 0.873 | - |
glycyrrhetinic acid | at pH 7.4 and 37°C | Barbarea vulgaris | |
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Release 2023.1 (January 2023)
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