Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.49 extracted from

  • Liu, N.; Fosses, A.; Kampik, C.; Parsiegla, G.; Denis, Y.; Vita, N.; Fierobe, H.P.; Perret, S.
    In vitro and in vivo exploration of the cellobiose and cellodextrin phosphorylases panel in Ruminiclostridium cellulolyticum implication for cellulose catabolism (2019), Biotechnol. Biofuels, 12, 208 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.49

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Ruminiclostridium cellulolyticum

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of structure Ruminiclostridium cellulolyticum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.7
-
 cellotetraose pH 7, 37°C Ruminiclostridium cellulolyticum
5.4
-
 cellotriose pH 7, 37°C Ruminiclostridium cellulolyticum
5.6
-
 cellopentaose pH 7, 37°C Ruminiclostridium cellulolyticum
11.9
-
 cellotetraose pH 7, 37°C Ruminiclostridium cellulolyticum

Organism

Organism UniProt Comment Textmining
Ruminiclostridium cellulolyticum B8I1R5 isoform CbpB
-
Ruminiclostridium cellulolyticum B8I1W7 isoform CbpA
-
Ruminiclostridium cellulolyticum B8I5F2 isoform CbpB
-
Ruminiclostridium cellulolyticum DSM 5812 B8I1R5 isoform CbpB
-
Ruminiclostridium cellulolyticum DSM 5812 B8I1W7 isoform CbpA
-
Ruminiclostridium cellulolyticum DSM 5812 B8I5F2 isoform CbpB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellopentaose + phosphate
-
 Ruminiclostridium cellulolyticum cellotetraose + alpha-D-glucose 1-phosphate
-
 ?
cellopentaose + phosphate poor substrate Ruminiclostridium cellulolyticum cellotetraose + alpha-D-glucose 1-phosphate
-
 ?
cellopentaose + phosphate poor substrate Ruminiclostridium cellulolyticum DSM 5812 cellotetraose + alpha-D-glucose 1-phosphate
-
 ?
cellopentaose + phosphate
-
 Ruminiclostridium cellulolyticum DSM 5812 cellotetraose + alpha-D-glucose 1-phosphate
-
 ?
cellotetraose + phosphate
-
 Ruminiclostridium cellulolyticum cellotriose + alpha-D-glucose 1-phosphate
-
 ?
cellotetraose + phosphate poor substrate Ruminiclostridium cellulolyticum cellotriose + alpha-D-glucose 1-phosphate
-
 ?
cellotetraose + phosphate poor substrate Ruminiclostridium cellulolyticum DSM 5812 cellotriose + alpha-D-glucose 1-phosphate
-
 ?
cellotetraose + phosphate
-
 Ruminiclostridium cellulolyticum DSM 5812 cellotriose + alpha-D-glucose 1-phosphate
-
 ?
cellotriose + phosphate
-
 Ruminiclostridium cellulolyticum cellobiose + alpha-D-glucose 1-phosphate
-
 ?
cellotriose + phosphate poor substrate Ruminiclostridium cellulolyticum cellobiose + alpha-D-glucose 1-phosphate
-
 ?
cellotriose + phosphate
-
 Ruminiclostridium cellulolyticum DSM 5812 cellobiose + alpha-D-glucose 1-phosphate
-
 ?
cellotriose + phosphate poor substrate Ruminiclostridium cellulolyticum DSM 5812 cellobiose + alpha-D-glucose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 90500, SDS-PAGE and calculated from sequence Ruminiclostridium cellulolyticum
? x * 91000, SDS-PAGE and calculated from sequence Ruminiclostridium cellulolyticum

Synonyms

Synonyms Comment Organism
CbpA
-
 Ruminiclostridium cellulolyticum
Ccel_1439
-
 Ruminiclostridium cellulolyticum
Ccel_2354
-
 Ruminiclostridium cellulolyticum
Ccel_3412
-
 Ruminiclostridium cellulolyticum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 cellotetraose pH 7, 37°C Ruminiclostridium cellulolyticum
39
-
 cellotetraose pH 7, 37°C Ruminiclostridium cellulolyticum
47.8
-
 cellopentaose pH 7, 37°C Ruminiclostridium cellulolyticum
127.5
-
 cellotriose pH 7, 37°C Ruminiclostridium cellulolyticum

Expression

Organism Comment Expression
Ruminiclostridium cellulolyticum only the expression of the gene encoding the cellobiose phosphorylase and the cellodextrin phosphorlyase isoform CdpA is induced during cellulose growth additional information

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.21
-
 cellotetraose pH 7, 37°C Ruminiclostridium cellulolyticum
3.29
-
 cellotetraose pH 7, 37°C Ruminiclostridium cellulolyticum
8.59
-
 cellopentaose pH 7, 37°C Ruminiclostridium cellulolyticum
23.5
-
 cellotriose pH 7, 37°C Ruminiclostridium cellulolyticum